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[192212_at]

Description C14A11.7 /REP_DB=WormBase Gene ID /WP=CE08155 /GEN=ssr-2 /TR=O02139 /GB=AAB52874.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=RAS-related protein
Cluster 6grp_total > 48      4grp_total > 36      6grp_cgc > 17      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 150
1 2 3 4 range
A 182.9
(1.8)
P 		204.3
(3)
A 		97.3
(-3)
A 		142.1
(-0.5)
P 		107
B 193.3
(-2.9)
P 		247
(3)
P 		192.4
(-3)
A 		221.5
(0.2)
P 		55
C 192.7
(2.4)
P 		191.4
(2.2)
A 		151.5
(-3)
A 		197.2
(3)
P 		46
avr 189.6
(0.8) 		214.2
(3) 		147.1
(-3) 		186.9
(0.6) 		67

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 224
1 2 3 4 5 6 range
A 243.1
(3)
P 		83.6
(-1.3)
P 		117.4
(-0.4)
P 		45.5
(-2.3)
A 		19.1
(-3)
A 		187.1
(1.5)
A 		224
B 110.8
(1.1)
P 		107.2
(0.1)
A 		95.2
(-3)
P 		114.2
(2)
P 		115.7
(2.4)
M 		118.1
(3)
P 		23
C 89.1
(1.2)
P 		87.3
(1)
P 		53.8
(-3)
P 		94.2
(1.8)
A 		86.7
(0.9)
A 		103.9
(3)
A 		50
avr 147.7
(3) 		92.7
(-1.5) 		88.8
(-1.8) 		84.6
(-2.1) 		73.8
(-3) 		136.4
(2.1) 		74
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 46
1 2 3 4 range
A 66.6
(3)
P 		44.4
(0.1)
A 		25.4
(-2.4)
A 		20.8
(-3)
P 		46
B 61.7
(3)
P 		61
(2.8)
P 		44.6
(-3)
A 		47.2
(-2.1)
P 		17
C 63.8
(3)
P 		55
(1.3)
A 		52.8
(0.8)
A 		33.2
(-3)
P 		31
avr 64
(3) 		53.5
(0.9) 		40.9
(-1.6) 		33.7
(-3) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 41
1 2 3 4 5 6 range
A 44.8
(2.9)
P 		29.7
(0.6)
P 		25.6
(-0)
P 		11.5
(-2.1)
A 		5.7
(-3)
A 		45.6
(3)
A 		40
B 38.6
(0.9)
P 		35.7
(0.2)
A 		23.1
(-3)
P 		30.3
(-1.2)
P 		47
(3)
M 		44.1
(2.3)
P 		24
C 33
(2.5)
P 		26.6
(0.7)
P 		13.7
(-3)
P 		18.9
(-1.5)
A 		28.1
(1.1)
A 		34.8
(3)
A 		21
avr 38.8
(2.2) 		30.7
(-0) 		20.8
(-2.8) 		20.2
(-3) 		26.9
(-1.1) 		41.5
(3) 		21
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192212_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18371
Sequence Type Exemplar sequence
Representative Public ID C14A11.7 NCBI
Target Description C14A11.7 /REP_DB=WormBase Gene ID /WP=CE08155 /GEN=ssr-2 /TR=O02139 /GB=AAB52874.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=RAS-related protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076089
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000039994 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:2823572:2825723:-1 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_076089 6/11 Cross Hyb Matching Probes None
SNAP00000039988 5/11 Cross Hyb Matching Probes None
C14A11.7 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:2823587-2826840(-) 88.89 100.0
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE58828 Hypothetical protein CBG02039 [Caenorhabditis briggsae] 1.0E-89
blast AAB52874 Sperm-specific family, class r protein 2 [Caenorhabditis elegans] ref|NP_508490.2| Sperm-Specific family, class R family member (ssr-2) [Caenorhabditis elegans] 4.0E-88
blast AAF57410 CG30158-PA [Drosophila melanogaster] ref|NP_652315.2| CG30158-PA [Drosophila melanogaster] 1.0E-17
Protein Domains
Database ID Description E-Value
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 3.3E-20
Sequence
Target Sequence 
>C. ELEGANS:192212_AT
tgcgggaatgcatgctagttcagctaagttacgacgtttcaaaaataaaaaatctcctct
tgaaaaaaaaaattatttgtaaaaaaattgcttttttgaatattgataaaaatacttctg
aaaagttagccggatgtgatgtaccattttttgaagtactatactcttatacgtttgcat
ttcaggatgagaaaaacacagcttctacaaaaaacggctcacaatctgctcgtgagcaac
ctcaacttgataagcaggcttctcaccccgttcttgcagtcccgctgcacattggcgatt
tgaaaagaaacctttctttaagagtatgtatcaatccctatatgaagtcaccgcgtaggg
acaaagaacaagtcgagaggaagctgtcggatgagcctaaaatttcacgcagctccagtc
ttatccggtttgaaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCGGGAATGCATGCTAGTTCAGCT 638 581 740 Antisense
AGTTCAGCTAAGTTACGACGTTTCA 453 63 756 Antisense
TACTCTTATACGTTTGCATTTCAGG 134 647 901 Antisense
AAAAACGGCTCACAATCTGCTCGTG 351 129 949 Antisense
CTGCTCGTGAGCAACCTCAACTTGA 395 237 965 Antisense
ACTTGATAAGCAGGCTTCTCACCCC 231 105 984 Antisense
TCCCGCTGCACATTGGCGATTTGAA 140 597 1019 Antisense
CAATCCCTATATGAAGTCACCGCGT 599 181 1071 Antisense
GAGGAAGCTGTCGGATGAGCCTAAA 258 405 1116 Antisense
GAGCCTAAAATTTCACGCAGCTCCA 478 385 1132 Antisense
GCTCCAGTCTTATCCGGTTTGAAAG 413 295 1151 Antisense
Sequence Source Affymetrix Proprietary Database