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[192181_at] vacuolar processing enzyme like

Description T28H10.3 /REP_DB=WormBase Gene ID /WP=CE14367 /TR=Q17945 /GB=CAA99935.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=vacuolar processing enzyme like
Cluster 6grp_highest_10_10 > 59      6grp_high1_10_10 > 94      4grp_highest_8_8 > 23      4grp_high_8_8 > 06      6grp_highest_nonorm > 49      6grp_total > 85      4grp_total > 03      6grp_highest_sub > In      6grp_high1_sub > In      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6404
1 2 3 4 range
A 2230.7
(-2.9)
P 		2201.4
(-2.9)
P 		2092.4
(-3)
P 		7829.7
(3)
P 		5737
B 2705.2
(-2.3)
P 		2097.4
(-2.9)
P 		1993.4
(-3)
P 		7847.4
(3)
P 		5854
C 2553.8
(-2.3)
P 		1755.8
(-3)
P 		2194
(-2.6)
P 		8160
(3)
P 		6404
avr 2496.6
(-2.5) 		2018.2
(-3) 		2093.3
(-2.9) 		7945.7
(3) 		5928

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5442
1 2 3 4 5 6 range
A 1332.2
(-3)
P 		2571.8
(-1.6)
P 		3651.8
(-0.4)
P 		6774.4
(3)
P 		4823.9
(0.8)
P 		6683.3
(2.9)
P 		5442
B 1676
(-3)
P 		2056.8
(-2.5)
P 		4141.7
(0.5)
P 		5272.4
(2.1)
P 		4709.7
(1.3)
P 		5884
(3)
P 		4208
C 1812.8
(-3)
P 		2346.1
(-2.3)
P 		4052.1
(-0.2)
P 		5059
(1)
P 		5769
(1.9)
P 		6651.7
(3)
P 		4839
avr 1607
(-3) 		2324.9
(-2.1) 		3948.5
(-0.1) 		5701.9
(2.1) 		5100.9
(1.4) 		6406.3
(3) 		4799
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1210
1 2 3 4 range
A 812.2
(0)
P 		477.9
(-3)
P 		547
(-2.4)
P 		1146.1
(3)
P 		668
B 862.7
(-1)
P 		518
(-2.7)
P 		461.9
(-3)
P 		1672.3
(3)
P 		1210
C 845.4
(-0.7)
P 		504.8
(-3)
P 		764.7
(-1.2)
P 		1375.9
(3)
P 		871
avr 840.1
(-0.7) 		500.2
(-3) 		591.2
(-2.4) 		1398.1
(3) 		898

