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[192207_at]

Description F13H8.2 /REP_DB=WormBase Gene ID /WP=CE27127 /TR=Q19433 /GB=AAK31492.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=beta transducin Trp-Asp domains
Cluster 6grp_highest_10_10 > 35      6grp_high1_10_10 > 30      4grp_highest_8_8 > 43      4grp_high_8_8 > 32      6grp_highest_nonorm > 71      6grp_total > 40      4grp_total > 55      6grp_highest_sub > 03      6grp_cgc > 82      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 228
1 2 3 4 range
A 350.8
(3)
P 		216.3
(-3)
P 		282.8
(-0)
P 		234.8
(-2.2)
P 		135
B 425.4
(3)
P 		243.2
(-2.5)
P 		225.4
(-3)
P 		249.4
(-2.3)
P 		200
C 392
(3)
P 		197.4
(-3)
P 		250.8
(-1.4)
P 		215
(-2.5)
P 		195
avr 389.4
(3) 		219
(-3) 		253
(-1.8) 		233.1
(-2.5) 		170

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 258
1 2 3 4 5 6 range
A 235
(-1.2)
P 		308.2
(0.5)
P 		403.6
(2.7)
P 		417.3
(3)
P 		158.9
(-3)
P 		194.3
(-2.2)
P 		258
B 328.4
(1.2)
P 		322.2
(1)
P 		385.4
(3)
P 		217.7
(-2.3)
P 		196.5
(-3)
P 		238.2
(-1.7)
P 		189
C 292.1
(0.4)
P 		372.5
(2.7)
P 		381.5
(3)
P 		297.5
(0.6)
P 		173
(-3)
P 		275.3
(-0.1)
P 		209
avr 285.2
(0.1) 		334.3
(1.4) 		390.2
(3) 		310.8
(0.8) 		176.1
(-3) 		235.9
(-1.3) 		214
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 101
1 2 3 4 range
A 127.7
(3)
P 		46.9
(-2.2)
P 		73.9
(-0.5)
P 		34.4
(-3)
P 		93
B 135.6
(3)
P 		60.1
(-2.4)
P 		52.2
(-3)
P 		53.2
(-2.9)
P 		83
C 129.7
(3)
P 		56.8
(-1.7)
P 		87.4
(0.3)
P 		36.2
(-3)
P 		94
avr 131
(3) 		54.6
(-2.1) 		71.2
(-1) 		41.3
(-3) 		90

