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[192208_s_at]

Description F33E2.2 /REP_DB=WormBase Gene ID /WP=CE23702 /TR=O01700 /GB=CAB06544.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Eukaryotic protein kinase domain
Cluster 6grp_highest_10_10 > 40      6grp_high1_10_10 > 05      4grp_highest_8_8 > 54      4grp_high_8_8 > 10      6grp_highest_nonorm > 30      6grp_total > 14      4grp_total > 22      6grp_highest_sub > De      6grp_cgc > 31      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 735
1 2 3 4 range
A 1245.7
(2.8)
P 		813
(-1.1)
P 		596.1
(-3)
P 		1265.5
(3)
P 		669
B 1303.9
(2.9)
P 		630.9
(-2.9)
P 		616
(-3)
P 		1320
(3)
P 		704
C 1153.7
(2.4)
P 		707.5
(-1.8)
P 		585.2
(-3)
P 		1217.8
(3)
P 		633
avr 1234.4
(2.7) 		717.1
(-1.9) 		599.1
(-3) 		1267.8
(3) 		669

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1013
1 2 3 4 5 6 range
A 1308
(3)
P 		984.1
(0.8)
P 		666.3
(-1.3)
P 		623.8
(-1.6)
P 		587.3
(-1.8)
P 		412
(-3)
P 		896
B 1263.6
(3)
P 		1033.4
(1.3)
P 		772.9
(-0.6)
P 		722.5
(-1)
P 		546.2
(-2.3)
P 		449.4
(-3)
P 		814
C 1344.3
(3)
P 		1018.3
(1.1)
P 		833.2
(-0)
P 		838.5
(0)
P 		654.8
(-1.1)
P 		331.5
(-3)
P 		1013
avr 1305.3
(3) 		1011.9
(1.1) 		757.5
(-0.6) 		728.3
(-0.8) 		596.1
(-1.7) 		397.6
(-3) 		908
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 311
1 2 3 4 range
A 453.6
(3)
P 		176.5
(-2.6)
P 		155.8
(-3)
P 		185.2
(-2.4)
P 		298
B 415.8
(3)
P 		155.8
(-2.7)
P 		142.7
(-3)
P 		281.3
(0)
P 		273
C 381.9
(3)
P 		203.4
(-3)
P 		204
(-3)
P 		205.3
(-2.9)
P 		179
avr 417.1
(3) 		178.6
(-2.7) 		167.5
(-3) 		223.9
(-1.6) 		250

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 398
1 2 3 4 5 6 range
A 241.1
(0.4)
P 		350
(3)
P 		145.1
(-1.9)
P 		158.2
(-1.6)
P 		177.2
(-1.2)
P 		100.3
(-3)
P 		250
B 439.7
(3)
P 		344
(0.9)
P 		187.7
(-2.6)
P 		191.4
(-2.5)
P 		221.9
(-1.8)
P 		167.6
(-3)
P 		272
C 498.3
(3)
P 		310.7
(0.1)
P 		212.1
(-1.4)
P 		168.6
(-2.1)
P 		211.9
(-1.4)
P 		111
(-3)
P 		387
avr 393
(3) 		334.9
(1.7) 		181.6
(-1.8) 		172.7
(-2) 		203.7
(-1.3) 		126.3
(-3) 		267
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192208_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1693
Sequence Type Exemplar sequence
Representative Public ID F33E2.2 NCBI
Target Description F33E2.2 /REP_DB=WormBase Gene ID /WP=CE23702 /TR=O01700 /GB=CAB06544.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Eukaryotic protein kinase domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026273(11), NM_001026272(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026273 NCBI Caenorhabditis elegans DAP (Death Associated Protein kinase) Like Kinase family member (dlk-1) (dlk-1) mRNA, complete cds. 11/11 None
NM_001026272 NCBI Caenorhabditis elegans DAP (Death Associated Protein kinase) Like Kinase family member (dlk-1) (dlk-1) mRNA, complete cds. 11/11 None
F33E2.2b ENSEMBL cdna:known chromosome:CEL140:I:12573917:12580917:-1 gene:F33E2.2 11/11 None
F33E2.2a ENSEMBL cdna:known chromosome:CEL140:I:12574250:12580917:-1 gene:F33E2.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
173128 3 2 NM_001026272 192208_s_at (11)
NM_001026273 173354_s_at (11), 192208_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:12574180-12578916(-) 100.0 100.0
Public Domain and Genome References
Ensembl F33E2.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE54891 Hypothetical protein F33E2.2b [Caenorhabditis elegans] emb|CAE54889.1| Hypothetical protein F33E2.2b [Caenorhabditis elegans] ref|NP_001021444.1| DAP (Death Associated Protein kinase) Like Kinase family member (dlk-1) [Caenorhabditis elegans] 0.0
blast CAB06544 Hypothetical protein F33E2.2a [Caenorhabditis elegans] emb|CAA18635.3| Hypothetical protein F33E2.2a [Caenorhabditis elegans] ref|NP_001021443.1| DAP (Death Associated Protein kinase) Like Kinase family member (dlk-1) [Caenorhabditis elegans] 0.0
blast CAE64099 Hypothetical protein CBG08707 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
hanks 2.1.13 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; ACMPK 4.0E-63
hanks 2.1.13 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; ACMPK 3.0E-63
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 2.4E-69
Sequence
Target Sequence 
>C. ELEGANS:192208_S_AT
ggagcccaactccatatgacaatgattttgaaaatgccgagagcttcgtggatccggaat
ctccgaaaaatttgaaaaatttggaaaaaatcgtaaacttgccagagtccacgtcctacg
acgaggctttatgcaatagtgatgtgacaatgaacccgatttacacgtcaccaatcacca
cctacagtaacccgtgccacgtggagctggtggacgaggaaaacgcaaatgacgtggatc
tgacgtcatcgatggattcccgacgatcccgctccgacgatgctgatgttgaatcttcgg
aagaagatgagggaaatggaaataatattctgaatacttctatggaatctgaggatcttc
gatatcgaattgacacgtctcagagtactatgatgagctcattggagagaagtctggaaa
ttggagccacccggtccgatgggcttagtgat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAGCCCAACTCCATATGACAATGA 608 521 1979 Antisense
AGAGCTTCGTGGATCCGGAATCTCC 638 67 2018 Antisense
CGTAAACTTGCCAGAGTCCACGTCC 247 247 2070 Antisense
ACGTCCTACGACGAGGCTTTATGCA 178 95 2089 Antisense
AATGAACCCGATTTACACGTCACCA 442 163 2127 Antisense
CAGTAACCCGTGCCACGTGGAGCTG 307 207 2163 Antisense
CGTGGATCTGACGTCATCGATGGAT 418 245 2211 Antisense
TCATCGATGGATTCCCGACGATCCC 298 621 2224 Antisense
CGCTCCGACGATGCTGATGTTGAAT 456 255 2248 Antisense
AGTCTGGAAATTGGAGCCACCCGGT 299 61 2389 Antisense
CACCCGGTCCGATGGGCTTAGTGAT 649 87 2406 Antisense
Sequence Source Affymetrix Proprietary Database