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[192325_s_at] Sodium/phosphate cotransporter

Description ZK512.6 /REP_DB=WormBase Gene ID /WP=CE01109 /TR=SW:P34644 /GB=CAA80150.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Sodium-phosphate cotransporter
Cluster 6grp_high1_10_10 > 17      4grp_highest_8_8 > 59      4grp_high_8_8 > 25      6grp_total > 06      4grp_total > 38      6grp_cgc > 12      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 375
1 2 3 4 range
A 494.1
(3)
P 		128.1
(-2.9)
P 		119.3
(-3)
P 		223.9
(-1.3)
P 		375
B 430.7
(3)
P 		275.2
(-0.2)
P 		143
(-3)
P 		237.1
(-1)
P 		288
C 447.5
(3)
P 		288.9
(-0.2)
P 		153.9
(-3)
P 		176.8
(-2.5)
P 		294
avr 457.4
(3) 		230.7
(-1.3) 		138.7
(-3) 		212.6
(-1.6) 		319

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 353
1 2 3 4 5 6 range
A 296.4
(3)
P 		176.8
(0.4)
P 		108.1
(-1.2)
P 		100.5
(-1.3)
P 		107.8
(-1.2)
P 		25.5
(-3)
A 		271
B 378.4
(3)
P 		184.4
(-0.8)
P 		118.5
(-2.1)
P 		112.7
(-2.2)
P 		88.9
(-2.6)
P 		70.4
(-3)
P 		308
C 327.3
(3)
P 		175.6
(-0.4)
P 		108.5
(-1.8)
P 		60.2
(-2.9)
A 		96.5
(-2.1)
A 		55.8
(-3)
P 		272
avr 334
(3) 		178.9
(-0.3) 		111.7
(-1.7) 		91.1
(-2.1) 		97.7
(-2) 		50.6
(-3) 		283
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 152
1 2 3 4 range
A 179.9
(3)
P 		27.8
(-3)
P 		31.2
(-2.9)
P 		32.8
(-2.8)
P 		152
B 137.3
(3)
P 		68
(-1)
P 		33.1
(-3)
P 		50.5
(-2)
P 		104
C 148.1
(3)
P 		83.1
(-0.3)
P 		53.6
(-1.8)
P 		29.8
(-3)
P 		118
avr 155.1
(3) 		59.6
(-1.9) 		39.3
(-2.9) 		37.7
(-3) 		117

