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[192332_s_at] alcohol/ribitol dehydrogenase

Description C04F6.5 /REP_DB=WormBase Gene ID /WP=CE03925 /TR=SW:Q11177 /GB=AAA83589.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=alcohol-ribitol dehydrogenase
Cluster 6grp_highest_10_10 > 02      6grp_high1_10_10 > 10      6grp_highest_nonorm > 51      6grp_total > 50      4grp_total > 51      6grp_cgc > 60      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 583
1 2 3 4 range
A 280.7
(-0.6)
P 		583.1
(3)
P 		508.8
(2.1)
P 		73.6
(-3)
A 		510
B 362.8
(0.6)
P 		592.4
(3)
P 		515.6
(2.2)
P 		9.7
(-3)
A 		583
C 389.4
(1.4)
P 		514.6
(3)
P 		465.1
(2.4)
P 		57.1
(-3)
A 		458
avr 344.3
(0.5) 		563.4
(3) 		496.5
(2.2) 		46.8
(-3) 		517

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 828
1 2 3 4 5 6 range
A 965.8
(3)
P 		401
(-1.5)
P 		637.3
(0.4)
P 		690.6
(0.8)
P 		363.1
(-1.8)
P 		211.8
(-3)
P 		754
B 733.9
(2.9)
P 		419
(-0.4)
P 		622.6
(1.7)
P 		747.6
(3)
P 		395.2
(-0.7)
P 		172.8
(-3)
P 		575
C 673.9
(2.2)
P 		400.9
(-0.5)
P 		759.8
(3)
P 		648
(1.9)
P 		326
(-1.2)
P 		137.9
(-3)
P 		622
avr 791.2
(3) 		407
(-0.7) 		673.2
(1.9) 		695.4
(2.1) 		361.4
(-1.2) 		174.2
(-3) 		617
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 160
1 2 3 4 range
A 102.2
(1.5)
P 		126.6
(2.7)
P 		133
(3)
P 		10.8
(-3)
A 		122
B 115.7
(1.7)
P 		146.3
(3)
P 		119.5
(1.9)
P 		2.1
(-3)
A 		144
C 128.9
(1.7)
P 		148
(2.4)
P 		162.1
(3)
P 		9.6
(-3)
A 		153
avr 115.6
(1.9) 		140.3
(3) 		138.2
(2.9) 		7.5
(-3) 		133

