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[192308_s_at] Serine/Threonine protein kinase

Description T07F12.4 /REP_DB=WormBase Gene ID /WP=CE02868 /TR=Q22324 /GB=AAA68420.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=Serine-Threonine protein kinase
Cluster 6grp_highest_10_10 > 72      6grp_high1_10_10 > 38      4grp_highest_8_8 > 62      4grp_high_8_8 > 19      6grp_highest_nonorm > 40      6grp_total > 37      4grp_total > 29      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 863
1 2 3 4 range
A 1349.2
(-0.1)
P 		1297.7
(-0.5)
P 		989.2
(-3)
P 		1727.9
(3)
P 		739
B 1471
(3)
P 		1051.6
(-1.4)
P 		897
(-3)
P 		1435.2
(2.6)
P 		574
C 1493.8
(3)
P 		1204.3
(0.2)
P 		865.4
(-3)
P 		1169.3
(-0.1)
P 		628
avr 1438
(2.9) 		1184.5
(0) 		917.2
(-3) 		1444.1
(3) 		527

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 843
1 2 3 4 5 6 range
A 1161.6
(3)
P 		702.2
(-0.7)
P 		427
(-2.9)
P 		415.8
(-3)
P 		499.8
(-2.3)
P 		792.2
(0)
P 		746
B 1221.6
(3)
P 		808.4
(-0.1)
P 		525.6
(-2.2)
P 		461.3
(-2.7)
P 		425.6
(-3)
P 		850.7
(0.2)
P 		796
C 990.4
(3)
P 		789.3
(1)
P 		413.5
(-2.7)
P 		431.6
(-2.5)
P 		379
(-3)
P 		848
(1.6)
P 		611
avr 1124.5
(3) 		766.6
(-0.1) 		455.4
(-2.8) 		436.2
(-3) 		434.8
(-3) 		830.3
(0.4) 		690
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 297
1 2 3 4 range
A 491.3
(3)
P 		281.7
(-2.3)
P 		258.6
(-2.9)
P 		252.9
(-3)
P 		238
B 469.1
(3)
P 		259.7
(-1.8)
P 		207.9
(-3)
P 		305.8
(-0.8)
P 		261
C 494.5
(3)
P 		346.2
(0)
P 		301.6
(-0.9)
P 		197.2
(-3)
P 		297
avr 485
(3) 		295.9
(-1.9) 		256
(-2.9) 		252
(-3) 		233

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 338
1 2 3 4 5 6 range
A 214.1
(1.6)
P 		249.7
(3)
P 		93
(-3)
P 		105.5
(-2.5)
P 		150.8
(-0.8)
P 		192.9
(0.8)
P 		157
B 425.1
(3)
P 		269.1
(-0.1)
P 		127.6
(-2.9)
P 		122.2
(-3)
P 		172.9
(-2)
P 		317.2
(0.9)
P 		303
C 367.1
(3)
P 		240.8
(0.3)
P 		105.2
(-2.6)
P 		86.8
(-3)
P 		122.6
(-2.2)
P 		283.9
(1.2)
P 		280
avr 335.4
(3) 		253.2
(0.9) 		108.6
(-2.9) 		104.8
(-3) 		148.8
(-1.9) 		264.7
(1.2) 		231
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192308_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16538
Sequence Type Exemplar sequence
Representative Public ID T07F12.4 NCBI
Target Description T07F12.4 /REP_DB=WormBase Gene ID /WP=CE02868 /TR=Q22324 /GB=AAA68420.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=Serine-Threonine protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076432(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076432 NCBI Caenorhabditis elegans T07F12.4 (T07F12.4) mRNA, complete cds. 11/11 None
SNAP00000035174 ENSEMBL cdna:SNAP chromosome:CEL140:X:4894191:4898131:-1 11/11 None
GENEFINDER00000035176 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:4896728:4898131:-1 11/11 None
T07F12.4 ENSEMBL cdna:known chromosome:CEL140:X:4896639:4898121:-1 gene:T07F12.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4896728-4897887(-) 100.0 100.0
Public Domain and Genome References
Gene Title Serine/Threonine protein kinase
Ensembl T07F12.4
Entrez Gene 180763 Entrez gene
SwissProt Q22324 EMBL-EBI
WormBase CE28650 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA68420 Hypothetical protein T07F12.4 [Caenorhabditis elegans] ref|NP_508833.1| T07F12.4 [Caenorhabditis elegans] 1.0E-168
blast AAA68420 Hypothetical protein T07F12.4 [Caenorhabditis elegans] ref|NP_508833.1| T07F12.4 [Caenorhabditis elegans] 1.0E-167
blast CAE68663 Hypothetical protein CBG14564 [Caenorhabditis briggsae] 1.0E-154
blast CAE68663 Hypothetical protein CBG14564 [Caenorhabditis briggsae] 1.0E-153
blast AAO01099 CG10967-PA [Drosophila virilis] 4.0E-31
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 3.0E-14
hanks 2.1.13 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; ACMPK 3.0E-19
hanks 2.1.13 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; ACMPK 2.0E-19
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 2.2E-51
Pfam IPR000719 EMBL-EBI Protein kinase 2.2E-51
Sequence
Target Sequence 
>C. ELEGANS:192308_S_AT
ttcgacgactttttctgctgtttaataatggaactctgcgattgtgatcttgaccatcac
atgcgcaatatttcggtcaaaggaagattgaatccgtcgaatttcaggcttcttcttgac
aacattgcccgaggatacaaagcgctttatgagttgaaaattgtacatcgggacattaaa
ccccaaaacatactgatcacttatacagatgcatcgaaacaaattgcttgtgctcggatc
acagactttggaatttcgagaacccttgataacgagggagaagagctgtgcaacgttgcg
ggtacattttattacatggctccagaagttggagcgaaccttttaaaaacttgtcagtat
gactcaaaagttgatatgtggagcatcggatgccttctctaccaatgcgttacaggagag
gtcccattcgatgagtgcagtttgtgtaagctcttcctgtatgtggcaggggctaacttc
gacgcctacgacccgcctgagctgcccgacgagctgagtcaagaggtttcggggattatt
caatcactgctgcagctcgataccactcaaagatgcacacctactcagttttat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCGACGACTTTTTCTGCTGTTTAA 665 695 103 Antisense
GATCTTGACCATCACATGCGCAATA 176 421 148 Antisense
GAATCCGTCGAATTTCAGGCTTCTT 374 329 192 Antisense
GGCTTCTTCTTGACAACATTGCCCG 612 537 209 Antisense
TGGAGCATCGGATGCCTTCTCTACC 231 549 481 Antisense
GAGGTCCCATTCGATGAGTGCAGTT 51 403 520 Antisense
GTGTAAGCTCTTCCTGTATGTGGCA 684 485 546 Antisense
TGTGGCAGGGGCTAACTTCGACGCC 258 555 564 Antisense
GATTATTCAATCACTGCTGCAGCTC 415 429 636 Antisense
TGCAGCTCGATACCACTCAAAGATG 641 577 653 Antisense
AAGATGCACACCTACTCAGTTTTAT 442 155 672 Antisense
Sequence Source Affymetrix Proprietary Database