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[192313_s_at]

Description T04A8.7 /REP_DB=WormBase Gene ID /WP=CE01076 /TR=Q22137 /GB=CAA84727.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=1,4-Alpha-glucan branching enzyme
Cluster 6grp_highest_10_10 > 24      6grp_high1_10_10 > 30      4grp_highest_8_8 > 32      4grp_high_8_8 > 43      6grp_highest_nonorm > 23      6grp_total > 42      4grp_total > 52      6grp_cgc > 82      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1757
1 2 3 4 range
A 2014.2
(2.4)
P 		2180.4
(3)
P 		1513.6
(0.6)
P 		529.9
(-3)
P 		1651
B 1857.6
(3)
P 		1813.4
(2.8)
P 		1721.3
(2.4)
P 		517.8
(-3)
P 		1340
C 2153
(3)
P 		1771.5
(1.7)
P 		1477.2
(0.7)
P 		423.9
(-3)
P 		1729
avr 2008.3
(3) 		1921.8
(2.7) 		1570.7
(1.3) 		490.5
(-3) 		1518

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1970
1 2 3 4 5 6 range
A 2004.9
(-0.2)
P 		2235.2
(0.8)
P 		2710.7
(3)
P 		1963.2
(-0.4)
P 		1384.3
(-3)
P 		1638
(-1.9)
P 		1326
B 1769.5
(-1.3)
P 		2286.8
(0.5)
P 		2991
(3)
P 		1876.6
(-0.9)
P 		1278.3
(-3)
P 		1589.7
(-1.9)
P 		1713
C 1764.2
(-1.8)
P 		2228.9
(-0.3)
P 		3248.5
(3)
P 		2035.2
(-0.9)
P 		1382.4
(-3)
P 		1490.6
(-2.7)
P 		1866
avr 1846.2
(-1.2) 		2250.3
(0.3) 		2983.4
(3) 		1958.3
(-0.8) 		1348.3
(-3) 		1572.8
(-2.2) 		1635
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 662
1 2 3 4 range
A 733.4
(3)
P 		473.3
(0.6)
P 		395.7
(-0.1)
P 		77.6
(-3)
P 		656
B 592.4
(3)
P 		447.9
(1.2)
P 		398.9
(0.6)
P 		110.3
(-3)
P 		482
C 712.7
(3)
P 		509.3
(1.1)
P 		514.9
(1.1)
P 		71.5
(-3)
P 		641
avr 679.5
(3) 		476.8
(0.9) 		436.5
(0.5) 		86.5
(-3) 		593

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 457
1 2 3 4 5 6 range
A 369.6
(-3)
P 		794.9
(3)
P 		590.3
(0.1)
P 		497.9
(-1.2)
P 		417.7
(-2.3)
P 		398.8
(-2.6)
P 		425
B 615.7
(-0.3)
P 		761.2
(3)
P 		726.3
(2.2)
P 		497.1
(-3)
P 		519.3
(-2.5)
P 		592.8
(-0.8)
P 		264
C 653.9
(0.5)
P 		680.1
(0.9)
P 		826.8
(3)
P 		409.3
(-3)
P 		447.3
(-2.5)
P 		499
(-1.7)
P 		418
avr 546.4
(-1.2) 		745.4
(3) 		714.5
(2.3) 		468.1
(-2.9) 		461.4
(-3) 		496.9
(-2.3) 		284
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192313_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6591
Sequence Type Exemplar sequence
Representative Public ID T04A8.7 NCBI
Target Description T04A8.7 /REP_DB=WormBase Gene ID /WP=CE01076 /TR=Q22137 /GB=CAA84727.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=1,4-Alpha-glucan branching enzyme
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065560(11), NM_181902(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065560 NCBI Caenorhabditis elegans T04A8.7a (T04A8.7) mRNA, complete cds. 11/11 None
NM_181902 NCBI Caenorhabditis elegans T04A8.7b (T04A8.7) mRNA, complete cds. 11/11 None
SNAP00000041095 ENSEMBL cdna:SNAP chromosome:CEL140:III:4695333:4697721:1 11/11 None
GENEFINDER00000041119 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4695333:4697721:1 11/11 None
T04A8.7a ENSEMBL cdna:known chromosome:CEL140:III:4695323:4697970:1 gene:T04A8.7 11/11 None
T04A8.7b ENSEMBL cdna:known chromosome:CEL140:III:4695625:4697721:1 gene:T04A8.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_065561 8/11 Cross Hyb Matching Probes None
T04A8.9.1 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4695332-4697721(+) 100.0 100.0
Public Domain and Genome References
Ensembl T04A8.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA84727 Hypothetical protein T04A8.7a [Caenorhabditis elegans] ref|NP_497961.1| T04A8.7a [Caenorhabditis elegans] 0.0
blast CAE71154 Hypothetical protein CBG18010 [Caenorhabditis briggsae] 0.0
blast CAD82917 Hypothetical protein T04A8.7b [Caenorhabditis elegans] ref|NP_871631.1| T04A8.7b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004193 EMBL-EBI Glycoside hydrolase, family 13, N-terminal 1.6E-25
Pfam IPR004193 EMBL-EBI Glycoside hydrolase, family 13, N-terminal 5.3E-12
Sequence
Target Sequence 
>C. ELEGANS:192313_S_AT
gacttcatgtctacagactcacctcttactccaattatcgacagaggattgtcccttcac
aagttgatccgtcttattactattggattaggcggagaagcatggctcaatttcattggt
aacgaattcggacatcctgaatggctcgatttcccacgcgtcggaaacggagaatcattc
cattatgctcgtcgtcagttcaatcttgtcgatgccgaatatcttcgttacaaattcctg
aacaattgggatcgtgaaatgatgctgctagaggagcgtactggattccttcacaaggga
tacgcttacacatcatggaagcacgacggagacaaaactattgtctttgagagaggtggc
ttggtatttgtgatcaacttgcatccaactaaaagttttgctgactactcgattggagtt
aatactcctggaagataccgaatcgctcttaattctgatgagtcaaagtttggaggccac
aaccgtatcgacaactccatcaaattccacaccactgatgatggatacgctggtagacgt
catcgtcttcaagtttacatcacatgccgcac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACTTCATGTCTACAGACTCACCTC 622 371 1456 Antisense
TCACCTCTTACTCCAATTATCGACA 22 627 1474 Antisense
GATTGTCCCTTCACAAGTTGATCCG 221 435 1502 Antisense
GACATCCTGAATGGCTCGATTTCCC 11 371 1586 Antisense
GAATCATTCCATTATGCTCGTCGTC 161 329 1627 Antisense
GTCAGTTCAATCTTGTCGATGCCGA 110 467 1649 Antisense
GAGGAGCGTACTGGATTCCTTCACA 198 405 1726 Antisense
TTTGTGATCAACTTGCATCCAACTA 215 665 1822 Antisense
AGTTTGGAGGCCACAACCGTATCGA 139 1 1922 Antisense
GGATACGCTGGTAGACGTCATCGTC 270 509 1978 Antisense
TTCAAGTTTACATCACATGCCGCAC 39 685 2003 Antisense
Sequence Source Affymetrix Proprietary Database