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[192249_at] DNA binding protein, ETS domain

Description F22A3.1 /REP_DB=WormBase Gene ID /WP=CE04437 /TR=Q19695 /GB=AAA83194.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=DNA binding protein, ETS domain
Cluster 6grp_total > 02      4grp_total > 52      6grp_cgc > 41      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 380
1 2 3 4 range
A 342.2
(2.6)
M 		364.2
(3)
P 		205.4
(-0)
A 		46.7
(-3)
A 		318
B 269.4
(1.5)
P 		354
(3)
P 		188.9
(0.1)
A 		11.8
(-3)
A 		342
C 295.2
(1.4)
P 		392.2
(3)
M 		243.3
(0.6)
P 		25.6
(-3)
A 		367
avr 302.3
(1.8) 		370.1
(3) 		212.5
(0.2) 		28
(-3) 		342

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 241
1 2 3 4 5 6 range
A 328.4
(3)
P 		303.6
(2.3)
P 		169
(-1.4)
M 		164.1
(-1.5)
A 		184.8
(-1)
A 		111
(-3)
A 		217
B 268.9
(0.6)
M 		351.7
(3)
P 		212.4
(-1)
A 		246.9
(-0)
P 		207.6
(-1.1)
A 		142.9
(-3)
A 		209
C 291.5
(2.1)
P 		322.6
(3)
P 		217.6
(-0.1)
A 		214.7
(-0.2)
M 		219.9
(-0.1)
P 		120.7
(-3)
A 		202
avr 296.3
(2.1) 		326
(3) 		199.7
(-0.8) 		208.6
(-0.5) 		204.1
(-0.6) 		124.9
(-3) 		201
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 122
1 2 3 4 range
A 124.6
(3)
M 		79.1
(0.7)
P 		53.7
(-0.6)
A 		6.8
(-3)
A 		118
B 85.9
(2.9)
P 		87.4
(3)
P 		43.8
(-0.1)
A 		2.5
(-3)
A 		85
C 97.7
(2.2)
P 		112.7
(3)
M 		84.8
(1.5)
P 		4.3
(-3)
A 		108
avr 102.7
(3) 		93.1
(2.4) 		60.8
(0.4) 		4.5
(-3) 		98

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 90
1 2 3 4 5 6 range
A 60.5
(-0.5)
P 		108
(3)
P 		36.8
(-2.3)
M 		41.6
(-1.9)
A 		55.8
(-0.9)
A 		27
(-3)
A 		81
B 93.6
(0.8)
M 		117.1
(3)
P 		51.6
(-3)
A 		65.4
(-1.7)
P 		84.3
(-0)
A 		53.3
(-2.8)
A 		66
C 108.1
(3)
P 		98.4
(2.1)
P 		55.4
(-1.7)
A 		43.2
(-2.8)
M 		71.1
(-0.3)
P 		40.4
(-3)
A 		68
avr 87.4
(1.2) 		107.8
(3) 		47.9
(-2.3) 		50.1
(-2.1) 		70.4
(-0.3) 		40.2
(-3) 		68
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192249_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16340
Sequence Type Exemplar sequence
Representative Public ID F22A3.1 NCBI
Target Description F22A3.1 /REP_DB=WormBase Gene ID /WP=CE04437 /TR=Q19695 /GB=AAA83194.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=DNA binding protein, ETS domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076698(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076698 NCBI Caenorhabditis elegans F22A3.1 (F22A3.1) mRNA, complete cds. 11/11 None
GENEFINDER00000004720 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:6516338:6522017:1 11/11 None
SNAP00000004712 ENSEMBL cdna:SNAP chromosome:CEL140:X:6521141:6521869:1 10/11 None
F22A3.1 ENSEMBL cdna:known chromosome:CEL140:X:6519289:6522604:1 gene:F22A3.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:6516337-6521869(+) 100.0 100.0
Public Domain and Genome References
Gene Title DNA binding protein, ETS domain
Gene Symbol ets-4
Ensembl F22A3.1
Entrez Gene 180927 Entrez gene
SwissProt Q19695 EMBL-EBI
WormBase CE34001 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
3700 transcription factor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_509099 F22A3.1 [Caenorhabditis elegans] gb|AAA83194.3| Hypothetical protein F22A3.1 [Caenorhabditis elegans] 0.0
blast CAE65748 Hypothetical protein CBG10834 [Caenorhabditis briggsae] 0.0
blast NP_509099 F22A3.1 [Caenorhabditis elegans] gb|AAA83194.3| Hypothetical protein F22A3.1 [Caenorhabditis elegans] 4.0E-90
blast CAE65748 Hypothetical protein CBG10834 [Caenorhabditis briggsae] 3.0E-82
blast XP_396511 PREDICTED: similar to CG5583-PA [Apis mellifera] 4.0E-38
Protein Domains
Database ID Description E-Value
Pfam IPR003118 EMBL-EBI Sterile alpha motif (SAM)/Pointed domain 8.5E-36
Pfam IPR000418 EMBL-EBI Ets-domain 6.7E-42
Pfam IPR003118 EMBL-EBI Sterile alpha motif (SAM)/Pointed domain 4.0E-34
Pfam IPR000418 EMBL-EBI Ets-domain 7.3E-33
Pfam IPR000418 EMBL-EBI Ets-domain 6.7E-42
Sequence
Target Sequence 
>C. ELEGANS:192249_AT
ctagccagcaccaacaatcaaacaaacaacatggcagctgctgagaacccgaatcatcca
tttttcaatggaaacggagggtatccaaacatgtcgatgagcagcttctttcaacaggga
actgttttgccatcgccaagcaacagcgataccagcagcaatgggagcagtcaagacatg
aatgacgatgatattgatcttcacatgaacaattcgaactgtggtttctcaaacttcttt
cacaaccagggttacatgaactcccctattgacgcaatgtgcaacggaagcgagggagat
gacgatgagcgtgcctacactcgtcatcaaggaactgttcatctgtggcagtttattcgt
gagcttttggatcaaccaaaacagtacagtgcatgtgttcgatgggtggatagagatgag
ggaacttttaaaatcgagtcttccctcctgctggctagatattggg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTAGCCAGCACCAACAATCAAACAA 461 225 1006 Antisense
ATGGCAGCTGCTGAGAACCCGAATC 146 49 1036 Antisense
GAGCAGCTTCTTTCAACAGGGAACT 484 389 1104 Antisense
ACTGTTTTGCCATCGCCAAGCAACA 318 97 1126 Antisense
TGGTTTCTCAAACTTCTTTCACAAC 699 551 1227 Antisense
GGGTTACATGAACTCCCCTATTGAC 218 497 1254 Antisense
GATGACGATGAGCGTGCCTACACTC 152 411 1303 Antisense
GTGCCTACACTCGTCATCAAGGAAC 43 479 1316 Antisense
GTGGCAGTTTATTCGTGAGCTTTTG 475 491 1350 Antisense
TTTTAAAATCGAGTCTTCCCTCCTG 19 673 1431 Antisense
TCCCTCCTGCTGGCTAGATATTGGG 522 595 1447 Antisense
Sequence Source Affymetrix Proprietary Database