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[192303_s_at]

Description M03D4.1B /REP_DB=WormBase Gene ID /WP=CE12346 /GEN=zen-4 /TR=Q21492 /GB=AAC24407.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=kinesin-like protein
Cluster 6grp_total > 78      4grp_total > 53      6grp_cgc > 49      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 398
1 2 3 4 range
A 479.7
(3)
A 		435.8
(2)
A 		471.7
(2.8)
A 		222
(-3)
A 		258
B 516.6
(3)
A 		443.5
(1.8)
A 		391.8
(0.9)
A 		164.2
(-3)
A 		352
C 562.6
(3)
A 		420.7
(0.6)
A 		382.9
(-0)
A 		203.4
(-3)
A 		359
avr 519.6
(3) 		433.3
(1.4) 		415.5
(1.1) 		196.5
(-3) 		323

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1171
1 2 3 4 5 6 range
A 421.9
(-2.5)
A 		398.4
(-2.6)
A 		321.2
(-3)
A 		378
(-2.7)
A 		561
(-1.7)
P 		1426.9
(3)
P 		1106
B 371.4
(-2.3)
A 		324.3
(-2.6)
A 		255.6
(-3)
A 		385.6
(-2.2)
A 		490.8
(-1.5)
P 		1195.2
(3)
P 		940
C 421.5
(-2.4)
A 		339.1
(-2.9)
A 		330.1
(-3)
A 		395.1
(-2.6)
A 		812.5
(0.2)
M 		1229.3
(3)
P 		899
avr 404.9
(-2.4) 		353.9
(-2.7) 		302.3
(-3) 		386.2
(-2.5) 		621.4
(-1) 		1283.8
(3) 		982
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 154
1 2 3 4 range
A 174.7
(3)
A 		94.6
(-0.4)
A 		123.3
(0.8)
A 		32.5
(-3)
A 		142
B 164.7
(3)
A 		109.5
(0.4)
A 		90.8
(-0.4)
A 		35
(-3)
A 		130
C 186.2
(3)
A 		121
(0.4)
A 		133.5
(0.9)
A 		34.3
(-3)
A 		152
avr 175.2
(3) 		108.4
(0.2) 		115.9
(0.5) 		33.9
(-3) 		141

