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[192304_s_at] Protein kinase C terminal domain

Description T20F10.1 /REP_DB=WormBase Gene ID /WP=CE13844 /TR=O45797 /GB=CAB04745.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Protein kinase C terminal domain
Cluster 6grp_highest_10_10 > 33      6grp_high1_10_10 > 32      4grp_highest_8_8 > 53      4grp_high_8_8 > 09      6grp_highest_nonorm > 61      6grp_total > 31      4grp_total > 23      6grp_highest_sub > 02      6grp_cgc > 62      6grp_high1_sub > 02_03      6grp_cgc_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 277
1 2 3 4 range
A 492.1
(3)
P 		381.6
(-0)
P 		271.7
(-3)
P 		429.7
(1.3)
P 		220
B 407.2
(1.2)
P 		245.3
(-2.4)
P 		216.4
(-3)
P 		487.8
(3)
P 		271
C 493.3
(3)
P 		230.1
(-3)
P 		330.2
(-0.7)
P 		352.1
(-0.2)
P 		263
avr 464.2
(3) 		285.7
(-2.6) 		272.8
(-3) 		423.2
(1.7) 		191

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 326
1 2 3 4 5 6 range
A 374.6
(-1.8)
P 		519.7
(2.3)
P 		543.7
(3)
P 		347
(-2.6)
P 		440.5
(0.1)
P 		333.1
(-3)
P 		211
B 568.5
(2.6)
P 		583.4
(3)
P 		557.9
(2.4)
P 		475.3
(0.3)
P 		346.8
(-3)
P 		347.9
(-3)
P 		237
C 395.3
(0.9)
P 		471.9
(3)
P 		431.9
(1.9)
P 		460.7
(2.7)
P 		356.6
(-0.2)
P 		257.9
(-3)
P 		214
avr 446.1
(0.8) 		525
(3) 		511.2
(2.6) 		427.7
(0.2) 		381.3
(-1.1) 		313
(-3) 		212
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 129
1 2 3 4 range
A 179.2
(3)
P 		82.8
(-2)
P 		71
(-2.6)
P 		62.9
(-3)
P 		116
B 129.8
(3)
P 		60.6
(-2.2)
P 		50.1
(-3)
P 		103.9
(1.1)
P 		80
C 163.3
(3)
P 		66.2
(-2.6)
P 		115.1
(0.2)
P 		59.4
(-3)
P 		104
avr 157.4
(3) 		69.9
(-3) 		78.7
(-2.4) 		75.4
(-2.6) 		88

