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[192262_at]

Description R11A5.4 /REP_DB=WormBase Gene ID /WP=CE12728 /TR=O02286 /GB=CAB05600.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=phosphoenolpyruvate carboxykinase
Cluster 6grp_highest_10_10 > 47      6grp_high1_10_10 > 63      4grp_highest_8_8 > 54      4grp_high_8_8 > 09      6grp_highest_nonorm > 08      6grp_total > 63      4grp_total > 22      6grp_high1_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3409
1 2 3 4 range
A 14546.3
(2.7)
P 		14400.2
(2.1)
P 		13086.6
(-3)
P 		14618.6
(3)
P 		1532
B 14223.9
(2.2)
P 		11694.3
(-2.7)
P 		11561.4
(-3)
P 		14607.2
(3)
P 		3046
C 14146.4
(3)
P 		11209.7
(-3)
P 		11667.6
(-2.1)
P 		12671.7
(-0)
P 		2937
avr 14305.5
(3) 		12434.7
(-2.1) 		12105.2
(-3) 		13965.8
(2.1) 		2200

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9712
1 2 3 4 5 6 range
A 15800.3
(-3)
P 		15778.7
(-3)
P 		23908.5
(3)
P 		19066.1
(-0.6)
P 		16755.4
(-2.3)
P 		20239.4
(0.3)
P 		8130
B 15243.9
(-3)
P 		16106.5
(-2.4)
P 		24308.5
(3)
P 		20139.8
(0.3)
P 		15206.3
(-3)
P 		17917.2
(-1.2)
P 		9102
C 14596.7
(-3)
P 		16810.7
(-1.3)
P 		22286.4
(3)
P 		20568
(1.7)
P 		17642.6
(-0.6)
P 		18643.6
(0.2)
P 		7690
avr 15213.6
(-3) 		16232
(-2.3) 		23501.1
(3) 		19924.6
(0.4) 		16534.8
(-2) 		18933.4
(-0.3) 		8288
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3160
1 2 3 4 range
A 5296.3
(3)
P 		3125.9
(-1.1)
P 		3421.1
(-0.6)
P 		2139.9
(-3)
P 		3156
B 4535.9
(3)
P 		2888.2
(-2.3)
P 		2679
(-3)
P 		3112.8
(-1.6)
P 		1857
C 4682.9
(3)
P 		3222.9
(-0.4)
P 		4066.5
(1.5)
P 		2136.6
(-3)
P 		2546
avr 4838.4
(3) 		3079
(-1.4) 		3388.9
(-0.7) 		2463.1
(-3) 		2375

