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[192311_at] LET-60 RAS protein

Description ZK792.6 /REP_DB=WormBase Gene ID /WP=CE03827 /GEN=let-60 /TR=SW:P22981 /GB=CAA92630.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=LET-60 RAS protein
Cluster 6grp_highest_10_10 > 21      6grp_high1_10_10 > 02      4grp_highest_8_8 > 41      4grp_high_8_8 > 49      6grp_highest_nonorm > 31      6grp_total > 24      4grp_total > 54      6grp_highest_sub > De      6grp_cgc > 20      6grp_high1_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 586
1 2 3 4 range
A 978.3
(3)
P 		748.2
(-0.5)
P 		719.3
(-0.9)
P 		583.3
(-3)
P 		395
B 1077
(3)
P 		676.8
(-3)
P 		751.9
(-1.9)
P 		675.1
(-3)
P 		402
C 988.6
(3)
P 		955.5
(2.6)
P 		797.1
(0.7)
P 		490.7
(-3)
P 		498
avr 1014.6
(3) 		793.5
(-0.1) 		756.1
(-0.6) 		583
(-3) 		432

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 993
1 2 3 4 5 6 range
A 828.2
(0.1)
P 		1079.5
(3)
P 		743.4
(-0.8)
P 		826.5
(0.1)
P 		550.5
(-3)
P 		746.6
(-0.8)
P 		529
B 885
(2.6)
P 		829.2
(1.7)
P 		911.8
(3)
P 		734.1
(0.1)
P 		616.4
(-1.8)
P 		540.2
(-3)
P 		372
C 1507.1
(3)
P 		815.5
(-1.2)
P 		959.9
(-0.3)
P 		898.9
(-0.7)
P 		668.1
(-2.1)
P 		513.8
(-3)
P 		993
avr 1073.4
(3) 		908.1
(0.9) 		871.7
(0.4) 		819.8
(-0.2) 		611.7
(-2.9) 		600.2
(-3) 		473
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 274
1 2 3 4 range
A 356.2
(3)
P 		162.4
(-1.3)
P 		188
(-0.7)
P 		85.4
(-3)
P 		271
B 343.5
(3)
P 		167.2
(-2.3)
P 		174.2
(-2.1)
P 		143.9
(-3)
P 		200
C 327.2
(3)
P 		274.7
(1.7)
P 		277.8
(1.8)
P 		82.7
(-3)
P 		245
avr 342.3
(3) 		201.4
(-0.5) 		213.3
(-0.2) 		104
(-3) 		238

