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[192235_at]

Description F20B6.8 /REP_DB=WormBase Gene ID /WP=CE04430 /TR=Q19632 /GB=AAA82316.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=serine-threonine protein kinase
Cluster 6grp_high1_10_10 > 37      4grp_high_8_8 > 03      6grp_total > 17      4grp_total > 14      6grp_cgc > 06      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 493
1 2 3 4 range
A 475.7
(1.7)
P 		222
(-2)
P 		152.4
(-3)
M 		560.8
(3)
M 		408
B 393.4
(-0.2)
P 		177.4
(-3)
P 		225.4
(-2.4)
P 		645.3
(3)
P 		468
C 442.4
(2.5)
P 		335.1
(-0.1)
P 		219.5
(-3)
P 		460.6
(3)
A 		241
avr 437.2
(1) 		244.8
(-2.2) 		199.1
(-3) 		555.6
(3) 		357

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 725
1 2 3 4 5 6 range
A 336.1
(0.9)
P 		331.2
(0.8)
P 		192.9
(-2.8)
P 		233.9
(-1.8)
P 		186.7
(-3)
P 		416.5
(3)
P 		230
B 302.3
(3)
P 		207.3
(0.2)
P 		238.3
(1.1)
P 		199.7
(0)
P 		96.8
(-3)
P 		256.9
(1.7)
P 		206
C 821.7
(3)
P 		244.8
(-2.3)
P 		305.9
(-1.7)
P 		174.3
(-3)
A 		233.2
(-2.4)
P 		170.1
(-3)
P 		652
avr 486.7
(3) 		261.1
(-1.3) 		245.7
(-1.6) 		202.6
(-2.4) 		172.2
(-3) 		281.2
(-0.9) 		315
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 133
1 2 3 4 range
A 173.2
(3)
P 		48.2
(-2.6)
P 		39.8
(-3)
M 		82.1
(-1.1)
M 		133
B 125.5
(2.2)
P 		43.8
(-3)
P 		52.2
(-2.5)
P 		137.5
(3)
P 		94
C 146.4
(3)
P 		96.3
(-1.3)
P 		76.5
(-3)
P 		77.7
(-2.9)
A 		70
avr 148.4
(3) 		62.8
(-2.6) 		56.2
(-3) 		99.1
(-0.2) 		92

