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[192191_s_at]

Description C06E7.1 /REP_DB=WormBase Gene ID /WP=CE03957 /CHR=4 /FEA=Sanger Annotation /DEF=S-adenosylmethionine synthetase (ST.LOUIS) SW:P50305 protein_id:AAA82280.1
Cluster 6grp_highest_10_10 > 95      6grp_high1_10_10 > 69      4grp_highest_8_8 > 18      4grp_high_8_8 > 62      6grp_highest_nonorm > 83      6grp_total > 49      4grp_total > 57      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1388
1 2 3 4 range
A 714
(-0.8)
P 		1203.2
(1.5)
P 		1512.9
(3)
P 		260.5
(-3)
P 		1252
B 661.6
(-1.4)
P 		1072.4
(0.9)
P 		1452.4
(3)
P 		366.9
(-3)
P 		1086
C 761.7
(-0.8)
P 		1151.1
(0.9)
P 		1647.5
(3)
P 		259.9
(-3)
P 		1388
avr 712.4
(-1) 		1142.2
(1.1) 		1537.6
(3) 		295.8
(-3) 		1242

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 440
1 2 3 4 5 6 range
A 378.1
(-0.7)
P 		495.8
(3)
P 		304.4
(-3)
P 		441.4
(1.3)
P 		481
(2.5)
P 		496.2
(3)
P 		192
B 511.3
(0.2)
P 		516
(0.3)
P 		336.2
(-3)
P 		363.7
(-2.5)
P 		535
(0.6)
P 		664.9
(3)
P 		329
C 602.9
(1)
P 		500.1
(-0.4)
P 		312.6
(-3)
P 		354.4
(-2.4)
P 		527.4
(-0)
P 		744.4
(3)
P 		432
avr 497.4
(0.4) 		504
(0.5) 		317.7
(-3) 		386.5
(-1.7) 		514.5
(0.7) 		635.2
(3) 		318
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 536
1 2 3 4 range
A 260
(0.7)
P 		261.2
(0.7)
P 		395.5
(3)
P 		38.1
(-3)
P 		357
B 211
(0.1)
P 		264.9
(1.3)
P 		336.6
(3)
P 		78.2
(-3)
P 		258
C 252.1
(-0.6)
P 		330.9
(0.2)
P 		574.2
(3)
P 		43.8
(-3)
P 		530
avr 241
(-0.1) 		285.7
(0.6) 		435.4
(3) 		53.4
(-3) 		382

