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[192193_at] EF-hand calcium binding protein

Description F43C9.2 /REP_DB=WormBase Gene ID /WP=CE04565 /TR=Q20358 /GB=AAA81468.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=EF-hand calcium binding protein
Cluster 6grp_highest_10_10 > 20      6grp_high1_10_10 > 14      4grp_highest_8_8 > 07      4grp_high_8_8 > 21      6grp_highest_nonorm > 31      6grp_total > 04      4grp_total > 18      6grp_highest_sub > De      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 559
1 2 3 4 range
A 784.3
(-1)
P 		598
(-3)
P 		689.1
(-2)
P 		1157.2
(3)
P 		559
B 641.8
(-3)
P 		860.5
(3)
P 		744.5
(-0.2)
P 		803.6
(1.4)
P 		219
C 854
(-1.3)
P 		965.5
(3)
P 		812.2
(-3)
P 		811.4
(-3)
P 		154
avr 760
(-2.6) 		808
(-1) 		748.6
(-3) 		924.1
(3) 		176

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1347
1 2 3 4 5 6 range
A 1240
(3)
P 		1076.3
(1.6)
P 		760.3
(-1.1)
P 		1196.6
(2.6)
P 		700.9
(-1.7)
P 		545.7
(-3)
P 		694
B 1663
(3)
P 		1193
(0.9)
P 		582.7
(-1.8)
P 		535.1
(-2)
P 		588.1
(-1.8)
P 		316
(-3)
P 		1347
C 1402.3
(3)
P 		1212
(1.7)
P 		974.5
(0.1)
P 		1071
(0.8)
P 		910.2
(-0.3)
P 		503.4
(-3)
P 		899
avr 1435.1
(3) 		1160.4
(1.3) 		772.5
(-1.1) 		934.2
(-0.1) 		733.1
(-1.3) 		455
(-3) 		980
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 156
1 2 3 4 range
A 285.6
(3)
P 		129.8
(-3)
P 		180.1
(-1.1)
P 		169.4
(-1.5)
P 		156
B 204.7
(1.9)
P 		212.5
(3)
P 		172.5
(-2.8)
P 		171.3
(-3)
P 		41
C 282.7
(3)
P 		277.6
(2.8)
P 		283.1
(3)
P 		136.8
(-3)
P 		146
avr 257.7
(3) 		206.6
(-0.1) 		211.9
(0.2) 		159.2
(-3) 		99

