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[192281_at] Long-chain-fatty-acid coA ligase

Description F41C3.3 /REP_DB=WormBase Gene ID /WP=CE02731 /TR=Q20264 /GB=AAC46810.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Long-chain-fatty-acid coA ligase
Cluster 6grp_highest_10_10 > 31      6grp_high1_10_10 > 04      4grp_highest_8_8 > 43      4grp_high_8_8 > 24      6grp_highest_nonorm > 51      6grp_total > 24      4grp_total > 47      6grp_highest_sub > De      6grp_cgc > 32      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 452
1 2 3 4 range
A 575.5
(3)
P 		168.9
(-3)
P 		313.9
(-0.9)
P 		232.4
(-2.1)
P 		407
B 362.5
(3)
P 		158.9
(-3)
P 		185.1
(-2.2)
P 		228.8
(-0.9)
P 		204
C 610.5
(3)
P 		222.4
(-3)
P 		226
(-2.9)
P 		261.4
(-2.4)
P 		388
avr 516.2
(3) 		183.4
(-3) 		241.7
(-1.9) 		240.9
(-2) 		333

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 851
1 2 3 4 5 6 range
A 277
(-3)
P 		773.4
(3)
P 		502.8
(-0.3)
P 		689.4
(2)
P 		327.6
(-2.4)
P 		417.6
(-1.3)
P 		496
B 835.1
(3)
P 		676.9
(1)
P 		518.6
(-1.1)
P 		371
(-3)
P 		577.3
(-0.3)
P 		458.2
(-1.9)
P 		464
C 1127.9
(3)
P 		733.6
(-0.1)
P 		783.5
(0.3)
P 		417.3
(-2.5)
P 		564.7
(-1.4)
P 		356
(-3)
P 		772
avr 746.7
(3) 		728
(2.7) 		601.6
(0.4) 		492.6
(-1.5) 		489.9
(-1.6) 		410.6
(-3) 		336
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 176
1 2 3 4 range
A 209.6
(3)
P 		36.7
(-2.9)
P 		82.1
(-1.4)
P 		34
(-3)
P 		176
B 115.6
(3)
P 		39.3
(-3)
P 		42.9
(-2.7)
P 		48.8
(-2.3)
P 		76
C 202.1
(3)
P 		64
(-2.2)
P 		78.8
(-1.7)
P 		44.1
(-3)
P 		158
avr 175.8
(3) 		46.7
(-2.8) 		67.9
(-1.8) 		42.3
(-3) 		134

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 367
1 2 3 4 5 6 range
A 51.1
(-3)
P 		275.1
(3)
P 		109.5
(-1.4)
P 		174.8
(0.3)
P 		98.9
(-1.7)
P 		101.7
(-1.6)
P 		224
B 290.6
(3)
P 		225.3
(1)
P 		125.9
(-2.1)
P 		98.3
(-3)
P 		234.5
(1.2)
P 		170.9
(-0.7)
P 		192
C 418.1
(3)
P 		223.9
(-0.5)
P 		199.4
(-0.9)
P 		83.9
(-3)
P 		182.7
(-1.2)
P 		119.2
(-2.4)
P 		334
avr 253.3
(3) 		241.4
(2.5) 		144.9
(-1.8) 		119
(-3) 		172
(-0.6) 		130.6
(-2.5) 		134
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192281_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4790
Sequence Type Exemplar sequence
Representative Public ID F41C3.3 NCBI
Target Description F41C3.3 /REP_DB=WormBase Gene ID /WP=CE02731 /TR=Q20264 /GB=AAC46810.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Long-chain-fatty-acid coA ligase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062447
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
SNAP00000035622 ENSEMBL cdna:SNAP chromosome:CEL140:II:4742125:4745124:1 None
NM_062447 NCBI Caenorhabditis elegans F41C3.3 (F41C3.3) mRNA, complete cds. None
GENEFINDER00000035626 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4742125:4745124:1 None
F41C3.3.2 ENSEMBL cdna:known chromosome:CEL140:II:4742114:4745267:1 gene:F41C3.3 None
F41C3.3.1 ENSEMBL cdna:known chromosome:CEL140:II:4742123:4745267:1 gene:F41C3.3 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4741325-4745123(+) 97.06 100.0
Public Domain and Genome References
Gene Title Long-chain-fatty-acid coA ligase
Gene Symbol acs-11
Ensembl F41C3.3
Entrez Gene 173820 Entrez gene
SwissProt Q20264 EMBL-EBI
WormBase CE32658 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC46810 Hypothetical protein F41C3.3 [Caenorhabditis elegans] ref|NP_494848.3| F41C3.3 [Caenorhabditis elegans] 0.0
blast CAE73220 Hypothetical protein CBG20626 [Caenorhabditis briggsae] 0.0
blast NP_503845 C50H11.1 [Caenorhabditis elegans] gb|AAG23991.1| Hypothetical protein C50H11.1 [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec AC2L_HUMAN (Q9NUB1) Acetyl-coenzyme A synthetase 2-like, mitochondrial precursor (EC 6.2.1.1) (Acetate--CoA ligase 2) (Acetyl-CoA synthetase 2) 1.0E-109
ec 4CL1_ARATH (Q42524) 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1) 1.0E-134
ec 4CL1_ARATH (Q42524) 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1) 1.0E-139
Protein Domains
Database ID Description E-Value
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 2.2E-105
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000035626 1 522-544
Sequence
Target Sequence 
>C. ELEGANS:192281_AT
ggagttatgactactaatccattgaatggagagagaaaagctggaactgttggcccggct
gtacaaggagttggatgtagaattgcaaaaaatggaggaattgaagtgaagacaaatgcg
attttcgctggatattggaagaatccaaagaagacagctgaagaattcactgaagatgga
tggtttaaaactggagatgttggacatcttgatgaggatggttatttgacgattggtgga
agatcaaaggatatggttattactggaggattaaatgtatatccaaaagaacttgaagat
ttcattgatacacttccatttgttaaagaatctgcagttattgcatctccacatccagat
tttggagaagcagttgttgcaattgtagtcccgtcggagaaggtgactgatgagaaggag
tttgagaagaagcttattggaattatgaagaagaaggttgccaattataaggcaattttc
aaatttccatcggacaccgcaattctcgttcagatatttgttccaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAGTTATGACTACTAATCCATTGA 531 513 1090 Antisense
ACTGTTGGCCCGGCTGTACAAGGAG 167 97 1135 Antisense
GTGAAGACAAATGCGATTTTCGCTG 559 481 1195 Antisense
GCGATTTTCGCTGGATATTGGAAGA 699 289 1207 Antisense
GAGGATGGTTATTTGACGATTGGTG 577 403 1303 Antisense
GATTTCATTGATACACTTCCATTTG 588 427 1387 Antisense
GAATCTGCAGTTATTGCATCTCCAC 349 329 1417 Antisense
TTGCATCTCCACATCCAGATTTTGG 686 703 1430 Antisense
TTCAAATTTCCATCGGACACCGCAA 38 685 1567 Antisense
ACACCGCAATTCTCGTTCAGATATT 417 109 1583 Antisense
TTCTCGTTCAGATATTTGTTCCAAA 225 693 1592 Antisense
Sequence Source Affymetrix Proprietary Database