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[192277_at] alpha-N-acetylgalactosaminidase

Description R07B7.11 /REP_DB=WormBase Gene ID /WP=CE06273 /TR=Q21801 /GB=CAB00120.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=alpha-N-acetylgalactosaminidase
Cluster 6grp_highest_10_10 > 35      6grp_high1_10_10 > 52      4grp_highest_8_8 > 17      4grp_high_8_8 > 61      6grp_highest_nonorm > 42      6grp_total > 30      4grp_total > 59      6grp_highest_sub > 03      6grp_cgc > 82      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1061
1 2 3 4 range
A 762.2
(0.3)
P 		994.2
(1.7)
P 		1231.9
(3)
P 		174.5
(-3)
P 		1057
B 873.6
(0.9)
P 		1235.4
(3)
P 		1194.6
(2.8)
P 		210.4
(-3)
P 		1025
C 781.3
(0.5)
P 		1077.8
(2.3)
P 		1184.7
(3)
P 		223.5
(-3)
P 		961
avr 805.7
(0.6) 		1102.5
(2.4) 		1203.7
(3) 		202.8
(-3) 		1001

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1071
1 2 3 4 5 6 range
A 599.7
(-2.4)
P 		918.8
(-0.6)
P 		1565.6
(3)
P 		1114.5
(0.5)
P 		558.1
(-2.6)
P 		494.7
(-3)
P 		1071
B 645.3
(-2.2)
P 		1137.4
(2.8)
P 		1156.7
(3)
P 		965.4
(1.1)
P 		633.8
(-2.3)
P 		564.8
(-3)
P 		592
C 585.4
(-2.3)
P 		1225
(2.6)
P 		1275.4
(3)
P 		912.3
(0.2)
P 		498.1
(-3)
P 		561.7
(-2.5)
P 		777
avr 610.1
(-2.5) 		1093.7
(1.2) 		1332.6
(3) 		997.4
(0.5) 		563.3
(-2.8) 		540.4
(-3) 		792
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 387
1 2 3 4 range
A 277.5
(2.1)
P 		215.8
(0.8)
P 		322.1
(3)
P 		25.5
(-3)
P 		297
B 278.6
(2.4)
P 		305.1
(3)
P 		276.8
(2.3)
P 		44.8
(-3)
P 		260
C 258.6
(0.5)
P 		309.9
(1.4)
P 		412.9
(3)
P 		37.7
(-3)
P 		375
avr 271.6
(1.7) 		276.9
(1.8) 		337.3
(3) 		36
(-3) 		301

