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[192215_s_at]

Description C44C11.1 /REP_DB=WormBase Gene ID /WP=CE24846 /GEN=ras-1 /TR=O45056 /GB=AAK72295.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=RAS-related protein
Cluster 6grp_highest_10_10 > 60      6grp_high1_10_10 > 06      4grp_highest_8_8 > 60      4grp_high_8_8 > 25      6grp_highest_nonorm > 70      6grp_total > 07      4grp_total > 38      6grp_highest_sub > De      6grp_cgc > 11      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 468
1 2 3 4 range
A 569.4
(3)
P 		219.7
(-3)
P 		227.2
(-2.9)
P 		410
(0.3)
P 		350
B 516.3
(3)
P 		318.4
(-1.3)
P 		239.7
(-3)
P 		407.9
(0.6)
P 		277
C 688.1
(3)
P 		479.2
(-0.2)
P 		298.2
(-3)
P 		335.7
(-2.4)
P 		390
avr 591.3
(3) 		339.1
(-1.5) 		255
(-3) 		384.5
(-0.7) 		336

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 311
1 2 3 4 5 6 range
A 319.5
(2.4)
P 		333
(3)
P 		251.3
(-0.5)
P 		243.7
(-0.8)
P 		193.5
(-3)
P 		208.2
(-2.4)
P 		140
B 496.1
(3)
P 		260.9
(-1.5)
P 		255.7
(-1.6)
P 		185.4
(-3)
P 		249.4
(-1.8)
P 		221
(-2.3)
P 		311
C 315.5
(3)
P 		266.7
(-1.3)
P 		305.8
(2.1)
P 		289.4
(0.7)
P 		247.6
(-3)
P 		266.5
(-1.3)
P 		68
avr 377
(3) 		286.9
(-0.7) 		270.9
(-1.3) 		239.5
(-2.6) 		230.2
(-3) 		231.9
(-2.9) 		147
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 180
1 2 3 4 range
A 207.3
(3)
P 		47.7
(-3)
P 		59.4
(-2.6)
P 		60
(-2.5)
P 		160
B 164.6
(3)
P 		78.6
(-1.7)
P 		55.5
(-3)
P 		86.9
(-1.3)
P 		109
C 227.8
(3)
P 		137.8
(-0.2)
P 		103.9
(-1.3)
P 		56.6
(-3)
P 		171
avr 199.9
(3) 		88
(-2.1) 		72.9
(-2.8) 		67.8
(-3) 		132

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 124
1 2 3 4 5 6 range
A 58.9
(-2.3)
P 		118.4
(3)
P 		54.7
(-2.6)
P 		61.8
(-2)
P 		58.4
(-2.3)
P 		50.7
(-3)
P 		68
B 172.6
(3)
P 		86.8
(-1.2)
P 		62.1
(-2.4)
P 		49.1
(-3)
P 		101.3
(-0.5)
P 		82.4
(-1.4)
P 		124
C 116.9
(3)
P 		81.4
(-0.6)
P 		77.8
(-1)
P 		58.2
(-3)
P 		80.1
(-0.8)
P 		89.2
(0.2)
P 		59
avr 116.1
(3) 		95.5
(0.9) 		64.9
(-2.1) 		56.4
(-3) 		79.9
(-0.6) 		74.1
(-1.2) 		60
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192215_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3879
Sequence Type Exemplar sequence
Representative Public ID C44C11.1 NCBI
Target Description C44C11.1 /REP_DB=WormBase Gene ID /WP=CE24846 /GEN=ras-1 /TR=O45056 /GB=AAK72295.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=RAS-related protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064222(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064222 NCBI Caenorhabditis elegans R-RAS related family member (ras-1) (ras-1) mRNA, complete cds. 11/11 A 
GENEFINDER00000019446 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:12345613:12352126:-1 11/11 None
C44C11.1a ENSEMBL cdna:known chromosome:CEL140:II:12345613:12352053:-1 gene:C44C11.1 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001026858 7/11 Cross Hyb Matching Probes None
SNAP00000019444 4/11 Cross Hyb Matching Probes None
SNAP00000019445 5/11 Cross Hyb Matching Probes None
C44C11.1b.1 7/11 Cross Hyb Matching Probes None
C44C11.1b.2 7/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
174875 2 1 NM_064222 192215_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:12345609-12352050(-) 100.0 100.0
Public Domain and Genome References
Ensembl C44C11.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK72295 R-ras related protein 1, isoform a [Caenorhabditis elegans] ref|NP_496623.1| R-RAS related family member (ras-1) [Caenorhabditis elegans] gb|AAB66712.1| GTP binding protein [Caenorhabditis elegans] gb|AAB03320.1| R-ras1 homolog 1.0E-108
blast CAE73486 Hypothetical protein CBG20938 [Caenorhabditis briggsae] 1.0E-104
blast T32953 hypothetical protein C44C11.1 - Caenorhabditis elegans 4.0E-91
blast T32953 hypothetical protein C44C11.1 - Caenorhabditis elegans 6.0E-91
Protein Domains
Database ID Description E-Value
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 6.1E-88
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 6.1E-88
Sequence
Target Sequence 
>C. ELEGANS:192215_S_AT
atgcagtgctccgaatcgttgtcgtcggcggtggaggtgtcgggaaatcggcgttgacca
tacagtttatacagaggtactttgtgcaggactacgacccgacgatcgaagactcctaca
ccaaacaatgtttcgtcgacgaggatctttgcaaactggaaatccttgacactgctggac
aggaggagttttccacgatgcgagagcagtatttgcgaacggggagcggttttttaattg
tgtttgcagttactgacaggaatagtttcgaagaggtcaaaaagctacacgaattaatat
gccgaatcaaggatcgagatgattttccaattatactagtgggcaataaagcagatttgg
aaaatgagagacacgtagcccgacatgaagccgaggagctcgcacaccgactctcaatac
catatttggagtgctcggcaaaaattcgaaaaaatgttgatgaagcgttttttgatattg
tgagattagtcagaaaataccaacatgacgagcgcatgcccatccacccccatgacgatc
ggaagctcgaatcaccgatcaagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCAGTGCTCCGAATCGTTGTCGT 259 43 56 Antisense
GAAATCGGCGTTGACCATACAGTTT 359 361 99 Antisense
GGTACTTTGTGCAGGACTACGACCC 43 1 131 Antisense
ACTACGACCCGACGATCGAAGACTC 345 101 146 Antisense
TCGAAGACTCCTACACCAAACAATG 481 601 161 Antisense
GTCGACGAGGATCTTTGCAAACTGG 659 473 190 Antisense
GAGTTTTCCACGATGCGAGAGCAGT 78 399 241 Antisense
GTAGCCCGACATGAAGCCGAGGAGC 514 457 430 Antisense
CACACCGACTCTCAATACCATATTT 517 195 458 Antisense
ACCAACATGACGAGCGCATGCCCAT 40 85 554 Antisense
CGGAAGCTCGAATCACCGATCAAGC 697 241 595 Antisense
Sequence Source Affymetrix Proprietary Database