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 71
1 2 3 4 5 6 range
A 43.3
(-3)
P 		109.6
(3)
P 		87.9
(1)
P 		105.8
(2.7)
P 		47.9
(-2.6)
P 		47.3
(-2.6)
P 		66
B 114.3
(3)
P 		107.3
(2.3)
P 		93.6
(0.8)
P 		57.7
(-3)
P 		79.8
(-0.7)
P 		88.8
(0.3)
P 		57
C 108.3
(2.4)
P 		113.7
(3)
P 		97.1
(1.3)
P 		59.8
(-2.6)
P 		56
(-3)
P 		92.2
(0.8)
P 		58
avr 88.6
(0.4) 		110.2
(3) 		92.9
(0.9) 		74.4
(-1.4) 		61.2
(-3) 		76.1
(-1.2) 		49
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192207_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4841
Sequence Type Exemplar sequence
Representative Public ID F13H8.2 NCBI
Target Description F13H8.2 /REP_DB=WormBase Gene ID /WP=CE27127 /TR=Q19433 /GB=AAK31492.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=beta transducin Trp-Asp domains
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062856(11), NM_182120(11), NM_182121(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062856 NCBI Caenorhabditis elegans Biotin Protein Ligase family member (bpl-1) (bpl-1) mRNA, complete cds. 11/11 None
NM_182120 NCBI Caenorhabditis elegans Biotin Protein Ligase family member (bpl-1) (bpl-1) mRNA, complete cds. 11/11 None
NM_182121 NCBI Caenorhabditis elegans Biotin Protein Ligase family member (bpl-1) (bpl-1) mRNA, complete cds. 11/11 None
SNAP00000031307 ENSEMBL cdna:SNAP chromosome:CEL140:II:6274213:6279600:1 11/11 None
GENEFINDER00000031314 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6270320:6279600:1 11/11 None
F13H8.10a ENSEMBL cdna:known chromosome:CEL140:II:6275206:6279662:1 gene:F13H8.10 11/11 None
F13H8.10b ENSEMBL cdna:known chromosome:CEL140:II:6277218:6279658:1 gene:F13H8.10 11/11 None
F13H8.10c.2 ENSEMBL cdna:known chromosome:CEL140:II:6277289:6279600:1 gene:F13H8.10 11/11 None
F13H8.10c.1 ENSEMBL cdna:known chromosome:CEL140:II:6277552:6279600:1 gene:F13H8.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6270316-6279597(+) 97.56 100.0
Public Domain and Genome References
Ensembl F13H8.10
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK31491 Biotin protein ligase protein 1, isoform a [Caenorhabditis elegans] ref|NP_495257.2| Biotin Protein Ligase family member (bpl-1) [Caenorhabditis elegans] 0.0
blast T16064 hypothetical protein F13H8.2 - Caenorhabditis elegans 0.0
blast CAE59280 Hypothetical protein CBG02612 [Caenorhabditis briggsae] 0.0
blast AAN65327 Biotin protein ligase protein 1, isoform b [Caenorhabditis elegans] ref|NP_871920.1| Biotin Protein Ligase family member (bpl-1) [Caenorhabditis elegans] gb|AAS98221.1| biotin protein ligase, holocarboxylase synthetase (70.9 kD) alternative variant b [Caenorhabditis elegans] 0.0
blast AAN65328 Biotin protein ligase protein 1, isoform c [Caenorhabditis elegans] ref|NP_871921.1| Biotin Protein Ligase family member (bpl-1) [Caenorhabditis elegans] gb|AAS98223.1| biotin protein ligase, holocarboxylase synthetase (62.5 kD) alternative variant d [Caenorhabditis elegans] gb|AAS98222.1| biotin protein ligase, holocarboxylase synthetase (62.5 kD) alternative variant c [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004143 EMBL-EBI Biotin/lipoate A/B protein ligase domain 9.4E-12
Pfam IPR004143 EMBL-EBI Biotin/lipoate A/B protein ligase domain 9.4E-12
Pfam IPR004143 EMBL-EBI Biotin/lipoate A/B protein ligase domain 9.4E-12
Pfam IPR004143 EMBL-EBI Biotin/lipoate A/B protein ligase domain 9.4E-12
Pfam IPR004143 EMBL-EBI Biotin/lipoate A/B protein ligase domain 9.4E-12
Pfam IPR007148 EMBL-EBI Dip2/Utp12 1.2E-104
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 6.3E-5
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.1E-10
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.5E-6
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 3.8E-4
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.14
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.9E-8
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.4E-10
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.5E-9
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.7E-6
Sequence
Target Sequence 
>C. ELEGANS:192207_AT
ttcctttgtccacgtgggatggcaatgttcaacttttctttttctatctcaaaaaagagc
agaatcgcaaaacatcttccaattctccagcacattttctgtgtggcacttgttgaagca
gcacggaatctatctggatatccagagtttccactgcatatcaaatggccaaatgatctg
tattgtgaacgatctcataaagttggtggaatgcttcttcagtgcagtactagggatgat
tcattccgagtcagtatcggatgtggaatgaatgtttcgaacgataaaccgacgatgtgc
ttgaatgatatgctgcctaaagaagccgaaacacggattacaaaagaacaattgattgcg
gaaacaataaacaaattcacttattacatgaaggactatgaggataatggcccggaaacc
ttcaaaaagaagtatcacgagtactggctgcattcacaacaagaagttctactgtcagac
ttcaatgaacgagtaacgattcgaggaatcgacgatgacggatacctgcaagtacgcagc
aagtccaaccc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCCTTTGTCCACGTGGGATGGCAA 175 691 6112 Antisense
GCAATGTTCAACTTTTCTTTTTCTA 547 319 6133 Antisense
CCAATTCTCCAGCACATTTTCTGTG 376 277 6190 Antisense
GATATCCAGAGTTTCCACTGCATAT 18 427 6248 Antisense
GGAATGCTTCTTCAGTGCAGTACTA 214 533 6319 Antisense
GGATGATTCATTCCGAGTCAGTATC 368 513 6345 Antisense
TGTGCTTGAATGATATGCTGCCTAA 59 557 6407 Antisense
GAGGATAATGGCCCGGAAACCTTCA 611 377 6511 Antisense
TATCACGAGTACTGGCTGCATTCAC 527 659 6544 Antisense
GAATCGACGATGACGGATACCTGCA 640 329 6617 Antisense
TGCAAGTACGCAGCAAGTCCAACCC 656 573 6638 Antisense
Sequence Source Affymetrix Proprietary Database