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[192302_at] heparan sulfate sulfotransferase

Description F40H3.5 /REP_DB=WormBase Gene ID /WP=CE19866 /TR=Q9TZK5 /GB=AAC67432.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=heparan sulfate sulfotransferase
Cluster 6grp_highest_10_10 > 34      6grp_high1_10_10 > 43      4grp_highest_8_8 > 31      4grp_high_8_8 > 13      6grp_highest_nonorm > 80      6grp_total > 30      4grp_total > 11      6grp_highest_sub > 02_03      6grp_cgc > 72      6grp_high1_sub > 02      6grp_cgc_sub > 02      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 207
1 2 3 4 range
A 148.3
(-0)
P 		163.8
(0.7)
P 		85.1
(-3)
P 		212.3
(3)
P 		127
B 163.5
(-2.4)
P 		153.7
(-3)
P 		169.9
(-2)
P 		248.3
(3)
P 		95
C 181.3
(-2.4)
P 		175.9
(-2.6)
P 		168.7
(-3)
P 		291.9
(3)
P 		123
avr 164.4
(-1.7) 		164.5
(-1.7) 		141.2
(-3) 		250.8
(3) 		110

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 184
1 2 3 4 5 6 range
A 195.7
(-0.4)
P 		243.2
(1.3)
P 		293.1
(3)
P 		241.5
(1.2)
P 		190.7
(-0.6)
P 		120.2
(-3)
P 		173
B 191.5
(-1.4)
P 		294.2
(3)
P 		262.7
(1.6)
P 		245
(0.9)
P 		162.2
(-2.7)
P 		155
(-3)
P 		139
C 167.7
(-0.8)
P 		267.7
(3)
P 		160.7
(-1.1)
P 		242.5
(2)
P 		110.3
(-3)
P 		183.2
(-0.2)
P 		157
avr 185
(-1.3) 		268.4
(3) 		238.8
(1.5) 		243
(1.7) 		154.4
(-2.9) 		152.8
(-3) 		116
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 38
1 2 3 4 range
A 54
(3)
P 		35.6
(-0.5)
P 		22.2
(-3)
P 		31.1
(-1.3)
P 		32
B 52.1
(2.7)
P 		38
(-3)
P 		39.4
(-2.4)
P 		52.9
(3)
P 		15
C 60
(3)
P 		50.6
(-2.2)
P 		58.8
(2.3)
P 		49.2
(-3)
P 		11
avr 55.4
(3) 		41.4
(-2.5) 		40.1
(-3) 		44.4
(-1.3) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 69
1 2 3 4 5 6 range
A 36.1
(-2.3)
P 		86.5
(3)
P 		63.8
(0.6)
P 		61.2
(0.3)
P 		57.5
(-0)
P 		29.3
(-3)
P 		57
B 66.6
(-1.7)
P 		97.9
(3)
P 		63.8
(-2.1)
P 		64.9
(-1.9)
P 		65.9
(-1.8)
P 		57.8
(-3)
P 		40
C 62.1
(0.4)
P 		81.7
(3)
P 		40.9
(-2.3)
P 		48.8
(-1.3)
P 		35.7
(-3)
P 		61.3
(0.3)
P 		46
avr 54.9
(-2.2) 		88.7
(3) 		56.2
(-2) 		58.3
(-1.7) 		53
(-2.5) 		49.5
(-3) 		39
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192302_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5590
Sequence Type Exemplar sequence
Representative Public ID F40H3.5 NCBI
Target Description F40H3.5 /REP_DB=WormBase Gene ID /WP=CE19866 /TR=Q9TZK5 /GB=AAC67432.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=heparan sulfate sulfotransferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062829(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062829 NCBI Caenorhabditis elegans Heparan SulphoTransferase family member (hst-3) (hst-3) mRNA, complete cds. 11/11 None
SNAP00000001568 ENSEMBL cdna:SNAP chromosome:CEL140:II:6167811:6169184:-1 11/11 None
GENEFINDER00000001578 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6167785:6169184:-1 11/11 None