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 126
1 2 3 4 5 6 range
A 54.6
(2.1)
P 		62.9
(3)
P 		23.5
(-1.2)
P 		25.5
(-1)
P 		32.5
(-0.2)
P 		6.2
(-3)
A 		57
B 131.7
(3)
P 		61.4
(-1)
P 		28.8
(-2.9)
P 		29.8
(-2.8)
P 		36.1
(-2.4)
P 		26.3
(-3)
P 		105
C 121.3
(3)
P 		53.6
(-0.7)
P 		27.6
(-2.1)
P 		12.1
(-3)
A 		31.2
(-2)
A 		18.7
(-2.6)
P 		109
avr 102.5
(3) 		59.3
(-0) 		26.6
(-2.3) 		22.5
(-2.6) 		33.3
(-1.9) 		17.1
(-3) 		85
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192325_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7607
Sequence Type Exemplar sequence
Representative Public ID ZK512.6 NCBI
Target Description ZK512.6 /REP_DB=WormBase Gene ID /WP=CE01109 /TR=SW:P34644 /GB=CAA80150.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Sodium-phosphate cotransporter
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066622(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066622 NCBI Caenorhabditis elegans EATing: abnormal pharyngeal pumping family member (eat-4) (eat-4) mRNA, complete cds. 11/11 A 
SNAP00000002532 ENSEMBL cdna:SNAP chromosome:CEL140:III:9136822:9141275:1 11/11 None
GENEFINDER00000002542 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:9136822:9141275:1 11/11 None
ZK512.6 ENSEMBL cdna:known chromosome:CEL140:III:9136822:9141728:1 gene:ZK512.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9136817-9141271(+) 100.0 100.0
Public Domain and Genome References
Gene Title Sodium/phosphate cotransporter
Gene Symbol eat-4
Ensembl ZK512.6
Entrez Gene 176291 Entrez gene
SwissProt P34644 EMBL-EBI
WormBase CE01109 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1597604_AT similar to solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 cfa
CANINE_2:CFAAFFX.6443.1.S1_AT similar to solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 cfa
CANINE_2:CFAAFFX.6443.1.S1_S_AT similar to solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 cfa
DROSOPHILA_2:1632620_AT Vesicular glutamate transporter dm
DROSGENOME1:145640_AT Vesicular glutamate transporter dm
HG-U133_PLUS_2:204230_S_AT solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 hs
HG-U133A:204230_S_AT solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 hs
HG-FOCUS:204230_S_AT solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 hs
HG-U133A_2:204230_S_AT solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 hs
U133_X3P:G9945321_3P_A_AT solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 hs
U133_X3P:HS.6535.0.S2_3P_AT solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 hs
HG-U133A_2:204229_AT solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 hs
HG-U133_PLUS_2:204229_AT solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 hs
HG-U133A:204229_AT solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 hs
HG-U95AV2:36567_AT solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 hs
HG-U95AV2:36568_AT solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 hs
HG-U95E:67416_AT Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 hs
HU35KSUBA:W26981_AT Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 hs
RAE230A:1368986_AT solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 rn
RAT230_2:1368986_AT solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 rn
RAE230A:1368987_AT solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 rn
RAT230_2:1368987_AT solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 rn
RG-U34A:RC_AI144756_AT solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 rn
Protein Similarities
Method ID Description E-Value
blast CAA80150 Hypothetical protein ZK512.6 [Caenorhabditis elegans] ref|NP_499023.3| EATing: abnormal pharyngeal pumping family member (eat-4) [Caenorhabditis elegans] sp|P34644|YOQ6_CAEEL Hypothetical protein ZK512.6 in chromosome III 0.0
blast AAC64972 EAT-4 [Caenorhabditis elegans] 0.0
blast CAE62654 Hypothetical protein CBG06792 [Caenorhabditis briggsae] 0.0
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499023.3 12 71-93,126-143,150-167,177-199,212-234,239-261,306-325,340-362,382-401,405-427,432-454,469-491
ZK512.6 12 71-93,126-143,150-167,177-199,212-234,239-261,306-325,340-362,382-401,405-427,432-454,469-491
SNAP00000002532 12 71-93,126-143,150-167,177-199,212-234,239-261,306-325,340-362,382-401,405-427,432-454,469-491
GENEFINDER00000002542 12 71-93,126-143,150-167,177-199,212-234,239-261,306-325,340-362,382-401,405-427,432-454,469-491
Sequence
Target Sequence 
>C. ELEGANS:192325_S_AT
cgatccaacaagatcctttccaccactgccgttcggaagattttcaattgtggtggtttt
ggaggagaagctgcattcatgctaattgttgcatatacaacaagtgatacaactgctata
atggcattgattgctgctgttggaatgtctggatttgctatttctggtttcaacgtaaat
catttggacattgctccccgttatgcagccatcctaatgggattctcgaatggaatcgga
acacttgccggattaacttgcccatttgtgactgaagcattcactgctcactcgaaacac
ggatggacgagtgttttcttattagccagtcttattcactttactggagtcactttttat
gctgtctatgcttccggagaacttcaagaatgggcagaaccaaaagaggaggaagaatgg
tcgaataaggaattggtgaataaaactggaatcaatggaactggatacggagctgctgag
actacattcacacagttaccagccggtgttgattcttcttatcaagctcaggcgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGATCCAACAAGATCCTTTCCACCA 471 249 1117 Antisense
CCCGTTATGCAGCCATCCTAATGGG 96 261 1313 Antisense
ACACTTGCCGGATTAACTTGCCCAT 643 107 1357 Antisense
TTCACTGCTCACTCGAAACACGGAT 586 685 1396 Antisense
GAGTGTTTTCTTATTAGCCAGTCTT 458 401 1425 Antisense
GAGTCACTTTTTATGCTGTCTATGC 641 399 1463 Antisense
CTGTCTATGCTTCCGGAGAACTTCA 680 237 1478 Antisense
GAACTGGATACGGAGCTGCTGAGAC 281 349 1574 Antisense
GACTACATTCACACAGTTACCAGCC 192 373 1596 Antisense
GTTACCAGCCGGTGTTGATTCTTCT 453 445 1611 Antisense
GATTCTTCTTATCAAGCTCAGGCGG 592 429 1627 Antisense
Sequence Source Affymetrix Proprietary Database