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 129
1 2 3 4 5 6 range
A 69.1
(-3)
P 		184.8
(3)
P 		118.4
(-0.4)
P 		88
(-2)
P 		132.9
(0.3)
P 		81.1
(-2.4)
P 		116
B 197.8
(3)
P 		194.2
(2.7)
P 		135.5
(-2.2)
P 		125.9
(-3)
P 		140.9
(-1.7)
P 		129.7
(-2.7)
P 		72
C 146.5
(3)
P 		144
(2.8)
P 		109.9
(-0.6)
P 		92.6
(-2.4)
P 		115.4
(-0.1)
P 		86.3
(-3)
P 		60
avr 137.8
(0.1) 		174.3
(3) 		121.3
(-1.2) 		102.2
(-2.7) 		129.7
(-0.6) 		99
(-3) 		75
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192304_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1364
Sequence Type Exemplar sequence
Representative Public ID T20F10.1 NCBI
Target Description T20F10.1 /REP_DB=WormBase Gene ID /WP=CE13844 /TR=O45797 /GB=CAB04745.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Protein kinase C terminal domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060298(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060298 NCBI Caenorhabditis elegans T20F10.1 (T20F10.1) mRNA, complete cds. 11/11 None
SNAP00000031261 ENSEMBL cdna:SNAP chromosome:CEL140:I:10300261:10305968:-1 11/11 None
GENEFINDER00000031265 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:10300261:10305968:-1 11/11 None
T20F10.1 ENSEMBL cdna:known chromosome:CEL140:I:10300016:10306469:-1 gene:T20F10.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:10300191-10305899(-) 100.0 100.0
Public Domain and Genome References
Gene Title Protein kinase C terminal domain
Ensembl T20F10.1
Entrez Gene 172896 Entrez gene
SwissProt O45797 EMBL-EBI
WormBase CE13844 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1605384_AT LATS, large tumor suppressor, homolog 1 (Drosophila) cfa
CANINE_2:CFA.2347.1.S1_AT LATS, large tumor suppressor, homolog 1 (Drosophila) cfa
CANINE_2:CFAAFFX.1540.1.S1_AT LATS, large tumor suppressor, homolog 1 (Drosophila) cfa
DROSOPHILA_2:1635182_AT warts dm
DROSGENOME1:143684_AT warts dm
CHICKEN:GGA.8389.1.S1_AT similar to LATS homolog 1; LATS (large tumor suppressor, Drosophila) homolog 1 gga
HG-U133_PLUS_2:219813_AT LATS, large tumor suppressor, homolog 1 (Drosophila) hs
HG-U133A:219813_AT LATS, large tumor suppressor, homolog 1 (Drosophila) hs
HG-U133A_2:219813_AT LATS, large tumor suppressor, homolog 1 (Drosophila) hs
HU35KSUBA:HUM_ALU_AT LATS, large tumor suppressor, homolog 1 (Drosophila) hs
HU35KSUBB:HUM_ALU_AT LATS, large tumor suppressor, homolog 1 (Drosophila) hs
HU35KSUBC:HUM_ALU_AT LATS, large tumor suppressor, homolog 1 (Drosophila) hs
HU35KSUBD:HUM_ALU_AT LATS, large tumor suppressor, homolog 1 (Drosophila) hs
HUGENEFL:HUM_ALU_AT LATS, large tumor suppressor, homolog 1 (Drosophila) hs
HC-G110:HUM_ALU_AT LATS, large tumor suppressor, homolog 1 (Drosophila) hs
U133_X3P:G10862687_3P_X_AT LATS, large tumor suppressor, homolog 1 (Drosophila) hs
U133_X3P:G10862687_3P_AT LATS, large tumor suppressor, homolog 1 (Drosophila) hs
U133_X3P:HS2.250691.2.A1_3P_S_AT LATS, large tumor suppressor, homolog 1 (Drosophila) hs
U133_X3P:HS2.250691.1.A1_3P_AT LATS, large tumor suppressor, homolog 1 (Drosophila) hs
HG-U133_PLUS_2:1570231_AT LATS, large tumor suppressor, homolog 1 (Drosophila) hs
HG-U133_PLUS_2:1570425_S_AT LATS, large tumor suppressor, homolog 1 (Drosophila) hs
HG-U95D:70095_AT LATS, large tumor suppressor, homolog 1 (Drosophila) hs
HG-U95B:46129_AT LATS, large tumor suppressor, homolog 1 (Drosophila) hs
HG-U95B:59159_R_AT LATS, large tumor suppressor, homolog 1 (Drosophila) hs
HU35KSUBD:RC_R97818_AT LATS, large tumor suppressor, homolog 1 (Drosophila) hs
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04745 Hypothetical protein T20F10.1 [Caenorhabditis elegans] ref|NP_492699.1| T20F10.1 [Caenorhabditis elegans] 0.0
blast CAE73788 Hypothetical protein CBG21338 [Caenorhabditis briggsae] 0.0
blast AAF57085 CG12072-PA [Drosophila melanogaster] ref|NP_733403.1| CG12072-PA [Drosophila melanogaster] 1.0E-121
Protein Families
Method ID Description E-Value
hanks 1.9.4 AGC group; AGC Other; cot-1 6.0E-80
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.2E-68
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 4.6E-5
Sequence
Target Sequence 
>C. ELEGANS:192304_S_AT
ggagaaacttccttgattttacatattgtggaaatctatcaaaagagtgccttatgatga
ttcaacaactaatttgtgacgcgtcgtcaagattgggatctcacgggaaggatgttgccg
aacgaacggcacaagtcaaaaatcatccatggttcaggggaattgactgggttaacttga
gaaaactgagagctgattatatttatattcctcgggtcactcacgatgaagacacttcta
attttgaaacgtttcaagacaatgaccgtgctgacaagccgaatgtgcgaggtcttcata
atccagccttctatgaattcacttatcgtcatttctttgatacggattctgttggttgcc
catcacttcgaccatctcgtcgtcggtcacttcggccattacttgaaaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAGAAACTTCCTTGATTTTACATA 349 517 2279 Antisense
GACGCGTCGTCAAGATTGGGATCTC 383 381 2356 Antisense
GGGATCTCACGGGAAGGATGTTGCC 60 497 2373 Antisense
TATTCCTCGGGTCACTCACGATGAA 451 661 2484 Antisense
GACAATGACCGTGCTGACAAGCCGA 103 365 2536 Antisense
ACAAGCCGAATGTGCGAGGTCTTCA 148 111 2552 Antisense
CGAGGTCTTCATAATCCAGCCTTCT 343 249 2566 Antisense
ATCCAGCCTTCTATGAATTCACTTA 647 39 2579 Antisense
GAATTCACTTATCGTCATTTCTTTG 173 325 2593 Antisense
TACGGATTCTGTTGGTTGCCCATCA 102 649 2619 Antisense
GTCACTTCGGCCATTACTTGAAAAC 577 463 2664 Antisense
Sequence Source Affymetrix Proprietary Database