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 406
1 2 3 4 5 6 range
A 152.7
(-3)
P 		383.9
(3)
P 		161.9
(-2.8)
P 		209.6
(-1.5)
P 		166.1
(-2.7)
P 		181.8
(-2.2)
P 		231
B 307.9
(3)
P 		276
(1.3)
P 		221.4
(-1.6)
P 		194.4
(-3)
P 		250.4
(-0)
P 		201.4
(-2.6)
P 		114
C 558.6
(3)
P 		248.8
(-1.8)
P 		244.3
(-1.9)
P 		180.8
(-2.9)
P 		216.2
(-2.3)
P 		172
(-3)
P 		387
avr 339.7
(3) 		302.9
(1.6) 		209.2
(-2.1) 		194.9
(-2.6) 		210.9
(-2) 		185.1
(-3) 		155
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192311_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9828
Sequence Type Exemplar sequence
Representative Public ID ZK792.6 NCBI
Target Description ZK792.6 /REP_DB=WormBase Gene ID /WP=CE03827 /GEN=let-60 /TR=SW:P22981 /GB=CAA92630.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=LET-60 RAS protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069812(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069812 NCBI Caenorhabditis elegans LEThal family member (let-60) (let-60) mRNA, complete cds. 11/11 A 
SNAP00000023874 ENSEMBL cdna:SNAP chromosome:CEL140:IV:11689016:11691047:-1 11/11 None
GENEFINDER00000023878 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:11688821:11691047:-1 11/11 None
ZK792.6 ENSEMBL cdna:known chromosome:CEL140:IV:11688197:11691058:-1 gene:ZK792.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11689021-11691053(-) 100.0 100.0
Public Domain and Genome References
Gene Title LET-60 RAS protein
Gene Symbol let-60
Ensembl ZK792.6
Entrez Gene 178104 Entrez gene
SwissProt P22981 EMBL-EBI
WormBase CE03827 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1624899_AT Ras oncogene at 85D dm
DROSGENOME1:143317_AT Ras oncogene at 85D dm
CHICKEN:GGAAFFX.24341.1.S1_AT similar to turkey K-Ras gga
HG-U95AV2:32159_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog hs
HG-U95AV2:1940_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog hs
HC-G110:1940_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog hs
HG-U133A:204010_S_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog hs
HG-U133_PLUS_2:204010_S_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog hs
HG-U133A_2:204010_S_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog hs
HU35KSUBC:RC_AA425857_S_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog hs
HU35KSUBD:RC_AA505084_S_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog hs
HUGENEFL:M54968_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog hs
U133_X3P:G4826811_3P_A_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog hs
U133_X3P:HS2.422851.1.A1_3P_X_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog hs
U133_X3P:HS2.422851.1.A1_3P_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog hs
U133_X3P:HS.184050.1.A1_3P_A_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog hs
U133_X3P:HS.184050.0.S1_3P_A_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog hs
U133_X3P:1559203_3P_S_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog hs
HG-U133_PLUS_2:1559203_S_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog hs
HG-U133_PLUS_2:1559204_X_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog hs
HG-U133A_2:204009_S_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog hs
HG-FOCUS:204009_S_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog hs
HG-U133_PLUS_2:204009_S_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog hs
HG-U133A:204009_S_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog hs
HG-U133A_2:214352_S_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog hs
HG-U133_PLUS_2:214352_S_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog hs
HG-U133A:214352_S_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog hs
HG-U95D:70329_AT V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog hs
MU11KSUBB:X02452_S_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog mm
MG-U74AV2:97991_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog mm
MG-U74BV2:106672_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog mm
MG-U74BV2:106938_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog mm
MOE430A:1426229_S_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog mm
MOUSE430A_2:1426229_S_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog mm
MOUSE430_2:1426229_S_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog mm
MOE430A:1426228_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog mm
MOUSE430A_2:1426228_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog mm
MOUSE430_2:1426228_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog mm
MOE430A:1434000_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog mm
MOUSE430A_2:1434000_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog mm
MOUSE430_2:1434000_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog mm
MOE430A:1451979_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog mm
MOUSE430A_2:1451979_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog mm
MOUSE430_2:1451979_AT v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog mm
MU19KSUBC:TC36682_AT V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (Kras), mRNA mm
MU19KSUBC:TC36682_G_AT V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (Kras), mRNA mm
RN-U34:U09793_AT Kirsten rat sarcoma viral oncogene homologue 2 (active) rn
RT-U34:U09793_AT Kirsten rat sarcoma viral oncogene homologue 2 (active) rn
RG-U34A:U09793_AT Kirsten rat sarcoma viral oncogene homologue 2 (active) rn
RAE230A:1370035_AT Kirsten rat sarcoma viral oncogene homologue 2 (active) rn
RAT230_2:1370035_AT Kirsten rat sarcoma viral oncogene homologue 2 (active) rn
RAE230A:1372355_AT Kirsten rat sarcoma viral oncogene homologue 2 (active) rn
RAT230_2:1372355_AT Kirsten rat sarcoma viral oncogene homologue 2 (active) rn
RG-U34B:RC_AA944575_AT Kirsten rat sarcoma viral oncogene homologue 2 (active) rn
RG-U34B:RC_AI045340_AT Kirsten rat sarcoma viral oncogene homologue 2 (active) rn
RG-U34C:RC_AI101656_AT Kirsten rat sarcoma viral oncogene homologue 2 (active) rn
RG-U34C:RC_AI229416_AT Kirsten rat sarcoma viral oncogene homologue 2 (active) rn
YEAST_2:1773513_AT GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6886 intracellular protein transport inferred from electronic annotation QuickGO AmiGO
7264 small GTPase mediated signal transduction inferred from electronic annotation QuickGO AmiGO
15031 protein transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA92630 Hypothetical protein ZK792.6 [Caenorhabditis elegans] ref|NP_502213.3| LEThal family member (let-60) [Caenorhabditis elegans] sp|P22981|LET60_CAEEL Ras protein let-60 (Lethal protein 60) 5.0E-95
blast AAA28103 ras protein 5.0E-95
blast CAE62069 Hypothetical protein CBG06092 [Caenorhabditis briggsae] 2.0E-94
blast CAA92630 Hypothetical protein ZK792.6 [Caenorhabditis elegans] ref|NP_502213.3| LEThal family member (let-60) [Caenorhabditis elegans] sp|P22981|LET60_CAEEL Ras protein let-60 (Lethal protein 60) 3.0E-82
Protein Domains
Database ID Description E-Value
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 3.0E-96
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000023878 1 173-195
Sequence
Target Sequence 
>C. ELEGANS:192311_AT
aactcatccagaatcactttgtcgaagaatacgacccgaccatagaggacagctacagaa
agcaggttgtgatagacggtgagacatgcctcctcgacatattggataccgccggacaag
aagaatattcggcgatgcgtgatcagtacatgaggacaggcgaaggatttctgttggttt
tcgccgtcaacgaggctaaatctttcgagaatgtcgctaactaccgcgagcagattcgga
gggtaaaggattcagatgatgttcctatggtcttggtagggaataaatgtgatttgtcat
ctcgatcagtcgacttccgaacagtcagtgagacagcaaagggttacggtattccgaatg
tcgacacatctgccaaaacgcgtat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACTCATCCAGAATCACTTTGTCGA 292 141 77 Antisense
TACGACCCGACCATAGAGGACAGCT 392 645 106 Antisense
GATAGACGGTGAGACATGCCTCCTC 457 405 147 Antisense
GCCTCCTCGACATATTGGATACCGC 705 281 164 Antisense
ATTCGGCGATGCGTGATCAGTACAT 424 7 203 Antisense
GAAGGATTTCTGTTGGTTTTCGCCG 335 333 238 Antisense
GTTTTCGCCGTCAACGAGGCTAAAT 124 447 253 Antisense
GAATGTCGCTAACTACCGCGAGCAG 387 331 285 Antisense
GATGATGTTCCTATGGTCTTGGTAG 209 411 331 Antisense
CATCTCGATCAGTCGACTTCCGAAC 353 211 374 Antisense
TCGACACATCTGCCAAAACGCGTAT 105 601 437 Antisense
Sequence Source Affymetrix Proprietary Database