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 268
1 2 3 4 5 6 range
A 110.6
(-3)
P 		326.8
(2.6)
P 		340.9
(3)
P 		282.7
(1.5)
P 		168.4
(-1.5)
P 		120.4
(-2.7)
P 		230
B 224.5
(-2.5)
P 		378.6
(3)
P 		280.9
(-0.5)
P 		255.7
(-1.4)
P 		257.5
(-1.3)
P 		210.6
(-3)
P 		168
C 217
(-1.4)
P 		373.8
(3)
P 		324.6
(1.6)
P 		183.5
(-2.4)
P 		161.2
(-3)
P 		188
(-2.2)
P 		213
avr 184
(-2.6) 		359.7
(3) 		315.5
(1.6) 		240.6
(-0.8) 		195.7
(-2.3) 		173
(-3) 		187
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192277_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12886
Sequence Type Exemplar sequence
Representative Public ID R07B7.11 NCBI
Target Description R07B7.11 /REP_DB=WormBase Gene ID /WP=CE06273 /TR=Q21801 /GB=CAB00120.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=alpha-N-acetylgalactosaminidase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073630(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073630 NCBI Caenorhabditis elegans R07B7.11 (alpha-N-acetylgalactosaminidase) mRNA, complete cds. 11/11 None
SNAP00000027092 ENSEMBL cdna:SNAP chromosome:CEL140:V:12089826:12091415:1 11/11 None
GENEFINDER00000027100 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:12089826:12091415:1 11/11 None
R07B7.11 ENSEMBL cdna:known chromosome:CEL140:V:12089822:12091497:1 gene:R07B7.11 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:12089825-12091415(+) 100.0 100.0
Public Domain and Genome References
Gene Title alpha-N-acetylgalactosaminidase
Gene Symbol gana-1
Ensembl R07B7.11
Entrez Gene 179660 Entrez gene
SwissProt Q21801 EMBL-EBI
WormBase CE06273 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:251729_AT alpha-galactosidase, putative / melibiase, putative / alpha-D-galactoside galactohydrolase, putative at
CANINE:1591567_AT similar to Alpha-N-acetylgalactosaminidase precursor (Alpha-galactosidase B) cfa
CANINE_2:CFA.19162.1.S1_S_AT similar to Alpha-N-acetylgalactosaminidase precursor (Alpha-galactosidase B) cfa
CANINE_2:CFAAFFX.2380.1.S1_AT similar to Alpha-N-acetylgalactosaminidase precursor (Alpha-galactosidase B) cfa
DROSOPHILA_2:1635163_AT dm
DROSGENOME1:146129_AT dm
CHICKEN:GGA.4369.1.S1_AT N-acetylgalactosaminidase, alpha- gga
HG-U133_PLUS_2:202943_S_AT N-acetylgalactosaminidase, alpha- hs
HG-U133A:202943_S_AT N-acetylgalactosaminidase, alpha- hs
HG-FOCUS:202943_S_AT N-acetylgalactosaminidase, alpha- hs
HG-U133A_2:202943_S_AT N-acetylgalactosaminidase, alpha- hs
HG-U133A_2:202944_AT N-acetylgalactosaminidase, alpha- hs
HG-U133A:202944_AT N-acetylgalactosaminidase, alpha- hs
HG-U133_PLUS_2:202944_AT N-acetylgalactosaminidase, alpha- hs
HU35KSUBA:M29276_S_AT N-acetylgalactosaminidase, alpha- hs
HUGENEFL:M62783_AT N-acetylgalactosaminidase, alpha- hs
U133_X3P:G4557780_3P_AT N-acetylgalactosaminidase, alpha- hs
U133_X3P:HS2.75372.2.A1_3P_S_AT N-acetylgalactosaminidase, alpha- hs
HG-U133_PLUS_2:1555041_A_AT N-acetylgalactosaminidase, alpha- hs
HG-U95AV2:36607_AT N-acetylgalactosaminidase, alpha- hs
MOE430A:1449043_AT N-acetyl galactosaminidase, alpha mm
MOUSE430A_2:1449043_AT N-acetyl galactosaminidase, alpha mm
MOUSE430_2:1449043_AT N-acetyl galactosaminidase, alpha mm
MG-U74AV2:103637_AT N-acetyl galactosaminidase, alpha mm
MU19KSUBB:TC33863_AT N-acetyl galactosaminidase, alpha, mRNA (cDNA clone MGC:13811 IMAGE:4019197) mm
MU19KSUBC:TC37202_AT N-acetyl galactosaminidase, alpha, mRNA (cDNA clone MGC:13811 IMAGE:4019197) mm
RG-U34A:RC_AA892847_AT N-acetyl galactosaminidase, alpha (predicted) rn
RAE230A:1382285_AT N-acetyl galactosaminidase, alpha (predicted) rn
RAT230_2:1382285_AT N-acetyl galactosaminidase, alpha (predicted) rn
RG-U34C:RC_AI235262_AT N-acetyl galactosaminidase, alpha (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
5975 carbohydrate metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4553 hydrolase activity, hydrolyzing O-glycosyl compounds inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB00120 Hypothetical protein R07B7.11 [Caenorhabditis elegans] ref|NP_506031.1| R07B7.11 [Caenorhabditis elegans] 0.0
blast CAE75386 Hypothetical protein CBG23373 [Caenorhabditis briggsae] 0.0
blast EAA14548 ENSANGP00000020847 [Anopheles gambiae str. PEST] ref|XP_318652.2| ENSANGP00000020847 [Anopheles gambiae str. PEST] 1.0E-108
Protein Domains
Database ID Description E-Value
Pfam IPR000111 EMBL-EBI Glycoside hydrolase, clan GH-D 3.2E-8
Sequence
Target Sequence 
>C. ELEGANS:192277_AT
ggatatgtctatttctcaattcactgtttggtgtatttggtcagctcctcttattatgtc
aaatgatttacgaattatcggtgatagtttcaaggatgttctcaaaaacaaggaagcaat
taaaattaatcaggatcctctcggaattatgggaagattgatcaaaaatagcaccgatat
cggagtttatgtaaaacaaattactccatcaaagggagacaagaaatcgtttgcatttgc
ttatctcaacaggaacgagaaagaaggatataaacgtattgaaattcaacttgcctcaat
tggtctcactgatcctgctggttactatgttcatgatatctggtcccatgttgatctcgg
tcttcttcgttcaggagacagtatagttgtatcaattgctccagctggaagtgtcttttt
ccgtgccgaccttgcatcgcttcatcaaccaaaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATATGTCTATTTCTCAATTCACT 278 511 795 Antisense
GTTTGGTGTATTTGGTCAGCTCCTC 16 453 820 Antisense
GTCAGCTCCTCTTATTATGTCAAAT 670 465 834 Antisense
TAATCAGGATCCTCTCGGAATTATG 597 635 921 Antisense
GTTTGCATTTGCTTATCTCAACAGG 380 451 1023 Antisense
TCAACTTGCCTCAATTGGTCTCACT 128 631 1080 Antisense
GATCCTGCTGGTTACTATGTTCATG 19 417 1105 Antisense
GATATCTGGTCCCATGTTGATCTCG 613 425 1129 Antisense
TCTCGGTCTTCTTCGTTCAGGAGAC 329 611 1149 Antisense
TAGTTGTATCAATTGCTCCAGCTGG 377 653 1178 Antisense
CTTGCATCGCTTCATCAACCAAAAG 212 217 1225 Antisense
Sequence Source Affymetrix Proprietary Database