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 461
1 2 3 4 5 6 range
A 228.6
(-0.7)
P 		382.8
(3)
P 		165.6
(-2.2)
P 		303.5
(1.1)
P 		211.5
(-1.1)
P 		132.9
(-3)
P 		250
B 578.6
(3)
P 		397.1
(0.6)
P 		141.5
(-2.7)
P 		141.7
(-2.7)
P 		238.9
(-1.4)
P 		117.8
(-3)
P 		461
C 519.8
(3)
P 		369.8
(0.4)
P 		248
(-1.6)
P 		215.4
(-2.2)
P 		294.5
(-0.8)
P 		168.5
(-3)
P 		351
avr 442.3
(3) 		383.2
(1.8) 		185
(-2.1) 		220.2
(-1.4) 		248.3
(-0.8) 		139.7
(-3) 		303
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192193_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17004
Sequence Type Exemplar sequence
Representative Public ID F43C9.2 NCBI
Target Description F43C9.2 /REP_DB=WormBase Gene ID /WP=CE04565 /TR=Q20358 /GB=AAA81468.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=EF-hand calcium binding protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076417
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076417 NCBI Caenorhabditis elegans F43C9.2 (F43C9.2) mRNA, complete cds. None
F43C9.2 ENSEMBL cdna:known chromosome:CEL140:X:4786086:4790269:1 gene:F43C9.2 None
SNAP00000037148 ENSEMBL cdna:SNAP chromosome:CEL140:X:4788492:4790118:1 None
GENEFINDER00000037156 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:4786206:4790118:1 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4788492-4790500(+) 100.0 100.0
Public Domain and Genome References
Gene Title EF-hand calcium binding protein
Ensembl F43C9.2
Entrez Gene 180753 Entrez gene
SwissProt Q20358 EMBL-EBI
WormBase CE29319 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA81468 Hypothetical protein F43C9.2 [Caenorhabditis elegans] ref|NP_508818.1| F43C9.2 [Caenorhabditis elegans] 2.0E-85
blast CAE68649 Hypothetical protein CBG14543 [Caenorhabditis briggsae] 4.0E-82
blast AAA81468 Hypothetical protein F43C9.2 [Caenorhabditis elegans] ref|NP_508818.1| F43C9.2 [Caenorhabditis elegans] 4.0E-72
blast CAE68649 Hypothetical protein CBG14543 [Caenorhabditis briggsae] 6.0E-69
blast XP_742310 calmodulin, putative [Plasmodium chabaudi] ref|XP_675328.1| calmodulin, putative [Plasmodium berghei] emb|CAH78331.1| calmodulin, putative [Plasmodium chabaudi] emb|CAH99328.1| calmodulin, putative [Plasmodium berghei] gb|EAA19232.1| calmodulin [Plasmodium yoelii yoelii] 9.0E-20
blast XP_742310 calmodulin, putative [Plasmodium chabaudi] ref|XP_675328.1| calmodulin, putative [Plasmodium berghei] emb|CAH78331.1| calmodulin, putative [Plasmodium chabaudi] emb|CAH99328.1| calmodulin, putative [Plasmodium berghei] gb|EAA19232.1| calmodulin [Plasmodium yoelii yoelii] 4.0E-19
Protein Domains
Database ID Description E-Value
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 4.4E-6
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 4.8E-8
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 2.4E-7
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 5.0E-6
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.5E-6
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 4.8E-8
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 2.4E-7
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 5.0E-6
Sequence
Target Sequence 
>C. ELEGANS:192193_AT
aatcgctgctcttctgagaactatcaatgtggagccaactcgcgtggagttggacttcat
ttttggagaaatggatacagataaaaccggaaaaatcagcaaagaagagttcgtcaacta
catgaagtctcctccaattcacagaaccactctccgcgaactcgaagtccagttccgcaa
gtttgattcagatggtgacggagctatcactgaagacgagatggccgaaattctacgaag
aaccgctgatctaggggatcgcgcagctatcagcgacatgttcaaggcgactgatttgaa
tggggatgctcaccaccccgtgttgttcattcctttccagaatcttcttttttttaatgt
accacttttaccactgaataaactagtcgcactttcaacactatacaaagtttcacatgc
ttcatccaccctcgcccaacaacaacaacaacaaaatgcccacccaaagcatcagctgga
tgaccacgttttcactcaaagcccaccctccttcactatttttttttccaattttccaca
tccgtctttgttata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATCGCTGCTCTTCTGAGAACTATC 39 169 123 Antisense
TCAATGTGGAGCCAACTCGCGTGGA 561 631 146 Antisense
AACTACATGAAGTCTCCTCCAATTC 681 139 238 Antisense
GAAGTCCAGTTCCGCAAGTTTGATT 546 335 286 Antisense
GAACCGCTGATCTAGGGGATCGCGC 219 347 362 Antisense
GCAGCTATCAGCGACATGTTCAAGG 268 313 385 Antisense
TTGAATGGGGATGCTCACCACCCCG 411 699 418 Antisense
ACAAAGTTTCACATGCTTCATCCAC 44 113 527 Antisense
GCCCACCCAAAGCATCAGCTGGATG 578 285 580 Antisense
AGCTGGATGACCACGTTTTCACTCA 607 79 596 Antisense
ATTTTCCACATCCGTCTTTGTTATA 573 13 653 Antisense
Sequence Source Affymetrix Proprietary Database