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[192185_at]

Description ZK836.3 /REP_DB=WormBase Gene ID /WP=CE15419 /GEN=vab-8 /TR=Q23630 /GB=CAB01589.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=kinensin-like protein
Cluster 6grp_high1_10_10 > 23      4grp_high_8_8 > 24      6grp_total > 35      4grp_total > 39      6grp_cgc > 44      6grp_high1_sub > 02      6grp_cgc_sub > 02      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 190
1 2 3 4 range
A 228.2
(3)
P 		99.6
(-1.2)
P 		46.3
(-3)
A 		138.8
(0.1)
P 		182
B 236.4
(3)
P 		100.1
(-3)
P 		124
(-1.9)
P 		126.2
(-1.9)
A 		136
C 206.8
(3)
P 		111.8
(-2.1)
P 		94.7
(-3)
P 		120.2
(-1.6)
P 		112
avr 223.8
(3) 		103.8
(-2.3) 		88.3
(-3) 		128.4
(-1.2) 		136

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 164
1 2 3 4 5 6 range
A 76.7
(-3)
A 		189.6
(3)
P 		79.2
(-2.9)
P 		133.4
(0)
P 		79.3
(-2.9)
A 		114.4
(-1)
P 		113
B 109.3
(2.5)
P 		116.9
(3)
P 		92.7
(1.4)
P 		25.5
(-3)
A 		62.5
(-0.6)
M 		64.8
(-0.4)
P 		91
C 145.5
(2.2)
P 		121.6
(0.7)
P 		158.4
(3)
P 		64.1
(-3)
A 		77.5
(-2.1)
P 		81.7
(-1.9)
P 		94
avr 110.5
(0.2) 		142.7
(3) 		110.1
(0.2) 		74.3
(-2.9) 		73.1
(-3) 		87
(-1.8) 		70
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 71
1 2 3 4 range
A 83.1
(3)
P 		21.6
(-2.2)
P 		12.1
(-3)
A 		20.3
(-2.3)
P 		71
B 75.4
(3)
P 		24.7
(-3)
P 		28.7
(-2.5)
P 		26.9
(-2.7)
A 		51
C 68.4
(3)
P 		32.2
(-1.5)
P 		33
(-1.4)
P 		20.3
(-3)
P 		48
avr 75.6
(3) 		26.2
(-2.6) 		24.6
(-2.8) 		22.5
(-3) 		53

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 61
1 2 3 4 5 6 range
A 14.1
(-3)
A 		67.4
(3)
P 		17.3
(-2.6)
P 		33.8
(-0.8)
P 		23.9
(-1.9)
A 		27.9
(-1.4)
P 		53
B 38
(2.8)
P 		38.9
(3)
P 		22.5
(-0.1)
P 		6.7
(-3)
A 		25.4
(0.5)
M 		24.2
(0.3)
P 		32
C 53.9
(3)
P 		37.1
(0.5)
P 		40.3
(1)
P 		12.9
(-3)
A 		25.1
(-1.2)
P 		27.4
(-0.9)
P 		41
avr 35.3
(0.5) 		47.8
(3) 		26.7
(-1.2) 		17.8
(-3) 		24.8
(-1.6) 		26.5
(-1.3) 		30
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192185_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14443
Sequence Type Exemplar sequence
Representative Public ID ZK836.3 NCBI
Target Description ZK836.3 /REP_DB=WormBase Gene ID /WP=CE15419 /GEN=vab-8 /TR=Q23630 /GB=CAB01589.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=kinensin-like protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073662(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073662 NCBI Caenorhabditis elegans Variable ABnormal morphology family member (vab-8) (vab-8) mRNA, complete cds. 9/11 A 
GENEFINDER00000005778 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:12215924:12222623:1 9/11 None
K12F2.2a ENSEMBL cdna:known chromosome:CEL140:V:12215915:12228508:1 gene:K12F2.2 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000005777 1/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
179675 2 1 NM_073662 192185_at (9)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:12215923-12219439(+) 100.0 100.0
Public Domain and Genome References
Ensembl K12F2.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAD36504 Hypothetical protein K12F2.2a [Caenorhabditis elegans] emb|CAB01577.2| Hypothetical protein K12F2.2a [Caenorhabditis elegans] ref|NP_506063.2| Variable ABnormal morphology family member (vab-8) [Caenorhabditis elegans] gb|AAF17300.1| VAB-8L [Caenorhabditis elegans] 0.0
blast CAB54275 Hypothetical protein K12F2.2b [Caenorhabditis elegans] ref|NP_506064.1| Variable ABnormal morphology family member (vab-8) [Caenorhabditis elegans] gb|AAF17301.1| VAB-8S [Caenorhabditis elegans] 0.0
blast CAE75420 Hypothetical protein CBG23411 [Caenorhabditis briggsae] 0.0
blast T28033 hypothetical protein ZK836.3 - Caenorhabditis elegans 0.0
blast CAE75418 Hypothetical protein CBG23408 [Caenorhabditis briggsae] 1.0E-174
Sequence
Target Sequence 
>C. ELEGANS:192185_AT
cgtcgtcgactttgtttcttggatatgggaattggtgagagaaattcaacaaatggagga
atgacaatgccagcattagggagtattcttttggcaatggttcaaagaaataaacatatt
ccatcaagggactcgtcagtgtgtcaactcatccgatgtgcattgagtacgtcaagattc
acgacgttcgttttttcatttggtgccaagagtgacgataacgagaacattgcacatttg
gcttgtaaaatcgcaaggactcgagcaaagagtatggttggacatggaaggaagtcatca
gggacaatgtcaactggaacaatggaatcaaattcttcatcatgtggaacaactacaatc
acaccgggaggcactcctagaacacaacgaagatttgaattagaatccggatcagagcta
agtgcagctgaaactgtgatattcttgggtccgaattcctcaagaacagcatcaccagct
tccacaacaagtgagtttgaaaaaatcttcgggatgtgggtaaagaagattaatcgactg
agatctgatttacgagccgcccgcattttttttacgctcgttcgcatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGTCGTCGACTTTGTTTCTTGGATA 342 243 721 Antisense
AAGGGACTCGTCAGTGTGTCAACTC 174 159 846 Antisense
GTGTCAACTCATCCGATGTGCATTG 50 487 861 Antisense
GTTCGTTTTTTCATTTGGTGCCAAG 31 443 906 Antisense
ACAACTACAATCACACCGGGAGGCA 418 111 1069 Antisense
CGGGAGGCACTCCTAGAACACAACG 422 243 1085 Antisense
GAATCCGGATCAGAGCTAAGTGCAG 275 329 1123 Antisense
GATATTCTTGGGTCCGAATTCCTCA 230 427 1158 Antisense
TCAAGAACAGCATCACCAGCTTCCA 556 631 1180 Antisense
ACTGAGATCTGATTTACGAGCCGCC 547 97 1257 Antisense
CATTTTTTTTACGCTCGTTCGCATC 528 215 1284 Antisense
Sequence Source Affymetrix Proprietary Database