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[192226_s_at] vesicular-fusion protein NSF

Description H15N14.1 /REP_DB=WormBase Gene ID /WP=CE19925 /TR=SW:Q94392 /GB=CAB01976.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=vesicular-fusion protein NSF
Cluster 6grp_highest_10_10 > 71      6grp_high1_10_10 > 17      4grp_highest_8_8 > 49      4grp_high_8_8 > 41      6grp_highest_nonorm > 10      6grp_total > 17      4grp_total > 54      6grp_highest_sub > De      6grp_cgc > 11      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1060
1 2 3 4 range
A 2050.9
(3)
P 		1369.1
(-0.9)
P 		1344.1
(-1)
P 		991.4
(-3)
P 		1060
B 1763
(3)
P 		1205.7
(-2)
P 		1219.7
(-1.9)
P 		1095.4
(-3)
P 		668
C 1745
(3)
P 		1385.2
(-0.5)
P 		1340.7
(-0.9)
P 		1126.7
(-3)
P 		618
avr 1853
(3) 		1320
(-1.1) 		1301.5
(-1.2) 		1071.2
(-3) 		782

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 931
1 2 3 4 5 6 range
A 1811.4
(3)
P 		1483.5
(0.1)
P 		1141.9
(-3)
P 		1213.1
(-2.4)
P 		1330.2
(-1.3)
P 		1375.8
(-0.9)
P 		670
B 2072.5
(3)
P 		1249
(-2.3)
P 		1415
(-1.2)
P 		1375.8
(-1.5)
P 		1142.9
(-3)
P 		1333
(-1.8)
P 		930
C 1802
(3)
P 		1602
(1)
P 		1450.4
(-0.6)
P 		1257.4
(-2.6)
P 		1214.1
(-3)
P 		1281.6
(-2.3)
P 		588
avr 1895.3
(3) 		1444.8
(-1.1) 		1335.8
(-2) 		1282.1
(-2.5) 		1229.1
(-3) 		1330.1
(-2.1) 		666
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 602
1 2 3 4 range
A 746.7
(3)
P 		297.2
(-1.5)
P 		351.4
(-0.9)
P 		145.1
(-3)
P 		602
B 562.2
(3)
P 		297.8
(-1.8)
P 		282.6
(-2.1)
P 		233.4
(-3)
P 		329
C 577.6
(3)
P 		398.3
(0.2)
P 		467.3
(1.3)
P 		190
(-3)
P 		388
avr 628.8
(3) 		331.1
(-1.1) 		367.1
(-0.6) 		189.5
(-3) 		439

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 472
1 2 3 4 5 6 range
A 333.9
(-1.2)
P 		527.6
(3)
P 		248.7
(-3)
P 		307.7
(-1.7)
P 		401.4
(0.3)
P 		335
(-1.1)
P 		279
B 721.1
(3)
P 		415.7
(-1.9)
P 		343.6
(-3)
P 		364.4
(-2.7)
P 		464.4
(-1.1)
P 		497.1
(-0.6)
P 		378
C 667.9
(3)
P 		488.8
(0.4)
P 		369.2
(-1.3)
P 		252.9
(-3)
P 		392.9
(-1)
P 		429
(-0.5)
P 		415
avr 574.3
(3) 		477.4
(0.8) 		320.5
(-2.7) 		308.3
(-3) 		419.6
(-0.5) 		420.4
(-0.5) 		266
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192226_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2050
Sequence Type Exemplar sequence
Representative Public ID H15N14.1 NCBI
Target Description H15N14.1 /REP_DB=WormBase Gene ID /WP=CE19925 /TR=SW:Q94392 /GB=CAB01976.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=vesicular-fusion protein NSF
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_170902(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_170902 NCBI Caenorhabditis elegans NSF (N-ethylmaleimide sensitive secretion factor) homolog family member (nsf-1) (nsf-1) mRNA, complete cds. 11/11 None
H15N14.2 ENSEMBL cdna:known chromosome:CEL140:I:7774662:7780334:-1 gene:H15N14.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:7774894-7780265(-) 100.0 100.0
Public Domain and Genome References
Gene Title vesicular-fusion protein NSF
Gene Symbol nsf-1
Ensembl H15N14.2
Entrez Gene 266842 Entrez gene
SwissProt Q94392 EMBL-EBI
WormBase CE19925 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1639023_AT NEM-sensitive fusion protein 2 dm
DROSGENOME1:143738_AT NEM-sensitive fusion protein 2 dm
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
166 nucleotide binding inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB01976 Hypothetical protein H15N14.2 [Caenorhabditis elegans] emb|CAB09531.1| Hypothetical protein H15N14.2 [Caenorhabditis elegans] ref|NP_740892.1| NSF (N-ethylmaleimide sensitive secretion factor) homolog family member (nsf-1) [Caenorhabditis elegans] sp|Q94392|NSF_CAEEL Vesicle-fusing ATPase (Vesicular-fusion protein NSF) (N-ethylmaleimide sensitive fusion protein) (NEM-sensitive fusion protein) 0.0
blast CAE72309 Hypothetical protein CBG19441 [Caenorhabditis briggsae] 0.0
blast AAW58140 N-ethylmaleimide sensitive fusion protein [Helicoverpa armigera] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004201 EMBL-EBI Cell division protein 48, CDC48, domain 2 5.4E-28
Pfam IPR003959 EMBL-EBI AAA ATPase, central region 3.3E-79
Pfam IPR003338 EMBL-EBI AAA ATPase VAT, N-terminal 1.1E-32
Sequence
Target Sequence 
>C. ELEGANS:192226_S_AT
tcggacttcccattcgtcaaagttatatcaccagaagatactgttggattctcagaatct
gcgaagtgtatggcgttgaagaaagcttttgaagatgcgaaacgatcaaagctcagtgtt
cttctaatcgataatttggaaagattgatcgactaccatccggtcggcccacgatactcc
aatctggtaattcaagctcttctcgtacttctcaatgctcctccaccggccggtcaccgt
ctgttcgtcatcgcaacctcatcggatcgtatgttcttaagagacatgggacttatggat
gtatttggagatgttattgatattcctaaactcactacagcaggacaaatgatgaatgtg
attcaggagagtaacatttattccgatgatcaacttcctatgattgaacaaaaacttgca
tcaatatgtagaggagaaggattccatggagtcggtatcaaacatcttctcgaattaatt
gaatctgctcgtcagtgtgaagccgattaccgcgtcccgacacttcttaatatgatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGGACTTCCCATTCGTCAAAGTTA 83 599 1921 Antisense
AGCTCAGTGTTCTTCTAATCGATAA 194 75 2030 Antisense
AAGATTGATCGACTACCATCCGGTC 539 155 2061 Antisense
GCCCACGATACTCCAATCTGGTAAT 124 287 2087 Antisense
GTAATTCAAGCTCTTCTCGTACTTC 501 463 2107 Antisense
ATCGCAACCTCATCGGATCGTATGT 366 37 2170 Antisense
GATATTCCTAAACTCACTACAGCAG 505 427 2239 Antisense
TATTCCGATGATCAACTTCCTATGA 382 663 2299 Antisense
GAGTCGGTATCAAACATCTTCTCGA 627 399 2369 Antisense
GTCAGTGTGAAGCCGATTACCGCGT 462 465 2411 Antisense
CGTCCCGACACTTCTTAATATGATG 705 245 2433 Antisense
Sequence Source Affymetrix Proprietary Database