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[192306_s_at] Eukaryotic protein kinase domain

Description K09B11.5 /REP_DB=WormBase Gene ID /WP=CE19992 /TR=Q9U3B5 /GB=CAB63235.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=Eukaryotic protein kinase domain
Cluster 6grp_total > 77      4grp_total > 25      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 3.9
(-3)
A 		8.3
(1.6)
A 		8.1
(1.4)
A 		9.6
(3)
A 		6
B 6.3
(3)
A 		5.2
(-3)
A 		5.7
(-0.3)
A 		5.7
(-0.3)
A 		1
C 2.5
(-3)
A 		8.3
(-0.5)
A 		6.1
(-1.5)
A 		16.6
(3)
A 		14
avr 4.2
(-3) 		7.3
(-0.1) 		6.6
(-0.8) 		10.6
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 71
1 2 3 4 5 6 range
A 10.3
(0.2)
A 		6.5
(-1.4)
A 		2.8
(-3)
A 		5.6
(-1.8)
A 		10.7
(0.4)
A 		16.7
(3)
A 		14
B 5.1
(-3)
A 		5
(-3)
A 		5.6
(-2.9)
A 		7.8
(-2.8)
A 		18.4
(-1.8)
A 		73.5
(3)
A 		69
C 2.9
(-3)
A 		6.6
(-1.9)
A 		3.7
(-2.8)
A 		7.8
(-1.6)
A 		13.2
(0)
A 		23.3
(3)
A 		20
avr 6.1
(-2.6) 		6
(-2.6) 		4
(-3) 		7.1
(-2.4) 		14.1
(-1.2) 		37.8
(3) 		34
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2
1 2 3 4 range
A 1.4
(-3)
A 		1.8
(0.4)
A 		2.1
(3)
A 		1.4
(-3)
A 		1
B 2
(3)
A 		1.3
(-2.3)
A 		1.3
(-2.3)
A 		1.2
(-3)
A 		1
C 0.8
(-3)
A 		2.4
(1.8)
A 		2.1
(0.9)
A 		2.8
(3)
A 		2
avr 1.4
(-3) 		1.8
(3) 		1.8
(3) 		1.8
(3) 		0

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 27
1 2 3 4 5 6 range
A 1.9
(-0.8)
A 		2.3
(-0.1)
A 		0.6
(-3)
A 		1.4
(-1.6)
A 		3.2
(1.5)
A 		4.1
(3)
A 		4
B 1.8
(-2.9)
A 		1.7
(-2.9)
A 		1.4
(-3)
A 		2.1
(-2.8)
A 		7.5
(-1.6)
A 		27.4
(3)
A 		26
C 1.1
(-2.8)
A 		2
(-2)
A 		0.9
(-3)
A 		1.6
(-2.4)
A 		4.3
(-0)
A 		7.8
(3)
A 		7
avr 1.6
(-2.7) 		2
(-2.5) 		1
(-3) 		1.7
(-2.7) 		5
(-1) 		13.1
(3) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192306_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9719
Sequence Type Exemplar sequence
Representative Public ID K09B11.5 NCBI
Target Description K09B11.5 /REP_DB=WormBase Gene ID /WP=CE19992 /TR=Q9U3B5 /GB=CAB63235.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=Eukaryotic protein kinase domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070190(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070190 NCBI Caenorhabditis elegans K09B11.5 (K09B11.5) mRNA, complete cds. 11/11 A 
SNAP00000037692 ENSEMBL cdna:SNAP chromosome:CEL140:IV:13431257:13433973:-1 11/11 None
GENEFINDER00000037713 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:13430740:13436401:-1 11/11 None
K09B11.5 ENSEMBL cdna:known chromosome:CEL140:IV:13431257:13433973:-1 gene:K09B11.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:13339698-13342308(+) 93.5 93.5
chrIV:13431262-13433979(-) 100.0 100.0
Public Domain and Genome References
Gene Title Eukaryotic protein kinase domain
Ensembl K09B11.5
Entrez Gene 178307 Entrez gene
SwissProt Q9U3B5 EMBL-EBI
WormBase CE19992 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB63235 Hypothetical protein K09B11.5 [Caenorhabditis elegans] ref|NP_502591.1| K09B11.5 [Caenorhabditis elegans] 0.0
blast T26078 hypothetical protein W02A2.4 - Caenorhabditis elegans 0.0
blast CAE71276 Hypothetical protein CBG18159 [Caenorhabditis briggsae] 1.0E-169
Protein Families
Method ID Description E-Value
ec ABL1_HUMAN (P00519) Proto-oncogene tyrosine-protein kinase ABL1 (EC 2.7.1.112) (p150) (c-ABL) 1.0E-107
ec ABL1_HUMAN (P00519) Proto-oncogene tyrosine-protein kinase ABL1 (EC 2.7.1.112) (p150) (c-ABL) 1.0E-119
hanks 2.1.11 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; CMKI 2.0E-60
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.3E-53
Pfam IPR000719 EMBL-EBI Protein kinase 1.3E-53
Sequence
Target Sequence 
>C. ELEGANS:192306_S_AT
aagaataccatgtgtcgtgcaaactttcaaaatgccaagcgtcgtctcaaaattgtgatt
gaccagtacgaccatccagacggccacaaattggaattgaaaaaatccgaggagcagaaa
aggctgaccattcgcagatccacagccactgtgacccgaaaaaaatctcgagatggagga
cgtcaagcatctgtcgaacggaaggggcacggtactcaacgaaaagtggtctcagttctt
cgaaaagataaaggggctctgaagcttccgtctggaatcgccacgaaccttgagagaccg
tctccgccatcgcacactgcaaaatcgcatactgttaaatcgccgatgactcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGAATACCATGTGTCGTGCAAACT 575 151 1276 Antisense
ACCATGTGTCGTGCAAACTTTCAAA 149 87 1282 Antisense
GGAGCAGAAAAGGCTGACCATTCGC 32 521 1386 Antisense
TCGCAGATCCACAGCCACTGTGACC 113 601 1407 Antisense
CACAGCCACTGTGACCCGAAAAAAA 285 181 1416 Antisense
CAACGAAAAGTGGTCTCAGTTCTTC 527 185 1492 Antisense
GGAATCGCCACGAACCTTGAGAGAC 200 533 1549 Antisense
CACGAACCTTGAGAGACCGTCTCCG 233 195 1557 Antisense
CGCCATCGCACACTGCAAAATCGCA 420 257 1580 Antisense
AAAATCGCATACTGTTAAATCGCCG 639 127 1596 Antisense
TACTGTTAAATCGCCGATGACTCCA 321 645 1605 Antisense
Sequence Source Affymetrix Proprietary Database