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[192250_at] PH (pleckstrin homology) domain

Description F52D10.6 /REP_DB=WormBase Gene ID /WP=CE03392 /TR=Q20653 /GB=CAA91472.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=PH (pleckstrin homology) domain
Cluster 4grp_high_8_8 > 17      6grp_total > 14      4grp_total > 38      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 119
1 2 3 4 range
A 183.9
(2.8)
P 		118.2
(-1)
P 		82.5
(-3)
A 		188.2
(3)
P 		106
B 177.6
(3)
P 		126.4
(0.1)
P 		99.5
(-1.4)
P 		71.2
(-3)
A 		106
C 186.4
(3)
P 		129.8
(0.1)
A 		68.9
(-3)
A 		98.5
(-1.5)
P 		118
avr 182.6
(3) 		124.8
(-0.5) 		83.6
(-3) 		119.3
(-0.8) 		99

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 174
1 2 3 4 5 6 range
A 181.3
(3)
A 		161.8
(2.1)
P 		98.4
(-0.8)
P 		51.3
(-3)
A 		67
(-2.3)
A 		53.1
(-2.9)
A 		130
B 177.4
(3)
P 		153.1
(2.1)
P 		103.8
(0.4)
P 		108.2
(0.6)
P 		11.9
(-2.8)
A 		7.2
(-3)
A 		170
C 127.1
(1.7)
P 		158.9
(3)
P 		98.6
(0.6)
M 		90.1
(0.2)
A 		62.2
(-0.9)
A 		10.5
(-3)
A 		148
avr 161.9
(3) 		157.9
(2.8) 		100.3
(0.3) 		83.2
(-0.4) 		47
(-2) 		23.6
(-3) 		138
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 52
1 2 3 4 range
A 66.9
(3)
P 		25.7
(-2.5)
P 		21.6
(-3)
A 		27.6
(-2.2)
P 		45
B 56.6
(3)
P 		31.2
(-0.7)
P 		23.1
(-1.9)
P 		15.2
(-3)
A 		41
C 61.7
(3)
P 		37.3
(-0.2)
A 		24
(-2)
A 		16.6
(-3)
P 		45
avr 61.7
(3) 		31.4
(-1.3) 		22.9
(-2.6) 		19.8
(-3) 		42

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 59
1 2 3 4 5 6 range
A 33.4
(-0.2)
A 		57.6
(3)
P 		21.4
(-1.9)
P 		13
(-3)
A 		20.2
(-2)
A 		12.9
(-3)
A 		45
B 61.7
(3)
P 		51
(1.9)
P 		25.2
(-0.7)
P 		28.7
(-0.4)
P 		4.8
(-2.8)
A 		2.7
(-3)
A 		59
C 47.1
(2.8)
P 		48.5
(3)
P 		25.1
(-0.1)
M 		18.1
(-1.1)
A 		20.1
(-0.8)
A 		3.5
(-3)
A 		45
avr 47.4
(2.3) 		52.4
(3) 		23.9
(-0.7) 		19.9
(-1.2) 		15
(-1.9) 		6.4
(-3) 		46
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192250_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16196
Sequence Type Exemplar sequence
Representative Public ID F52D10.6 NCBI
Target Description F52D10.6 /REP_DB=WormBase Gene ID /WP=CE03392 /TR=Q20653 /GB=CAA91472.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=PH (pleckstrin homology) domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077534(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077534 NCBI Caenorhabditis elegans F52D10.6 (F52D10.6) mRNA, complete cds. 11/11 None
SNAP00000034605 ENSEMBL cdna:SNAP chromosome:CEL140:X:11583004:11584880:1 11/11 None
GENEFINDER00000034612 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:11583026:11584880:1 11/11 None
F52D10.6 ENSEMBL cdna:known chromosome:CEL140:X:11583026:11584880:1 gene:F52D10.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:11583025-11584880(+) 100.0 100.0
Public Domain and Genome References
Gene Title PH (pleckstrin homology) domain
Ensembl F52D10.6
Entrez Gene 186110 Entrez gene
SwissProt Q20653 EMBL-EBI
WormBase CE03392 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA91472 Hypothetical protein F52D10.6 [Caenorhabditis elegans] ref|NP_509935.1| F52D10.6 [Caenorhabditis elegans] 0.0
blast CAE70612 Hypothetical protein CBG17295 [Caenorhabditis briggsae] 0.0
blast XP_534170 PREDICTED: similar to FERM, RhoGEF, and pleckstrin domain protein 1 isoform 1 [Canis familiaris] 8.0E-43
Protein Domains
Database ID Description E-Value
Pfam IPR001849 EMBL-EBI Pleckstrin-like 9.3E-21
Pfam IPR001849 EMBL-EBI Pleckstrin-like 9.3E-21
Sequence
Target Sequence 
>C. ELEGANS:192250_AT
taatcactctccacaacgcagatatctctattgtttttgctgcaccagatcgtgcatctt
ggattgaagacattacagaagctattaaaaatgcggctagagcaaagatagatctaccta
gtttggtaatggagaagaaagaggaaaatgatattgatatgacaaaagtgttgctaccag
aagaatctcccgtgtcaaaaatgagcccattgcaaatttgctggtatcgaaaatgctcgt
ttggaagaaaagatgtcaataaaatgataacaaataccatgtgtggatatttgaagcgga
aactgcgcaattcatccgggtggcaggatttatgggtggtcatgtgttgtcacacactgt
acttttatagaaatcataacgagagagaaccacttgcacatttgtccctcatggattatg
gtgtaggactgccaacggttgctgacaagatcgacaaccatgagaactgcttcaagttgt
tctacggttcacacacctactttttccgcaccgattcctattacttttttga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAATCACTCTCCACAACGCAGATAT 525 635 644 Antisense
ATATCTCTATTGTTTTTGCTGCACC 269 21 665 Antisense
GCACCAGATCGTGCATCTTGGATTG 434 315 685 Antisense
TGCTACCAGAAGAATCTCCCGTGTC 115 581 815 Antisense
TGCGCAATTCATCCGGGTGGCAGGA 169 585 947 Antisense
GAGAGAACCACTTGCACATTTGTCC 386 393 1026 Antisense
GCACATTTGTCCCTCATGGATTATG 67 317 1039 Antisense
GTAGGACTGCCAACGGTTGCTGACA 160 455 1066 Antisense
GATCGACAACCATGAGAACTGCTTC 648 417 1092 Antisense
AGTTGTTCTACGGTTCACACACCTA 387 65 1118 Antisense
GCACCGATTCCTATTACTTTTTTGA 438 273 1151 Antisense
Sequence Source Affymetrix Proprietary Database