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[192228_at] MSP (Major sperm protein) domain.

Description M199.2 /REP_DB=WormBase Gene ID /WP=CE20019 /TR=Q9NAP2 /GB=CAB70252.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=MSP (Major sperm protein) domain.
Cluster 6grp_high1_10_10 > 55      6grp_total > 45      4grp_total > 15      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 80
1 2 3 4 range
A 66.6
(3)
P 		57.3
(1.2)
A 		59
(1.5)
A 		35.9
(-3)
A 		31
B 45
(-0.4)
P 		24.6
(-3)
A 		25.8
(-2.8)
A 		71.2
(3)
A 		47
C 87.1
(1.5)
P 		33.3
(-3)
A 		62.6
(-0.5)
P 		104.9
(3)
P 		72
avr 66.2
(2.2) 		38.4
(-3) 		49.1
(-1) 		70.7
(3) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 98
1 2 3 4 5 6 range
A 138.1
(2.5)
P 		85.1
(-1.4)
P 		145.5
(3)
P 		63.3
(-3)
P 		74.4
(-2.2)
M 		96
(-0.6)
P 		82
B 110.5
(0.7)
P 		110.5
(0.7)
P 		126.7
(3)
P 		91.6
(-1.9)
P 		83.7
(-3)
P 		122.7
(2.4)
P 		43
C 85.1
(0)
P 		96.4
(0.9)
P 		63.7
(-1.7)
P 		99.4
(1.2)
P 		47.1
(-3)
P 		122.2
(3)
P 		75
avr 111.2
(2.7) 		97.3
(0.8) 		112
(2.8) 		84.8
(-0.8) 		68.4
(-3) 		113.6
(3) 		45
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 24.3
(3)
P 		12.4
(-0.8)
A 		15.4
(0.2)
A 		5.3
(-3)
A 		19
B 14.3
(2.4)
P 		6.1
(-2.9)
A 		6
(-3)
A 		15.2
(3)
A 		9
C 28.8
(3)
P 		9.6
(-3)
A 		21.8
(0.8)
P 		17.7
(-0.5)
P 		19
avr 22.5
(3) 		9.4
(-3) 		14.4
(-0.7) 		12.7
(-1.5) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 31
1 2 3 4 5 6 range
A 25.5
(0.6)
P 		30.3
(2.5)
P 		31.7
(3)
P 		16
(-3)
P 		22.5
(-0.5)
M 		23.4
(-0.2)
P 		16
B 38.4
(0.9)
P 		36.8
(0.5)
P 		30.8
(-1.2)
P 		24.3
(-3)
P 		34
(-0.3)
P 		45.8
(3)
P 		22
C 31.5
(0.8)
P 		29.4
(0.3)
P 		16.2
(-2.8)
P 		20
(-1.9)
P 		15.2
(-3)
P 		40.9
(3)
P 		26
avr 31.8
(1.2) 		32.2
(1.4) 		26.2
(-0.8) 		20.1
(-3) 		23.9
(-1.6) 		36.7
(3) 		17
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192228_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10330
Sequence Type Exemplar sequence
Representative Public ID M199.2 NCBI
Target Description M199.2 /REP_DB=WormBase Gene ID /WP=CE20019 /TR=Q9NAP2 /GB=CAB70252.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=MSP (Major sperm protein) domain.
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070423(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070423 NCBI Caenorhabditis elegans M199.2 (M199.2) mRNA, complete cds. 11/11 None
SNAP00000027564 ENSEMBL cdna:SNAP chromosome:CEL140:IV:15108737:15110740:1 10/11 None
GENEFINDER00000027580 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:15108737:15110740:1 11/11 None
M199.2 ENSEMBL cdna:known chromosome:CEL140:IV:15108737:15110877:1 gene:M199.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:15108880-15110746(+) 100.0 100.0
Public Domain and Genome References
Gene Title MSP (Major sperm protein) domain.
Ensembl M199.2
Entrez Gene 178422 Entrez gene
SwissProt Q9NAP2 EMBL-EBI
WormBase CE20019 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB70252 Hypothetical protein M199.2 [Caenorhabditis elegans] ref|NP_502824.1| M199.2 [Caenorhabditis elegans] 0.0
blast CAB54365 Hypothetical protein Y105C5B.19 [Caenorhabditis elegans] ref|NP_502908.1| Y105C5B.19 [Caenorhabditis elegans] 0.0
blast CAB05725 Hypothetical protein F43D2.2 [Caenorhabditis elegans] ref|NP_506617.1| F43D2.2 [Caenorhabditis elegans] 1.0E-71
blast CAB05725 Hypothetical protein F43D2.2 [Caenorhabditis elegans] ref|NP_506617.1| F43D2.2 [Caenorhabditis elegans] 3.0E-71
Protein Domains
Database ID Description E-Value
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 1.3E-16
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 1.3E-16
Sequence
Target Sequence 
>C. ELEGANS:192228_AT
atcacaggcgccatagtgcagaacaagatgttttcgattgaaaccgcagagatttgggac
agagttttgacattgcccaatagaaaatggttccaaatttctgatgtagtgacagatgga
gaggtttcgaattggtcgtcggcaaatttggtgactcatgtgagagccctggcaagatcg
gttttcgataaggttgacgaagcggatccccatatgatgtgctacgcaacttcgcaaaac
gcttgcatatatgttcccattgataaggattttttcgatggatttgcagaatacctaatt
ggcggattcggccaagaacaatcgcgagatttgaagcttcaagtcagaaaactggtcacc
gaggttatgggaaataaactaaaccgtgtgagagcccgctataacaaaatgtatgggaaa
gatgcaagtgaggaagctgcaaaatggatgagagaggagcttgacaagaaggatggagaa
ctgaaagaatgtcaactcatattctcgaaaggc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCACAGGCGCCATAGTGCAGAACA 487 27 730 Antisense
GGACAGAGTTTTGACATTGCCCAAT 693 523 786 Antisense
GAGAGGTTTCGAATTGGTCGTCGGC 629 393 848 Antisense
GGTCGTCGGCAAATTTGGTGACTCA 367 503 863 Antisense
GAGAGCCCTGGCAAGATCGGTTTTC 624 393 891 Antisense
TAAGGTTGACGAAGCGGATCCCCAT 180 635 918 Antisense
TACGCAACTTCGCAAAACGCTTGCA 477 649 952 Antisense
AACGCTTGCATATATGTTCCCATTG 548 145 967 Antisense
GGCGGATTCGGCCAAGAACAATCGC 231 541 1030 Antisense
TAAACCGTGTGAGAGCCCGCTATAA 227 639 1109 Antisense
ATGTCAACTCATATTCTCGAAAGGC 183 49 1218 Antisense
Sequence Source Affymetrix Proprietary Database