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 270
1 2 3 4 5 6 range
A 62
(-1.4)
P 		117.8
(3)
P 		42
(-3)
P 		59.3
(-1.6)
P 		56.3
(-1.9)
P 		101.4
(1.7)
P 		76
B 105.2
(3)
P 		69
(-0.3)
P 		57.9
(-1.3)
P 		52.9
(-1.8)
P 		39.3
(-3)
P 		95.8
(2.1)
P 		66
C 304.6
(3)
P 		74.7
(-2.1)
P 		77.8
(-2)
P 		35
(-3)
A 		75.5
(-2.1)
P 		57
(-2.5)
P 		270
avr 157.3
(3) 		87.2
(-0.9) 		59.2
(-2.4) 		49.1
(-3) 		57
(-2.6) 		84.7
(-1) 		108
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192235_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16259
Sequence Type Exemplar sequence
Representative Public ID F20B6.8 NCBI
Target Description F20B6.8 /REP_DB=WormBase Gene ID /WP=CE04430 /TR=Q19632 /GB=AAA82316.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=serine-threonine protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171662(11), NM_171664(11), NM_171663(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171662 NCBI Caenorhabditis elegans Homeodomain interacting Protein Kinase family member (hpk-1) (hpk-1) mRNA, complete cds. 11/11 A 
NM_171664 NCBI Caenorhabditis elegans Homeodomain interacting Protein Kinase family member (hpk-1) (hpk-1) mRNA, complete cds. 11/11 None
NM_171663 NCBI Caenorhabditis elegans Homeodomain interacting Protein Kinase family member (hpk-1) (hpk-1) mRNA, complete cds. 11/11 None
SNAP00000010539 ENSEMBL cdna:SNAP chromosome:CEL140:X:4211684:4214590:-1 11/11 None
F20B6.8c.5 ENSEMBL cdna:known chromosome:CEL140:X:4210557:4216423:-1 gene:F20B6.8 11/11 None
F20B6.8c.2 ENSEMBL cdna:known chromosome:CEL140:X:4210557:4217264:-1 gene:F20B6.8 11/11 A 
F20B6.8c.3 ENSEMBL cdna:known chromosome:CEL140:X:4210557:4214535:-1 gene:F20B6.8 11/11 None
F20B6.8c.1 ENSEMBL cdna:known chromosome:CEL140:X:4210557:4214710:-1 gene:F20B6.8 11/11 None
F20B6.8b ENSEMBL cdna:known chromosome:CEL140:X:4210557:4217264:-1 gene:F20B6.8 11/11 A 
F20B6.8c.4 ENSEMBL cdna:known chromosome:CEL140:X:4210557:4214537:-1 gene:F20B6.8 11/11 None
F20B6.8a ENSEMBL cdna:known chromosome:CEL140:X:4211684:4214669:-1 gene:F20B6.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4211684-4214670(-) 100.0 100.0
Public Domain and Genome References
Ensembl F20B6.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM54186 Homeodomain interacting protein kinase protein 1, isoform b [Caenorhabditis elegans] ref|NP_741761.1| Homeodomain interacting Protein Kinase family member (hpk-1) [Caenorhabditis elegans] 0.0
blast AAA82316 Homeodomain interacting protein kinase protein 1, isoform a [Caenorhabditis elegans] ref|NP_741762.1| Homeodomain interacting Protein Kinase family member (hpk-1) [Caenorhabditis elegans] 0.0
blast AAM54187 Homeodomain interacting protein kinase protein 1, isoform c [Caenorhabditis elegans] ref|NP_741763.1| Homeodomain interacting Protein Kinase family member (hpk-1) [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 2.0E-17
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 1.0E-17
hanks 3.1.4 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; Cdk4 1.0E-56
hanks 3.1.4 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; Cdk4 7.0E-57
hanks 3.1.4 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; Cdk4 4.0E-57
hanks 3.1.4 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; Cdk4 3.0E-54
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 9.4E-61
Pfam IPR000719 EMBL-EBI Protein kinase 9.4E-61
Pfam IPR000719 EMBL-EBI Protein kinase 9.4E-61
Pfam IPR000719 EMBL-EBI Protein kinase 9.4E-61
Sequence
Target Sequence 
>C. ELEGANS:192235_AT
caatgcttcagggacagtggccaccaggggccgcgcaacagttcgccgttctcgcaaatg
acataatgcgagtaccggcaccacaaggaatcaatcagatgtttgcatcaacgccacaga
ctttctcacttccgcagtttctgtcgtcttccatcccaagtgcaactaccgcattcaacg
gaaacgcaccaaacattccatttcctgaagaaaattctagttgggcactcggcacagctg
cacaacaacaacagcaacaagctcaacgagcacaatcaatgataaacggaaatgtcaaag
tgaagccgctggcggcacaacctaagaagaactccccagctccgtcggtcatcacattat
ccagcgatgaagatagcaatggtgcaggatcaagtaatagtggaagtactacacgaactg
gcgccgtcaatccagtgagaaacgatacacttccaatgggaaataccatcaagaccgagg
atattcttgttccaccaaccacttttgatggacagcttcccaatttgcagtactt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAATGCTTCAGGGACAGTGGCCACC 254 183 1850 Antisense
TTCGCCGTTCTCGCAAATGACATAA 51 697 1891 Antisense
GACATAATGCGAGTACCGGCACCAC 432 369 1909 Antisense
ATGTTTGCATCAACGCCACAGACTT 76 49 1948 Antisense
ATCCCAAGTGCAACTACCGCATTCA 113 39 2002 Antisense
GCACCAAACATTCCATTTCCTGAAG 313 315 2035 Antisense
AAATTCTAGTTGGGCACTCGGCACA 515 115 2061 Antisense
GTGAAGCCGCTGGCGGCACAACCTA 2 483 2149 Antisense
ACAACCTAAGAAGAACTCCCCAGCT 67 101 2166 Antisense
GACCGAGGATATTCTTGTTCCACCA 527 381 2322 Antisense
GACAGCTTCCCAATTTGCAGTACTT 198 369 2360 Antisense
Sequence Source Affymetrix Proprietary Database