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 384
1 2 3 4 5 6 range
A 77.8
(-2.8)
A 		141.7
(-1.4)
A 		70
(-3)
A 		95.9
(-2.4)
A 		169.3
(-0.9)
P 		347.4
(3)
P 		277
B 129.2
(-2)
A 		108
(-2.3)
A 		62.1
(-3)
A 		102.1
(-2.4)
A 		199.4
(-0.9)
P 		445.7
(3)
P 		384
C 156.2
(-1.6)
A 		103.5
(-2.6)
A 		84
(-2.9)
A 		79.5
(-3)
A 		262.9
(0.3)
M 		411.5
(3)
P 		332
avr 121.1
(-2.1) 		117.7
(-2.2) 		72
(-3) 		92.5
(-2.6) 		210.5
(-0.5) 		401.5
(3) 		330
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192303_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10850
Sequence Type Exemplar sequence
Representative Public ID M03D4.1B NCBI
Target Description M03D4.1B /REP_DB=WormBase Gene ID /WP=CE12346 /GEN=zen-4 /TR=Q21492 /GB=AAC24407.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=kinesin-like protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028140(11), NM_001028138(11), NM_001028139(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028140 NCBI Caenorhabditis elegans Zygotic epidermal ENclosure defective family member (zen-4) (zen-4) mRNA, complete cds. 11/11 None
NM_001028138 NCBI Caenorhabditis elegans Zygotic epidermal ENclosure defective family member (zen-4) (zen-4) mRNA, complete cds. 11/11 None
NM_001028139 NCBI Caenorhabditis elegans Zygotic epidermal ENclosure defective family member (zen-4) (zen-4) mRNA, complete cds. 11/11 None
SNAP00000036222 ENSEMBL cdna:SNAP chromosome:CEL140:IV:6118208:6121420:1 10/11 None
GENEFINDER00000036229 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:6118208:6121420:1 11/11 None
M03D4.1a ENSEMBL cdna:known chromosome:CEL140:IV:6118208:6121420:1 gene:M03D4.1 11/11 None
M03D4.1c ENSEMBL cdna:known chromosome:CEL140:IV:6118208:6121745:1 gene:M03D4.1 11/11 A 
M03D4.1b ENSEMBL cdna:known chromosome:CEL140:IV:6118208:6120827:1 gene:M03D4.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177374 4 3 NM_001028138 192303_s_at (11)
NM_001028139 192303_s_at (11)
NM_001028140 174527_at (10), 192303_s_at (11)
NM_001028141 174527_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:6118213-6120833(+) 100.0 100.0
Public Domain and Genome References
Ensembl M03D4.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM97993 Zygotic epidermal enclosure defective protein 4, isoform c [Caenorhabditis elegans] ref|NP_001023311.1| Zygotic epidermal ENclosure defective family member (zen-4) [Caenorhabditis elegans] 0.0
blast AAC24406 Zygotic epidermal enclosure defective protein 4, isoform a [Caenorhabditis elegans] ref|NP_001023309.1| Zygotic epidermal ENclosure defective family member (zen-4) [Caenorhabditis elegans] gb|AAC33437.1| kinesin-like protein ZEN-4a [Caenorhabditis elegans] 0.0
blast AAC24407 Zygotic epidermal enclosure defective protein 4, isoform b [Caenorhabditis elegans] ref|NP_001023310.1| Zygotic epidermal ENclosure defective family member (zen-4) [Caenorhabditis elegans] gb|AAC33438.1| kinesin-like protein ZEN-4b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
scop a.2.11.Fe,Mn superoxide dismutase (SOD), N-terminal domain All alpha proteins; Long alpha-hairpin; Fe,Mn superoxide dismutase (SOD), N-terminal domain; Fe,Mn superoxide dismutase (SOD), N-terminal domain 1.39999997615814
Pfam IPR001752 EMBL-EBI Kinesin, motor region 1.0E-126
Pfam IPR001752 EMBL-EBI Kinesin, motor region 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:192303_S_AT
aattattgataatccatccccgtctgttgcttctcttcgatcacgatttgatcaagagaa
tgtggctcatccaacagctccaatccaaactccaccaccgccatatcaaactccaggacg
tgctccagtcttcaaaaaacggttagaagccactacatcgaccactgtaatgtctggatc
ttcaagtggaggaagtggtcaacagggttacgttaatccaaaatatcaaagaagatccaa
gtctgcatctcgtctattggatcatcagccacttcatcgagttccaacaggaacggttct
tcagtctcgtacaccggctaacgccatacggactactaaacctgagatgcatcagttgaa
caaatccggagaataccgtctcacgcat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATTATTGATAATCCATCCCCGTCT 160 175 1815 Antisense
TTGCTTCTCTTCGATCACGATTTGA 675 703 1841 Antisense
AGAATGTGGCTCATCCAACAGCTCC 225 75 1871 Antisense
ACCGCCATATCAAACTCCAGGACGT 457 89 1911 Antisense
GGACGTGCTCCAGTCTTCAAAAAAC 111 525 1930 Antisense
AGCCACTACATCGACCACTGTAATG 310 83 1962 Antisense
GAAGATCCAAGTCTGCATCTCGTCT 112 339 2045 Antisense
CTATTGGATCATCAGCCACTTCATC 478 223 2068 Antisense
CAGGAACGGTTCTTCAGTCTCGTAC 322 209 2102 Antisense
GGCTAACGCCATACGGACTACTAAA 90 537 2130 Antisense
ATCCGGAGAATACCGTCTCACGCAT 517 39 2178 Antisense
Sequence Source Affymetrix Proprietary Database