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[192221_at]

Description C25B8.1 /REP_DB=WormBase Gene ID /WP=CE27086 /GEN=klq-1 /GB=AAK39192.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=voltage-gated potassium channel
Cluster 6grp_total > 34      4grp_total > 47      6grp_cgc > 21      6grp_cgc_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 162
1 2 3 4 range
A 129.6
(3)
P 		71.4
(-0.2)
A 		88.3
(0.7)
A 		19.6
(-3)
A 		110
B 172.5
(3)
P 		74.6
(-2.3)
A 		61.6
(-3)
A 		76.3
(-2.2)
A 		111
C 182
(3)
P 		75.4
(-2.5)
A 		65.1
(-3)
A 		111.9
(-0.6)
A 		117
avr 161.4
(3) 		73.8
(-2.7) 		71.7
(-2.8) 		69.3
(-3) 		92

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 123
1 2 3 4 5 6 range
A 176.4
(3)
M 		99.9
(-1)
M 		127.1
(0.4)
P 		63
(-3)
A 		101.7
(-1)
A 		69.4
(-2.7)
A 		113
B 157.1
(3)
P 		123.9
(0.9)
A 		109.7
(0.1)
A 		92.6
(-1)
A 		60.4
(-3)
A 		82.6
(-1.6)
A 		97
C 124.2
(3)
P 		98.5
(0.8)
A 		111.9
(2)
M 		53.7
(-3)
A 		63.5
(-2.2)
A 		62.2
(-2.3)
A 		71
avr 152.6
(3) 		107.4
(-0.3) 		116.2
(0.4) 		69.8
(-3) 		75.2
(-2.6) 		71.4
(-2.9) 		83
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 57
1 2 3 4 range
A 47.2
(3)
P 		15.5
(-1.3)
A 		23.1
(-0.3)
A 		2.9
(-3)
A 		44
B 55
(3)
P 		18.4
(-2.4)
A 		14.3
(-3)
A 		16.3
(-2.7)
A 		41
C 60.2
(3)
P 		21.7
(-2.6)
A 		22.7
(-2.4)
A 		18.9
(-3)
A 		41
avr 54.1
(3) 		18.5
(-2.2) 		20
(-1.9) 		12.7
(-3) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 44
1 2 3 4 5 6 range
A 32.5
(2.1)
M 		35.5
(3)
M 		27.7
(0.6)
P 		16
(-3)
A 		30.7
(1.5)
A 		16.9
(-2.7)
A 		20
B 54.7
(3)
P 		41.2
(0.3)
A 		26.6
(-2.6)
A 		24.5
(-3)
A 		24.5
(-3)
A 		30.8
(-1.7)
A 		30
C 46.1
(3)
P 		30.1
(0.3)
A 		28.5
(0)
M 		10.8
(-3)
A 		20.5
(-1.4)
A 		20.8
(-1.3)
A 		35
avr 44.4
(3) 		35.6
(1.1) 		27.6
(-0.7) 		17.1
(-3) 		25.2
(-1.2) 		22.8
(-1.7) 		27
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192221_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16936
Sequence Type Exemplar sequence
Representative Public ID C25B8.1 NCBI
Target Description C25B8.1 /REP_DB=WormBase Gene ID /WP=CE27086 /GEN=klq-1 /GB=AAK39192.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=voltage-gated potassium channel
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171709(10), NM_171710(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171709 NCBI Caenorhabditis elegans potassium channel, KvQLT family member (kqt-1) (kqt-1) mRNA, complete cds. 10/11 None
NM_171710 NCBI Caenorhabditis elegans potassium channel, KvQLT family member (kqt-1) (kqt-1) mRNA, complete cds. 10/11 None
GENEFINDER00000010951 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:6618977:6627659:1 9/11 None
SNAP00000010944 ENSEMBL cdna:SNAP chromosome:CEL140:X:6625312:6627659:1 9/11 None
C25B8.1b ENSEMBL cdna:known chromosome:CEL140:X:6618977:6627930:1 gene:C25B8.1 10/11 A 