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 183
1 2 3 4 5 6 range
A 69.7
(-2.8)
P 		176.3
(3)
P 		66.3
(-3)
P 		111.9
(-0.5)
P 		145.2
(1.3)
P 		120.8
(-0)
P 		110
B 177.9
(0.5)
P 		171.8
(0.3)
P 		81.6
(-3)
P 		96.3
(-2.5)
P 		217.4
(1.9)
P 		247.9
(3)
P 		166
C 223.5
(2.1)
P 		152.6
(-0.3)
P 		79.6
(-2.7)
P 		71.3
(-3)
P 		170.7
(0.4)
P 		249.2
(3)
P 		178
avr 157
(0.7) 		166.9
(1.2) 		75.8
(-3) 		93.2
(-2.2) 		177.8
(1.7) 		206
(3) 		130
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192191_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11234
Sequence Type Exemplar sequence
Representative Public ID C06E7.1 NCBI
Target Description C06E7.1 /REP_DB=WormBase Gene ID /WP=CE03957 /CHR=4 /FEA=Sanger Annotation /DEF=S-adenosylmethionine synthetase (ST.LOUIS) SW:P50305 protein_id:AAA82280.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_182283(11), NM_070500(11), NM_068471(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_182283 NCBI Caenorhabditis elegans Y105C5B.12b (Y105C5B.12) mRNA, complete cds. 11/11 A 
NM_070500 NCBI Caenorhabditis elegans Y105C5B.12a (Y105C5B.12) mRNA, complete cds. 11/11 A 
NM_068471 NCBI Caenorhabditis elegans C06E7.1a (C06E7.1) mRNA, complete cds. 11/11 None
SNAP00000018396 ENSEMBL cdna:SNAP chromosome:CEL140:IV:15942497:15943751:-1 11/11 None
GENEFINDER00000018426 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:15942497:15944392:-1 11/11 None
SNAP00000039817 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5848673:5850582:1 11/11 None
GENEFINDER00000039828 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5848673:5850582:1 11/11 None
C06E7.1a ENSEMBL cdna:known chromosome:CEL140:IV:5848640:5850822:1 gene:C06E7.1 11/11 None
Y105C5B.12b ENSEMBL cdna:known chromosome:CEL140:IV:15942223:15944416:-1 gene:Y105C5B.12 11/11 A 
Y105C5B.12a ENSEMBL cdna:known chromosome:CEL140:IV:15942497:15944153:-1 gene:Y105C5B.12 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177355 4 1 NM_068471 192191_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5848678-5850588(+) 100.0 100.0
chrIV:15942502-15944398(-) 97.7 97.94
Public Domain and Genome References
Ensembl C06E7.1
Y105C5B.12
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB54357 Hypothetical protein Y105C5B.12b [Caenorhabditis elegans] ref|NP_872083.1| Y105C5B.12b [Caenorhabditis elegans] 0.0
blast AAA82280 Hypothetical protein C06E7.1a [Caenorhabditis elegans] ref|NP_500872.1| C06E7.1a [Caenorhabditis elegans] sp|P50305|METK_CAEEL Probable S-adenosylmethionine synthetase C06E7.1 (Methionine adenosyltransferase) (AdoMet synthetase) 0.0
blast CAD56249 Hypothetical protein Y105C5B.12a [Caenorhabditis elegans] ref|NP_502901.2| Y105C5B.12a [Caenorhabditis elegans] 0.0
blast AAA82279 Hypothetical protein C06E7.3a [Caenorhabditis elegans] ref|NP_500871.1| C06E7.3a [Caenorhabditis elegans] sp|P50306|METL_CAEEL Probable S-adenosylmethionine synthetase C06E7.3 (Methionine adenosyltransferase) (AdoMet synthetase) 0.0
blast CAE72641 Hypothetical protein CBG19843 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 1.7E-66
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 4.4E-99
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 9.3E-95
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 9.9E-27
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 1.5E-7
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 4.4E-99
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 1.6E-92
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 4.3E-9
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 4.4E-99
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 1.6E-92
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 1.3E-25
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 4.4E-99
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 1.6E-92
Sequence
Target Sequence 
>C. ELEGANS:192191_S_AT
tcaaatcaggactctgtcgccgttgtcttgttcaagtatcttatgcaatcggagtagcca
aaccactttcagtgatggtgttctctttcgggacatctgcgttgaacgaaggagaactat
tgaaaattgtcaatgacaactttgatttgcgacctggaatgattattaaggatctcgatt
tgaagaaaccaatttatgaaccaaccgctgagaacggacactttggacacaatgaattcc
catggga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCAAATCAGGACTCTGTCGCCGTTG 498 627 893 Antisense
GTTCAAGTATCTTATGCAATCGGAG 651 445 922 Antisense
GCAATCGGAGTAGCCAAACCACTTT 581 321 937 Antisense
AGCCAAACCACTTTCAGTGATGGTG 118 85 948 Antisense
TTTCAGTGATGGTGTTCTCTTTCGG 695 669 959 Antisense
ACATCTGCGTTGAACGAAGGAGAAC 580 103 985 Antisense
ATGACAACTTTGATTTGCGACCTGG 145 43 1025 Antisense
GATTTGCGACCTGGAATGATTATTA 274 427 1036 Antisense
GAAACCAATTTATGAACCAACCGCT 193 313 1077 Antisense
ACCAACCGCTGAGAACGGACACTTT 426 83 1092 Antisense
TTGGACACAATGAATTCCCATGGGA 457 709 1115 Antisense
Sequence Source Affymetrix Proprietary Database