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[192278_s_at] endothelin converting enzyme 1

Description T16A9.4 /REP_DB=WormBase Gene ID /WP=CE18259 /TR=SW:Q22523 /GB=CAB00879.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=endothelin converting enzyme 1
Cluster 6grp_highest_10_10 > 42      6grp_high1_10_10 > 03      4grp_highest_8_8 > 54      4grp_high_8_8 > 10      6grp_highest_nonorm > 50      6grp_total > 25      4grp_total > 22      6grp_highest_sub > 02      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 360
1 2 3 4 range
A 677.5
(3)
P 		429.4
(-1.2)
P 		319.9
(-3)
P 		539.2
(0.7)
P 		358
B 591
(1.4)
P 		415.7
(-1.8)
P 		349.3
(-3)
P 		679.8
(3)
P 		331
C 638.1
(2.4)
P 		367.7
(-2.9)
P 		362.7
(-3)
P 		669.8
(3)
P 		307
avr 635.5
(3) 		404.3
(-1.8) 		344
(-3) 		629.6
(2.9) 		292

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 373
1 2 3 4 5 6 range
A 551.3
(2.2)
P 		586.2
(3)
P 		473.1
(0.4)
P 		376.5
(-1.8)
P 		324.8
(-3)
P 		378.3
(-1.8)
P 		261
B 684.9
(3)
P 		602.9
(1.7)
P 		477.7
(-0.3)
P 		407.1
(-1.5)
P 		311.8
(-3)
P 		407.6
(-1.5)
P 		373
C 537.5
(1.5)
P 		585
(3)
P 		456.3
(-0.9)
P 		474.3
(-0.4)
P 		389
(-3)
P 		393.3
(-2.9)
P 		196
avr 591.2
(3) 		591.4
(3) 		469
(0.1) 		419.3
(-1.1) 		341.9
(-3) 		393.1
(-1.8) 		250
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 168
1 2 3 4 range
A 246.7
(3)
P 		93.2
(-2.5)
P 		83.6
(-2.8)
P 		78.9
(-3)
P 		168
B 188.5
(3)
P 		102.7
(-1.8)
P 		80.9
(-3)
P 		144.9
(0.6)
P 		108
C 211.2
(3)
P 		105.7
(-3)
P 		126.4
(-1.8)
P 		112.9
(-2.6)
P 		106
avr 215.5
(3) 		100.5
(-2.8) 		97
(-3) 		112.2
(-2.2) 		119

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 146
1 2 3 4 5 6 range
A 101.6
(-2.5)
P 		208.5
(3)
P 		103
(-2.4)
P 		95.5
(-2.8)
P 		98
(-2.7)
P 		92.1
(-3)
P 		116
B 238.3
(3)
P 		200.7
(1.3)
P 		116
(-2.6)
P 		107.8
(-3)
P 		126.7
(-2.1)
P 		152
(-1)
P 		131
C 199.2
(3)
P 		178.5
(1.8)
P 		116.1
(-1.8)
P 		95.4
(-3)
P 		125.9
(-1.2)
P 		131.7
(-0.9)
P 		104
avr 179.7
(2) 		195.9
(3) 		111.7
(-2.2) 		99.6
(-3) 		116.9
(-1.9) 		125.3
(-1.4) 		96
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192278_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13364
Sequence Type Exemplar sequence
Representative Public ID T16A9.4 NCBI
Target Description T16A9.4 /REP_DB=WormBase Gene ID /WP=CE18259 /TR=SW:Q22523 /GB=CAB00879.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=endothelin converting enzyme 1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074119(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074119 NCBI Caenorhabditis elegans T16A9.4 (T16A9.4) mRNA, complete cds. 11/11 None
SNAP00000032390 ENSEMBL cdna:SNAP chromosome:CEL140:V:14218269:14222750:-1 11/11 None
GENEFINDER00000032393 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14218269:14222724:-1 11/11 None
T16A9.4 ENSEMBL cdna:known chromosome:CEL140:V:14217965:14223290:-1 gene:T16A9.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14218268-14223054(-) 100.0 100.0
Public Domain and Genome References
Gene Title endothelin converting enzyme 1
Ensembl T16A9.4
Entrez Gene 179925 Entrez gene
SwissProt Q22523 EMBL-EBI
WormBase CE18259 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4245 neprilysin activity inferred from electronic annotation QuickGO AmiGO
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB00879 Hypothetical protein T16A9.4 [Caenorhabditis elegans] ref|NP_506520.1| T16A9.4 [Caenorhabditis elegans] sp|Q22523|YCYL_CAEEL Hypothetical zinc metalloproteinase T16A9.4 0.0
blast CAE61001 Hypothetical protein CBG04739 [Caenorhabditis briggsae] 0.0
blast BAB33300.1 neutral endopeptidase 24.11 [Bombyx mori] 1.0E-128
Protein Domains
Database ID Description E-Value
Pfam IPR008753 EMBL-EBI Peptidase M13 5.5E-45
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 1.5E-70
Pfam IPR008753 EMBL-EBI Peptidase M13 1.3E-32
Pfam IPR008753 EMBL-EBI Peptidase M13 2.7E-8
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 1.5E-70
Pfam IPR008753 EMBL-EBI Peptidase M13 1.3E-32
Pfam IPR008753 EMBL-EBI Peptidase M13 2.7E-8
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 1.5E-70
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506520.1 1 27-49
T16A9.4 1 27-49
SNAP00000032390 1 21-43
GENEFINDER00000032393 1 13-35
Sequence
Target Sequence 
>C. ELEGANS:192278_S_AT
cccgcgggcatcatgcaattcccgtttctaactcttggagttccaaactacatcacttac
ggaatggttggagcagtcattggacatgaagtatctcatgcatttgatgatcagggagga
caatacgatgaaatggggaatttgaatgattggtgggatgctgaaacagaggaaaagttc
atcgagaaaacaagatgctttgtaaggcaatatgagaatgtgcacgtagttgaggcagat
attcatttgaacggtcaactaagtcttggagagaatattgctgataatggaggagtcaag
acagcttttaacgcgtacaaggcatggaaatcaaatacaacaggaatcagtgaacctgct
ctaccaggattccagaacttcaccagtcaacagatgttcttcttggcttatgcaaataac
tggtgttcattggttcgtccaaaacattacattcaaataattctaacagacgtgcatgct
ccatcaaaatatcgtgcaatgattccacttcaaaaccgtccagaattcgcgaaagcgttc
caatgtcctattg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCCGCGGGCATCATGCAATTCCCGT 303 257 1729 Antisense
CAATTCCCGTTTCTAACTCTTGGAG 345 177 1744 Antisense
GGAGTTCCAAACTACATCACTTACG 66 519 1765 Antisense
GTGAACCTGCTCTACCAGGATTCCA 283 481 2078 Antisense
GGATTCCAGAACTTCACCAGTCAAC 304 509 2095 Antisense
AGATGTTCTTCTTGGCTTATGCAAA 605 67 2120 Antisense
AACTGGTGTTCATTGGTTCGTCCAA 310 143 2146 Antisense
ATTCTAACAGACGTGCATGCTCCAT 409 11 2188 Antisense
GTGCAATGATTCCACTTCAAAACCG 238 481 2222 Antisense
AAACCGTCCAGAATTCGCGAAAGCG 418 123 2241 Antisense
GCGAAAGCGTTCCAATGTCCTATTG 82 289 2257 Antisense
Sequence Source Affymetrix Proprietary Database