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 209
1 2 3 4 5 6 range
A 178
(3)
P 		142.6
(1.3)
P 		138.8
(1.1)
P 		175.1
(2.9)
P 		109.6
(-0.2)
P 		51.6
(-3)
P 		126
B 255.4
(3)
P 		139.5
(-0.6)
P 		151.2
(-0.3)
P 		198
(1.2)
P 		160.6
(0)
P 		64.4
(-3)
P 		191
C 249.8
(3)
P 		122.3
(-0.8)
P 		193.4
(1.3)
P 		130.3
(-0.5)
P 		105.5
(-1.3)
P 		46.1
(-3)
P 		204
avr 227.7
(3) 		134.8
(-0.2) 		161.1
(0.7) 		167.8
(0.9) 		125.2
(-0.5) 		54
(-3) 		174
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192332_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18425
Sequence Type Exemplar sequence
Representative Public ID C04F6.5 NCBI
Target Description C04F6.5 /REP_DB=WormBase Gene ID /WP=CE03925 /TR=SW:Q11177 /GB=AAA83589.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=alcohol-ribitol dehydrogenase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076190(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076190 NCBI Caenorhabditis elegans DeHydrogenases, Short chain family member (dhs-27) (dhs-27) mRNA, complete cds. 11/11 None
SNAP00000029422 ENSEMBL cdna:SNAP chromosome:CEL140:X:3412964:3414693:-1 10/11 None
GENEFINDER00000029428 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:3412964:3414693:-1 11/11 None
C04F6.5 ENSEMBL cdna:known chromosome:CEL140:X:3412840:3418986:-1 gene:C04F6.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:3412964-3418987(-) 100.0 100.0
Public Domain and Genome References
Gene Title alcohol/ribitol dehydrogenase
Gene Symbol dhs-27
Ensembl C04F6.5
Entrez Gene 180632 Entrez gene
SwissProt Q11177 EMBL-EBI
WormBase CE37079 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA83589 Dehydrogenases, short chain protein 27 [Caenorhabditis elegans] ref|NP_508591.2| DeHydrogenases, Short chain family member (dhs-27) [Caenorhabditis elegans] sp|Q11177|DHS27_CAEEL Putative oxidoreductase dhs-27 0.0
blast CAE68425 Hypothetical protein CBG14205 [Caenorhabditis briggsae] 0.0
blast CAE68425 Hypothetical protein CBG14205 [Caenorhabditis briggsae] 1.0E-148
blast AAA83589 Dehydrogenases, short chain protein 27 [Caenorhabditis elegans] ref|NP_508591.2| DeHydrogenases, Short chain family member (dhs-27) [Caenorhabditis elegans] sp|Q11177|DHS27_CAEEL Putative oxidoreductase dhs-27 1.0E-127
blast CAE68425 Hypothetical protein CBG14205 [Caenorhabditis briggsae] 1.0E-118
blast AAA83589 Dehydrogenases, short chain protein 27 [Caenorhabditis elegans] ref|NP_508591.2| DeHydrogenases, Short chain family member (dhs-27) [Caenorhabditis elegans] sp|Q11177|DHS27_CAEEL Putative oxidoreductase dhs-27 5.0E-86
blast AAK68388 Dehydrogenases, short chain protein 5 [Caenorhabditis elegans] ref|NP_495035.1| DeHydrogenases, Short chain family member (dhs-5) [Caenorhabditis elegans] sp|Q10130|DHS5_CAEEL Putative oxidoreductase dhs-5 1.0E-35
blast AAK68388 Dehydrogenases, short chain protein 5 [Caenorhabditis elegans] ref|NP_495035.1| DeHydrogenases, Short chain family member (dhs-5) [Caenorhabditis elegans] sp|Q10130|DHS5_CAEEL Putative oxidoreductase dhs-5 2.0E-35
Protein Domains
Database ID Description E-Value
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 3.3E-18
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 3.3E-18
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_508591.2 2 744-766,778-800
C04F6.5 2 744-766,778-800
SNAP00000029422 1 272-294
GENEFINDER00000029428 1 282-304
Sequence
Target Sequence 
>C. ELEGANS:192332_S_AT
ttggaacactgaccgagctgccagaaggattggcgtccaagattcttcgtgttaacctga
tgtctgctgttaagatgaccgagatgattctgccaaacatggtgaagaaaaagcgaggaa
tcattgtaaatatctcatcaatgacgggctggaggcgcttccgtacctcagttcttaccc
ggctagcaaagctgcattatccttcttctccgactccttgtcagatgaataccgaggaac
tggaattcgtgttcaggtactgtctcattccaatgctcgttgctaccaaggtcgcctcgt
atgaagctgaagaagcgaacaatatcttcgttgtcacccctgaaaactttgccaaacaag
ctgtcagaattatcgggactaattgggaaatcacaacgggctgtgtgcaacatgatgttc
aggttgcccttggaacattgttctccttttggtttttcaaagtacttttcgtccctgttg
ttatgctcggagttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGGAACACTGACCGAGCTGCCAGA 366 709 1796 Antisense
GATTCTTCGTGTTAACCTGATGTCT 211 431 1836 Antisense
ATCAATGACGGGCTGGAGGCGCTTC 152 25 1932 Antisense
AGTTCTTACCCGGCTAGCAAAGCTG 606 61 1965 Antisense
AGCAAAGCTGCATTATCCTTCTTCT 171 77 1980 Antisense
CTTCTCCGACTCCTTGTCAGATGAA 277 221 2000 Antisense
AGGTCGCCTCGTATGAAGCTGAAGA 35 59 2084 Antisense
TTGTCACCCCTGAAAACTTTGCCAA 390 685 2126 Antisense
AGGTTGCCCTTGGAACATTGTTCTC 640 49 2216 Antisense
TTCAAAGTACTTTTCGTCCCTGTTG 464 685 2251 Antisense
GTCCCTGTTGTTATGCTCGGAGTTC 454 475 2266 Antisense
Sequence Source Affymetrix Proprietary Database