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[192202_s_at] Gamma-like tubulin

Description F58A4.8 /REP_DB=WormBase Gene ID /WP=CE00224 /GEN=tbg-1 /TR=SW:P34475 /GB=CAA80164.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Gamma-like tubulin
Cluster 6grp_highest_10_10 > 98      6grp_high1_10_10 > 87      4grp_highest_8_8 > 49      4grp_high_8_8 > 41      6grp_highest_nonorm > 96      6grp_total > 96      4grp_total > 54      6grp_highest_sub > Cu      6grp_cgc > 68      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 279
1 2 3 4 range
A 768.8
(3)
P 		693.7
(0.1)
P 		693.8
(0.1)
P 		614.2
(-3)
P 		155
B 815.6
(3)
P 		729.8
(-0.8)
P 		702.6
(-2)
P 		679.1
(-3)
P 		137
C 893.3
(3)
P 		724
(-0.7)
P 		659.7
(-2.1)
P 		619.9
(-3)
P 		273
avr 825.9
(3) 		715.8
(-0.5) 		685.4
(-1.5) 		637.7
(-3) 		188

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 898
1 2 3 4 5 6 range
A 695.2
(-1.4)
P 		659.7
(-1.7)
P 		462.6
(-3)
P 		884.2
(-0.2)
P 		896.3
(-0.1)
P 		1360.6
(3)
P 		898
B 813.4
(0.4)
P 		481.3
(-3)
P 		614.7
(-1.6)
P 		762.6
(-0.1)
P 		900.4
(1.3)
P 		1059.5
(3)
P 		578
C 731.5
(-0.7)
P 		519.9
(-3)
P 		705
(-0.9)
P 		978.3
(2.1)
P 		972
(2)
P 		1061.5
(3)
P 		542
avr 746.7
(-1.1) 		553.6
(-3) 		594.1
(-2.6) 		875
(0.2) 		922.9
(0.7) 		1160.5
(3) 		607
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 206
1 2 3 4 range
A 279.9
(3)
P 		150.6
(-1.1)
P 		181.4
(-0.1)
P 		89.9
(-3)
P 		190
B 260.1
(3)
P 		180.2
(-1.2)
P 		162.8
(-2.1)
P 		144.7
(-3)
P 		115
C 295.7
(3)
P 		208.2
(0.3)
P 		229.9
(0.9)
P 		104.5
(-3)
P 		191
avr 278.6
(3) 		179.7
(-0.6) 		191.4
(-0.2) 		113
(-3) 		166

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 294
1 2 3 4 5 6 range
A 128.2
(-2.3)
P 		234.6
(0.5)
P 		100.7
(-3)
P 		224.3
(0.2)
P 		270.5
(1.4)
P 		331.3
(3)
P 		231
B 283
(0.3)
P 		160.2
(-2.7)
P 		149.3
(-3)
P 		202
(-1.7)
P 		365.8
(2.3)
P 		395.1
(3)
P 		246
C 271.1
(0.4)
P 		158.6
(-3)
P 		179.4
(-2.4)
P 		196.7
(-1.8)
P 		314.5
(1.8)
P 		355.4
(3)
P 		197
avr 227.4
(-0.7) 		184.5
(-1.9) 		143.1
(-3) 		207.7
(-1.2) 		316.9
(1.8) 		360.6
(3) 		218
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192202_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7453
Sequence Type Exemplar sequence
Representative Public ID F58A4.8 NCBI
Target Description F58A4.8 /REP_DB=WormBase Gene ID /WP=CE00224 /GEN=tbg-1 /TR=SW:P34475 /GB=CAA80164.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Gamma-like tubulin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066730(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066730 NCBI Caenorhabditis elegans TuBulin Gamma family member (tbg-1) (tbg-1) mRNA, complete cds. 11/11 None
SNAP00000012198 ENSEMBL cdna:SNAP chromosome:CEL140:III:9626573:9629050:-1 11/11 None
GENEFINDER00000012209 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:9626573:9629050:-1 11/11 None
F58A4.8.1 ENSEMBL cdna:known chromosome:CEL140:III:9626440:9629050:-1 gene:F58A4.8 11/11 None
F58A4.8.2 ENSEMBL cdna:known chromosome:CEL140:III:9626451:9629052:-1 gene:F58A4.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9626568-9629046(-) 100.0 100.0
Public Domain and Genome References
Gene Title Gamma-like tubulin
Gene Symbol tbg-1
Ensembl F58A4.8
Entrez Gene 176361 Entrez gene
SwissProt P34475 EMBL-EBI
WormBase CE00224 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7018 microtubule-based movement inferred from electronic annotation QuickGO AmiGO
46785 microtubule polymerization inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5874 microtubule inferred from electronic annotation QuickGO AmiGO
45298 tubulin inferred from electronic annotation QuickGO AmiGO
5813 centrosome inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3924 GTPase activity inferred from electronic annotation QuickGO AmiGO
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
5200 structural constituent of cytoskeleton inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA80164 Hypothetical protein F58A4.8 [Caenorhabditis elegans] ref|NP_499131.1| TuBulin Gamma family member (tbg-1) [Caenorhabditis elegans] gb|AAG24513.1| gamma-tubulin [Caenorhabditis elegans] sp|P34475|TBG_CAEEL Tubulin gamma chain (Gamma tubulin) 0.0
blast CAE65165 Hypothetical protein CBG10035 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003008 EMBL-EBI Tubulin/FtsZ, GTPase 1.1E-82
Pfam IPR008280 EMBL-EBI Tubulin/FtsZ, C-terminal 4.1E-21
Sequence
Target Sequence 
>C. ELEGANS:192202_S_AT
tcatgagcacttccactgctccatatcgattcaactctgcaatgtgcccatcaattcggt
atttggatttggctccgtttccaccaatgcactttattcaatccgctatctctcctgttg
tcgatccaaatgagaactttacccgaaaaacttcagtcgctgacgtcactcgcttcttgt
taaaaccaacttcaatgatggtttcaactgcttctcgggttcggccaaatgactgcatgc
tatcagcttacatgttccttcaaggacaaattgaggcacatacaattatgactgctgagc
aaaacgttgatttcgcaatcagaaggcctccattctacatgcttaaaccacttcgaatga
tgcatgctccactctctccatatgttcggccacaatacaaagtatctggactcttgctca
ataacagcacaagcgtggctccgttattcgaatcgcttctctccaaatacgaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCATGAGCACTTCCACTGCTCCATA 23 623 722 Antisense
GCTCCATATCGATTCAACTCTGCAA 258 299 739 Antisense
CTCTGCAATGTGCCCATCAATTCGG 489 231 756 Antisense
AATGCACTTTATTCAATCCGCTATC 664 165 807 Antisense
TCTCTCCTGTTGTCGATCCAAATGA 684 611 830 Antisense
CGTCACTCGCTTCTTGTTAAAACCA 488 239 885 Antisense
TCTCGGGTTCGGCCAAATGACTGCA 495 609 934 Antisense
TGCTATCAGCTTACATGTTCCTTCA 703 579 959 Antisense
ATCAGAAGGCCTCCATTCTACATGC 249 27 1039 Antisense
AGCGTGGCTCCGTTATTCGAATCGC 218 75 1153 Antisense
GAATCGCTTCTCTCCAAATACGACA 187 329 1171 Antisense
Sequence Source Affymetrix Proprietary Database