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[192247_at]

Description C33D9.1 /REP_DB=WormBase Gene ID /WP=CE03042 /TR=Q18372 /GB=CAA92282.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=PH (pleckstrin homology) domain
Cluster 6grp_total > 26      4grp_total > 23      6grp_cgc > 23      6grp_cgc_sub > De      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 452
1 2 3 4 range
A 419.5
(3)
A 		41.1
(-3)
A 		53.9
(-2.8)
A 		326
(1.5)
P 		378
B 493.5
(3)
P 		86.2
(-2.8)
A 		73.6
(-3)
A 		371.1
(1.3)
A 		420
C 433.4
(3)
A 		41.8
(-3)
A 		142
(-1.5)
A 		203.2
(-0.5)
A 		392
avr 448.8
(3) 		56.4
(-3) 		89.8
(-2.5) 		300.1
(0.7) 		392

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 276
1 2 3 4 5 6 range
A 366.7
(3)
M 		324.2
(1.9)
A 		134.3
(-3)
A 		266.2
(0.4)
A 		168.7
(-2.1)
P 		292.7
(1.1)
P 		232
B 369.6
(3)
M 		286.2
(0.5)
A 		277.7
(0.2)
A 		196
(-2.2)
P 		323.4
(1.6)
M 		170.9
(-3)
P 		199
C 286.6
(1.7)
A 		342.2
(3)
A 		250.6
(0.8)
A 		268
(1.2)
A 		94.1
(-3)
A 		203.7
(-0.3)
P 		248
avr 341
(3) 		317.5
(2) 		220.9
(-1.9) 		243.4
(-1) 		195.4
(-3) 		222.4
(-1.9) 		146
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 149
1 2 3 4 range
A 152.7
(3)
A 		8.9
(-3)
A 		14.1
(-2.8)
A 		47.7
(-1.4)
P 		144
B 157.4
(3)
P 		21.3
(-2.8)
A 		17.1
(-3)
A 		79.1
(-0.3)
A 		140
C 143.5
(3)
A 		12
(-3)
A 		49.5
(-1.3)
A 		34.3
(-2)
A 		132
avr 151.2
(3) 		14.1
(-3) 		26.9
(-2.4) 		53.7
(-1.3) 		137

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 102
1 2 3 4 5 6 range
A 67.6
(-0.3)
M 		115.3
(3)
A 		29.2
(-3)
A 		67.5
(-0.3)
A 		50.9
(-1.5)
P 		71.3
(-0.1)
P 		86
B 128.6
(2.8)
M 		95.3
(0.3)
A 		67.4
(-1.8)
A 		51.9
(-3)
P 		131.4
(3)
M 		63.7
(-2.1)
P 		80
C 106.2
(3)
A 		104.4
(2.9)
A 		63.8
(-0.4)
A 		53.9
(-1.1)
A 		30.4
(-3)
A 		68.2
(-0)
P 		76
avr 100.8
(2.5) 		105
(3) 		53.5
(-3) 		57.8
(-2.5) 		70.9
(-1) 		67.7
(-1.3) 		52
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192247_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9863
Sequence Type Exemplar sequence
Representative Public ID C33D9.1 NCBI
Target Description C33D9.1 /REP_DB=WormBase Gene ID /WP=CE03042 /TR=Q18372 /GB=CAA92282.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=PH (pleckstrin homology) domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027867(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027867 NCBI Caenorhabditis elegans EXCretory canal abnormal family member (exc-5) (exc-5) mRNA, complete cds. 11/11 None
GENEFINDER00000033381 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8799451:8801914:-1 11/11 None
C33D9.1a ENSEMBL cdna:known chromosome:CEL140:IV:8799074:8804839:-1 gene:C33D9.1 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001027868 7/11 Cross Hyb Matching Probes None
SNAP00000033377 7/11 Cross Hyb Matching Probes None
C33D9.1b 7/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177692 2 1 NM_001027867 192247_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8799456-8805664(-) 100.0 100.0
Public Domain and Genome References
Ensembl C33D9.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92282 Hypothetical protein C33D9.1a [Caenorhabditis elegans] ref|NP_001023038.1| EXCretory canal abnormal family member (exc-5) [Caenorhabditis elegans] gb|AAL50355.1| putative Rho guanine nucleotide exchange factor FGD-1 [Caenorhabditis elegans] 0.0
blast CAD30432 Hypothetical protein C33D9.1b [Caenorhabditis elegans] ref|NP_001023039.1| EXCretory canal abnormal family member (exc-5) [Caenorhabditis elegans] gb|AAL50361.1| Rho guanine nucleotide exchange factor FGD-1 [Caenorhabditis elegans] dbj|BAB43906.1| putative GDP-GTP exchange factor [Caenorhabditis elegans] 0.0
blast CAE56377 Hypothetical protein CBG24054 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001849 EMBL-EBI Pleckstrin-like 3.8E-20
Pfam IPR000219 EMBL-EBI DH domain 5.7E-38
Pfam IPR000219 EMBL-EBI DH domain 5.7E-38
Pfam IPR001849 EMBL-EBI Pleckstrin-like 3.8E-20
Sequence
Target Sequence 
>C. ELEGANS:192247_AT
tgaatttgggttcctatcacggggttcgaaatgtgtcaaatgcagtcgtcgtctctgtaa
aaaatgttttggtcgtcgcagaaatgagtcgaaaaagcacagaatatgtgatacatgcac
caaaaatatggatctagatggaattccaagaagcttttcaactcaaagtaatatgcgaag
gaatcttcttcaactaccagctaagggttcaggagtattgcattcaagccagattaggca
ccttcgccaaaaaccagagctccaacaaatctttcataactgctattatctattccgtgg
atctttggggaaattgttagatcgatttatggttgttcgagacgatttctgtatgtacac
ttattcatcagaagaggatatatgtgccttggctatgctgccacttccaggatgtgaagt
aaagatgtgtggagaaaaattcacattttcggttcgagtcggagcccgtcgaatgtacac
aatgaccgctgaagacgaacaaacccaaatgaaatggttggcgattttggatttagccgc
aaacgcacatctgaaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAATTTGGGTTCCTATCACGGGGT 413 327 2049 Antisense
GTCAAATGCAGTCGTCGTCTCTGTA 686 463 2083 Antisense
GAATCTTCTTCAACTACCAGCTAAG 398 327 2229 Antisense
CAAGCCAGATTAGGCACCTTCGCCA 88 187 2273 Antisense
GCACCTTCGCCAAAAACCAGAGCTC 87 315 2286 Antisense
ACCAGAGCTCCAACAAATCTTTCAT 51 87 2301 Antisense
TAACTGCTATTATCTATTCCGTGGA 368 639 2325 Antisense
TATGTGCCTTGGCTATGCTGCCACT 58 657 2429 Antisense
CATTTTCGGTTCGAGTCGGAGCCCG 587 213 2492 Antisense
CGGAGCCCGTCGAATGTACACAATG 330 243 2508 Antisense
TTAGCCGCAAACGCACATCTGAAGA 147 681 2581 Antisense
Sequence Source Affymetrix Proprietary Database