F40H3.5 ENSEMBL cdna:known chromosome:CEL140:II:6167742:6169184:-1 gene:F40H3.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6167738-6169181(-) 100.0 100.0
Public Domain and Genome References
Gene Title heparan sulfate sulfotransferase
Gene Symbol hst-3
Ensembl F40H3.5
Entrez Gene 185573 Entrez gene
SwissProt Q9TZK5 EMBL-EBI
WormBase CE19866 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CHICKEN:GGA.16492.1.S1_AT similar to heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1 precursor; heparin-glucosamine 3-O-sulfotransferase gga
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
8146 sulfotransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC67432 Heparan sulphotransferase protein 3 [Caenorhabditis elegans] ref|NP_495230.1| Heparan SulphoTransferase family member (hst-3) [Caenorhabditis elegans] 1.0E-161
blast AAC67432 Heparan sulphotransferase protein 3 [Caenorhabditis elegans] ref|NP_495230.1| Heparan SulphoTransferase family member (hst-3) [Caenorhabditis elegans] 1.0E-158
blast CAE59255 Hypothetical protein CBG02583 [Caenorhabditis briggsae] 1.0E-136
blast CAE59255 Hypothetical protein CBG02583 [Caenorhabditis briggsae] 1.0E-135
blast XP_421692 PREDICTED: similar to heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1 precursor; heparin-glucosamine 3-O-sulfotransferase [Gallus gallus] 3.0E-52
blast XP_421692 PREDICTED: similar to heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1 precursor; heparin-glucosamine 3-O-sulfotransferase [Gallus gallus] 1.0E-51
Protein Domains
Database ID Description E-Value
Pfam IPR000863 EMBL-EBI Sulfotransferase 3.4E-16
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495230.1 1 7-26
F40H3.5 1 7-26
SNAP00000001568 1 7-26
GENEFINDER00000001578 1 7-26
Sequence
Target Sequence 
>C. ELEGANS:192302_AT
atcgctcttcacccaaaagttcgaattgtgagacgagaaactcatttctttgattcaaat
tacacacttggatttgattggtatcgagatcaaatgcctgaagtcgaaaatgataacgaa
attgtcatcgaaaaaacgccagcgtacttcacaaatgagcatgttcccaaaagagtttac
gaaatgaatccagatatgaaacttattttaattgttcgacatcctgtatatagaactgtt
agtgacttcacacaggtatactacaacaaattggaacaaaataaaacacttccagtgcta
tcagtggaagcatttaaaacgaatgaagctggaattgagaaaatcaatatggagtataaa
ccaatgacaaattctctttacgatgttcatatttcaaaatggctcaagtactttgatctc
aaaaattttctttttgtgaatggagatgttttcagagcaaacccccttcgtgagttacgg
aaagtggaagaattcttaggattagagagatcgattaccccgagccaattggtttttgat
tacaataagggattcttttgctttcga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCGCTCTTCACCCAAAAGTTCGAA 216 37 220 Antisense
ACGCCAGCGTACTTCACAAATGAGC 504 91 355 Antisense
GAGCATGTTCCCAAAAGAGTTTACG 566 389 376 Antisense
AATTGTTCGACATCCTGTATATAGA 491 179 429 Antisense
GTGACTTCACACAGGTATACTACAA 151 483 461 Antisense
AAACACTTCCAGTGCTATCAGTGGA 360 129 503 Antisense
GACAAATTCTCTTTACGATGTTCAT 618 365 585 Antisense
AATGGCTCAAGTACTTTGATCTCAA 112 163 617 Antisense
AGATGTTTTCAGAGCAAACCCCCTT 362 65 663 Antisense
TAGAGAGATCGATTACCCCGAGCCA 205 653 722 Antisense
CAATAAGGGATTCTTTTGCTTTCGA 425 171 762 Antisense
Sequence Source Affymetrix Proprietary Database