C25B8.1a ENSEMBL cdna:known chromosome:CEL140:X:6619866:6627659:1 gene:C25B8.1 9/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:6620034-6627995(+) 100.0 100.0
Public Domain and Genome References
Ensembl C25B8.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM51518 Potassium channel, kvqlt family protein 1, isoform b [Caenorhabditis elegans] ref|NP_741819.1| potassium channel, KvQLT family member (kqt-1) [Caenorhabditis elegans] 0.0
blast AAK39192 Potassium channel, kvqlt family protein 1, isoform a [Caenorhabditis elegans] ref|NP_741820.1| potassium channel, KvQLT family member (kqt-1) [Caenorhabditis elegans] gb|AAT77227.1| KQT-1 potassium channel [Caenorhabditis elegans] 0.0
blast T34116 voltage-gated potassium channel klq-1 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR005821 EMBL-EBI Ion transport protein 2.9E-32
Pfam IPR003937 EMBL-EBI KCNQ voltage-gated potassium channel 2.1E-19
Pfam IPR005821 EMBL-EBI Ion transport protein 2.9E-32
Pfam IPR003937 EMBL-EBI KCNQ voltage-gated potassium channel 2.1E-19
Pfam IPR005821 EMBL-EBI Ion transport protein 2.9E-32
Pfam IPR003937 EMBL-EBI KCNQ voltage-gated potassium channel 2.1E-19
Pfam IPR003937 EMBL-EBI KCNQ voltage-gated potassium channel 2.1E-19
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_741819.1 5 149-171,181-203,224-246,288-310,349-371
NP_741820.1 5 101-123,133-155,176-198,240-262,301-323
C25B8.1b 5 149-171,181-203,224-246,288-310,349-371
C25B8.1a 5 101-123,133-155,176-198,240-262,301-323
GENEFINDER00000010951 5 168-190,200-222,243-265,307-329,368-390
Sequence
Target Sequence 
>C. ELEGANS:192221_AT
gaatacaactcttggtggctcggaagaaattcaaggaggcattaaaaccctatgacgtca
aagatgtgattgaacaatattcagctggtcatgttgatttgcaaagtcgagtgaagacag
ttcaagcgaaactagattttatatgcgggaaaaatattgagaaaattgagccaaaaattt
caatgttcactagaattgcaactttggaaacaactgttggtaaaatggacaaaaagctcg
acttaatggttgaaatgctaatgggaaggcaagcatcccagcgtgtcttctcacagaaca
catcgccacgtggagagttttctgaaccaactagtgcgagacaagatttgacaaggtcgc
ggcggtctatggtcagcactgacatggaaatgtatacagcaagatcacattcgcctggtt
atcatggagatgcccgaccaattattgctcaaatagatgcagatgatgacgatgaagatg
agaatgttttcgacgattctactccattgaacaatggtcccgttagtgttttcacgcttt
tcaaattctacca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATACAACTCTTGGTGGCTCGGAA 608 325 1622 Antisense
GGAGGCATTAAAACCCTATGACGTC 681 513 1656 Antisense
TGGGAAGGCAAGCATCCCAGCGTGT 200 551 1883 Antisense
TGTCTTCTCACAGAACACATCGCCA 7 559 1905 Antisense
CGCCACGTGGAGAGTTTTCTGAACC 214 257 1925 Antisense
GACAAGGTCGCGGCGGTCTATGGTC 275 363 1971 Antisense
GGCGGTCTATGGTCAGCACTGACAT 456 539 1982 Antisense
GCAAGATCACATTCGCCTGGTTATC 209 321 2020 Antisense
GGAGATGCCCGACCAATTATTGCTC 91 517 2047 Antisense
ATGGTCCCGTTAGTGTTTTCACGCT 352 51 2135 Antisense
TTTTCACGCTTTTCAAATTCTACCA 631 675 2150 Antisense
Sequence Source Affymetrix Proprietary Database