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[192192_at] ubiquitin-conjugating enzyme

Description C06E2.3 /REP_DB=WormBase Gene ID /WP=CE03946 /TR=SW:P52484 /GB=AAA80415.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=ubiquitin-conjugating enzyme
Cluster 6grp_total > 93      4grp_total > 13      6grp_cgc > 99      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 48
1 2 3 4 range
A 30.6
(-0.5)
P 		16.4
(-3)
A 		23
(-1.9)
A 		51.1
(3)
A 		35
B 42.1
(1.8)
P 		15.2
(-3)
A 		26.9
(-0.9)
A 		49
(3)
A 		34
C 48.4
(0.6)
P 		38
(-1.1)
A 		26.2
(-3)
A 		63.1
(3)
P 		37
avr 40.4
(0.3) 		23.2
(-3) 		25.4
(-2.6) 		54.4
(3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 65
1 2 3 4 5 6 range
A 16.2
(-1.9)
A 		26.2
(-0.9)
A 		32.9
(-0.3)
M 		28.5
(-0.7)
A 		69
(3)
A 		3.7
(-3)
A 		65
B 9.5
(-2.4)
A 		32.8
(2.2)
A 		37.1
(3)
A 		6.7
(-3)
A 		27.9
(1.2)
A 		28.6
(1.3)
P 		30
C 33.7
(1.4)
P 		39.4
(2.6)
M 		13.7
(-3)
A 		22.4
(-1.1)
A 		41.1
(3)
P 		40.3
(2.8)
P 		27
avr 19.8
(-2.9) 		32.8
(0) 		27.9
(-1.1) 		19.2
(-3) 		46
(3) 		24.2
(-1.9) 		27
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 11.1
(3)
P 		3.6
(-3)
A 		6
(-1.1)
A 		7.5
(0.1)
A 		8
B 13.4
(3)
P 		3.8
(-3)
A 		6.2
(-1.5)
A 		10.4
(1.1)
A 		10
C 16
(3)
P 		10.9
(-1.4)
A 		9.1
(-3)
A 		10.6
(-1.7)
P 		7
avr 13.5
(3) 		6.1
(-3) 		7.1
(-2.2) 		9.5
(-0.2) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 3
(-2.4)
A 		9.3
(-0.5)
A 		7.2
(-1.1)
M 		7.2
(-1.1)
A 		20.8
(3)
A 		0.9
(-3)
A 		20
B 3.3
(-2.1)
A 		10.9
(2.7)
A 		9
(1.5)
A 		1.8
(-3)
A 		11.4
(3)
A 		10.7
(2.6)
P 		10
C 12.5
(2.4)
P 		12
(2.1)
M 		3.5
(-3)
A 		4.5
(-2.4)
A 		13.3
(2.9)
P 		13.5
(3)
P 		10
avr 6.3
(-2) 		10.7
(0.5) 		6.6
(-1.8) 		4.5
(-3) 		15.2
(3) 		8.4
(-0.8) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192192_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16488
Sequence Type Exemplar sequence
Representative Public ID C06E2.3 NCBI
Target Description C06E2.3 /REP_DB=WormBase Gene ID /WP=CE03946 /TR=SW:P52484 /GB=AAA80415.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=ubiquitin-conjugating enzyme
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077101(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077101 NCBI Caenorhabditis elegans UBiquitin Conjugating enzyme family member (ubc-21) (ubc-21) mRNA, complete cds. 11/11 None
GENEFINDER00000017261 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:8869604:8872352:1 10/11 None
C06E2.3 ENSEMBL cdna:known chromosome:CEL140:X:8869562:8871806:1 gene:C06E2.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000017258 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:8869560-8871805(+) 100.0 100.0
Public Domain and Genome References
Gene Title ubiquitin-conjugating enzyme
Gene Symbol ubc-21
Ensembl C06E2.3
Entrez Gene 182321 Entrez gene
SwissProt P52484 EMBL-EBI
WormBase CE03946 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6464 protein modification inferred from electronic annotation QuickGO AmiGO
6512 ubiquitin cycle inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4840 ubiquitin conjugating enzyme activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA80415 Ubiquitin conjugating enzyme protein 21 [Caenorhabditis elegans] ref|NP_509502.1| UBiquitin Conjugating enzyme family member (ubc-21) [Caenorhabditis elegans] sp|P52484|UBC21_CAEEL Probable ubiquitin-conjugating enzyme E2 21 (Ubiquitin-protein ligase 21) (Ubiquitin carrier protein 21) 1.0E-148
blast AAA80415 Ubiquitin conjugating enzyme protein 21 [Caenorhabditis elegans] ref|NP_509502.1| UBiquitin Conjugating enzyme family member (ubc-21) [Caenorhabditis elegans] sp|P52484|UBC21_CAEEL Probable ubiquitin-conjugating enzyme E2 21 (Ubiquitin-protein ligase 21) (Ubiquitin carrier protein 21) 1.0E-108
blast CAE69374 Hypothetical protein CBG15473 [Caenorhabditis briggsae] 1.0E-79
blast CAE69374 Hypothetical protein CBG15473 [Caenorhabditis briggsae] 6.0E-77
blast CAE69517 Hypothetical protein CBG15726 [Caenorhabditis briggsae] 2.0E-55
blast CAE69517 Hypothetical protein CBG15726 [Caenorhabditis briggsae] 3.0E-55
Protein Domains
Database ID Description E-Value
Pfam IPR000608 EMBL-EBI Ubiquitin-conjugating enzymes 2.0E-62
Pfam IPR000608 EMBL-EBI Ubiquitin-conjugating enzymes 2.0E-62
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000017261 1 215-237
Sequence
Target Sequence 
>C. ELEGANS:192192_AT
tcaagggtccagagggcacaccttacgccggaggtacttttgaaataaaagtcgatattc
ctgaacattatccgtttgagccgccaaaggttaccgaaattatatttcacattcgcgcat
ttgagtatatacaggcaaaatttgttacacgaatctggcatccaaacatcagctcacaaa
ctggaaccatttgtttggacatactcaaggataaatggactgcaagtcttacacttcgga
cagttcttctatcacttcaggccatgctctgttctcctgaaccatcggaccctcaagatg
cagtggttgccaaacagtttatcaataattatccaatgtttacggcgaccgctgtctact
ggacttcttacttcgcaaactctaagaaagatgttgaacctgatttcaatagaaaagtcg
ggcgtctcatagagatgggaatcagagaggtaaaattttcaaatgacaaattttctagga
agcttaagtttgtaatttttagactgaagcaattgtctacctcagctgcaacaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCAAGGGTCCAGAGGGCACACCTTA 585 627 182 Antisense
CTGAACATTATCCGTTTGAGCCGCC 664 237 242 Antisense
TTGAGCCGCCAAAGGTTACCGAAAT 87 677 257 Antisense
ATTATATTTCACATTCGCGCATTTG 495 13 280 Antisense
GGCATCCAAACATCAGCTCACAAAC 300 537 338 Antisense
GGACTGCAAGTCTTACACTTCGGAC 413 525 398 Antisense
TTCGGACAGTTCTTCTATCACTTCA 406 695 416 Antisense
GGACCCTCAAGATGCAGTGGTTGCC 671 523 468 Antisense
ATTATCCAATGTTTACGGCGACCGC 671 13 509 Antisense
TCTACTGGACTTCTTACTTCGCAAA 523 613 536 Antisense
CAATTGTCTACCTCAGCTGCAACAA 371 179 691 Antisense
Sequence Source Affymetrix Proprietary Database