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3769
1 2 3 4 5 6 range
A 2912.7
(-3)
P 		5611.7
(3)
P 		5206.8
(2.1)
P 		4835.6
(1.3)
P 		5056.1
(1.8)
P 		4927.7
(1.5)
P 		2699
B 5304.1
(-3)
P 		5361.2
(-2.8)
P 		5902.8
(-0.4)
P 		5334.3
(-2.9)
P 		6178.1
(0.8)
P 		6681.2
(3)
P 		1377
C 5410.4
(0.6)
P 		5129.5
(-0.2)
P 		5672.3
(1.4)
P 		4136.2
(-3)
P 		5708.9
(1.5)
P 		6241.1
(3)
P 		2105
avr 4542.4
(-3) 		5367.5
(0.5) 		5594
(1.5) 		4768.7
(-2) 		5647.7
(1.7) 		5950
(3) 		1408
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192262_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2299
Sequence Type Exemplar sequence
Representative Public ID R11A5.4 NCBI
Target Description R11A5.4 /REP_DB=WormBase Gene ID /WP=CE12728 /TR=O02286 /GB=CAB05600.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=phosphoenolpyruvate carboxykinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026418(11), NM_001026419(11), NM_001026416(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026418 NCBI Caenorhabditis elegans R11A5.4c (R11A5.4) mRNA, complete cds. 11/11 None
NM_001026419 NCBI Caenorhabditis elegans R11A5.4d (R11A5.4) mRNA, complete cds. 11/11 None
NM_001026416 NCBI Caenorhabditis elegans R11A5.4a (R11A5.4) mRNA, complete cds. 11/11 None
SNAP00000037586 ENSEMBL cdna:SNAP chromosome:CEL140:I:7876065:7880009:1 11/11 None
GENEFINDER00000037592 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:7877627:7880009:1 11/11 None
R11A5.4c.3 ENSEMBL cdna:known chromosome:CEL140:I:7874309:7880009:1 gene:R11A5.4 11/11 None
R11A5.4d.1 ENSEMBL cdna:known chromosome:CEL140:I:7874473:7880009:1 gene:R11A5.4 11/11 None
R11A5.4d.5 ENSEMBL cdna:known chromosome:CEL140:I:7874482:7880009:1 gene:R11A5.4 11/11 None
R11A5.4d.3 ENSEMBL cdna:known chromosome:CEL140:I:7874482:7880009:1 gene:R11A5.4 11/11 None
R11A5.4d.2 ENSEMBL cdna:known chromosome:CEL140:I:7874482:7880009:1 gene:R11A5.4 11/11 None
R11A5.4c.2 ENSEMBL cdna:known chromosome:CEL140:I:7874482:7880009:1 gene:R11A5.4 11/11 None
R11A5.4a.2 ENSEMBL cdna:known chromosome:CEL140:I:7874527:7880248:1 gene:R11A5.4 11/11 None
R11A5.4a.1 ENSEMBL cdna:known chromosome:CEL140:I:7874527:7880206:1 gene:R11A5.4 11/11 None
R11A5.4d.4 ENSEMBL cdna:known chromosome:CEL140:I:7874608:7880009:1 gene:R11A5.4 11/11 None
R11A5.4c.1 ENSEMBL cdna:known chromosome:CEL140:I:7877890:7880009:1 gene:R11A5.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001026417 5/11 Cross Hyb Matching Probes None
R11A5.4b 5/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
172556 4 3 NM_001026416 192262_at (11)
NM_001026418 192262_at (11)
NM_001026419 192262_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:7874457-7879940(+) 100.0 100.0
Public Domain and Genome References
Ensembl R11A5.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05600 Hypothetical protein R11A5.4a [Caenorhabditis elegans] ref|NP_001021587.1| R11A5.4a [Caenorhabditis elegans] 0.0
blast CAF31483 Hypothetical protein R11A5.4d [Caenorhabditis elegans] ref|NP_001021590.1| R11A5.4d [Caenorhabditis elegans] 0.0
blast CAF31482 Hypothetical protein R11A5.4c [Caenorhabditis elegans] ref|NP_001021589.1| R11A5.4c [Caenorhabditis elegans] 0.0
blast CAE63747 Hypothetical protein CBG08279 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR008209 EMBL-EBI Phosphoenolpyruvate carboxykinase (GTP) 1.0E-126
Pfam IPR008209 EMBL-EBI Phosphoenolpyruvate carboxykinase (GTP) 1.0E-126
Pfam IPR008209 EMBL-EBI Phosphoenolpyruvate carboxykinase (GTP) 1.0E-126
Pfam IPR008209 EMBL-EBI Phosphoenolpyruvate carboxykinase (GTP) 1.0E-126
Pfam IPR008209 EMBL-EBI Phosphoenolpyruvate carboxykinase (GTP) 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:192262_AT
gaggctatcattttcggaggacgtcgtccacagggagtaccactcatttatgagactaac
tcatgggaacacggagtcttcactggatcatgcctcaagtcagaagctaccgctgcggcc
gaattcactggaaagactgttatgcacgatccaatggccatgcgtccattcatgggatac
aattttggaaagtatctccaacattggttggatctaaaaaccgactccagaaagatgcca
aagatctaccacgtcaattggttccgtaaggattcaaataacaagttcttgtggccagga
ttcggagacaacatccgtgttatcgactggatcatcagaagactcgatggagaacaagaa
atcggagtggaaaccccaatcggaacagttccagccaagggatccatcaacttggaagga
ctcggagaggtcaactgggatgagctcatgtctgtgccagccgactactggaaacaagat
gctcaagaaatccgcaagttcctcgatgaacaagttggagaagatcttccagaaccagtt
cgtgccga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGGCTATCATTTTCGGAGGACGTC 580 405 1393 Antisense
GGAACACGGAGTCTTCACTGGATCA 667 527 1458 Antisense
ATGCCTCAAGTCAGAAGCTACCGCT 522 41 1482 Antisense
ATCCAATGGCCATGCGTCCATTCAT 663 39 1541 Antisense
ATGCCAAAGATCTACCACGTCAATT 303 41 1627 Antisense
GAAACCCCAATCGGAACAGTTCCAG 392 357 1762 Antisense
GTTCCAGCCAAGGGATCCATCAACT 433 441 1780 Antisense
TGGGATGAGCTCATGTCTGTGCCAG 156 555 1828 Antisense
GTCTGTGCCAGCCGACTACTGGAAA 126 471 1842 Antisense
AGAAATCCGCAAGTTCCTCGATGAA 700 71 1878 Antisense
ATCTTCCAGAACCAGTTCGTGCCGA 201 31 1916 Antisense
Sequence Source Affymetrix Proprietary Database