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1981
1 2 3 4 5 6 range
A 245.6
(-3)
P 		914.7
(-0.3)
P 		795.3
(-0.8)
P 		1718.1
(3)
P 		1455.6
(1.9)
P 		1627.2
(2.6)
P 		1473
B 583.2
(-3)
P 		684.6
(-2.6)
P 		1005.7
(-1.4)
P 		1396.5
(0)
P 		1913.5
(2)
P 		2194.1
(3)
P 		1611
C 671.9
(-3)
P 		715.9
(-2.8)
P 		1031.3
(-1.6)
P 		1017.4
(-1.7)
P 		1866.8
(1.6)
P 		2226.7
(3)
P 		1555
avr 500.2
(-3) 		771.7
(-1.9) 		944.1
(-1.2) 		1377.3
(0.5) 		1745.3
(1.9) 		2016
(3) 		1516
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192181_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13154
Sequence Type Exemplar sequence
Representative Public ID T28H10.3 NCBI
Target Description T28H10.3 /REP_DB=WormBase Gene ID /WP=CE14367 /TR=Q17945 /GB=CAA99935.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=vacuolar processing enzyme like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073736(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073736 NCBI Caenorhabditis elegans T28H10.3 (T28H10.3) mRNA, complete cds. 11/11 None
SNAP00000034296 ENSEMBL cdna:SNAP chromosome:CEL140:V:12516263:12517459:1 11/11 None
GENEFINDER00000034302 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:12516263:12517459:1 11/11 None
T28H10.3 ENSEMBL cdna:known chromosome:CEL140:V:12515609:12517521:1 gene:T28H10.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:12515679-12517459(+) 100.0 100.0
Public Domain and Genome References
Gene Title vacuolar processing enzyme like
Ensembl T28H10.3
Entrez Gene 179714 Entrez gene
SwissProt Q17945 EMBL-EBI
WormBase CE14367 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:257130_AT vacuolar processing enzyme, putative / asparaginyl endopeptidase, putative at
CANINE:1585709_X_AT similar to Legumain precursor (Asparaginyl endopeptidase) (Protease, cysteine 1) cfa
CANINE:1585708_S_AT similar to Legumain precursor (Asparaginyl endopeptidase) (Protease, cysteine 1) cfa
CANINE:1584313_AT similar to Legumain precursor (Asparaginyl endopeptidase) (Protease, cysteine 1) cfa
CANINE_2:CFA.11935.1.A1_AT similar to Legumain precursor (Asparaginyl endopeptidase) (Protease, cysteine 1) cfa
CANINE_2:CFA.20896.1.S1_S_AT similar to Legumain precursor (Asparaginyl endopeptidase) (Protease, cysteine 1) cfa
CANINE_2:CFA.8631.1.A1_AT similar to Legumain precursor (Asparaginyl endopeptidase) (Protease, cysteine 1) cfa
CANINE_2:CFAAFFX.17218.1.S1_AT similar to Legumain precursor (Asparaginyl endopeptidase) (Protease, cysteine 1) cfa
CANINE_2:CFAAFFX.17220.1.S1_S_AT similar to Legumain precursor (Asparaginyl endopeptidase) (Protease, cysteine 1) cfa
CHICKEN:GGA.6319.2.S1_A_AT similar to legumain gga
HG-U95AV2:317_AT legumain hs
HC-G110:317_AT legumain hs
HG-U133_PLUS_2:201212_AT legumain hs
HG-U133A:201212_AT legumain hs
HG-FOCUS:201212_AT legumain hs
HG-U133A_2:201212_AT legumain hs
HU35KSUBD:RC_R27007_S_AT legumain hs
HUGENEFL:D55696_AT legumain hs
U133_X3P:G1890049_3P_AT legumain hs
HG-U95C:51728_AT legumain hs
MU11KSUBB:MSA.5619.0_S_AT legumain mm
MG-U74AV2:93261_AT legumain mm
MOE430A:1448883_AT legumain mm
MOUSE430A_2:1448883_AT legumain mm
MOUSE430_2:1448883_AT legumain mm
RAE230A:1368430_AT legumain rn
RAT230_2:1368430_AT legumain rn
RAE230B:1380609_AT Legumain rn
RAT230_2:1380609_AT Legumain rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
1509 legumain activity inferred from electronic annotation QuickGO AmiGO
4197 cysteine-type endopeptidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB01126 Hypothetical protein T28H10.3 [Caenorhabditis elegans] emb|CAA99935.1| Hypothetical protein T28H10.3 [Caenorhabditis elegans] ref|NP_506137.1| T28H10.3 [Caenorhabditis elegans] 0.0
blast CAE75506 Hypothetical protein CBG23516 [Caenorhabditis briggsae] 0.0
blast CAB01126 Hypothetical protein T28H10.3 [Caenorhabditis elegans] emb|CAA99935.1| Hypothetical protein T28H10.3 [Caenorhabditis elegans] ref|NP_506137.1| T28H10.3 [Caenorhabditis elegans] 1.0E-171
blast CAE75506 Hypothetical protein CBG23516 [Caenorhabditis briggsae] 1.0E-158
blast AAF21773 hemoglobinase-type cysteine proteinase [Caenorhabditis elegans] 1.0E-107
Protein Domains
Database ID Description E-Value
Pfam IPR001096 EMBL-EBI Peptidase C13, legumain 1.0E-126
Pfam IPR001096 EMBL-EBI Peptidase C13, legumain 1.4E-51
Sequence
Target Sequence 
>C. ELEGANS:192181_AT
tcttgaattccaatacgagctagttaaaaaagagacaaacctttcccatgtgatgcagtt
tggtgataaggatattgcgaaagaggcagtagccttgttccaaggagataaggaagatag
agagtatgtcgaagattttggattgtctgcttcaaaatctgtcaactggccagcaagaga
tattgagctcaatcacttgatttcgcaacacagaaagtcgaatgatttgttatcgtcgaa
caagttagagtacaaaatcaatagaatcaaggagacacgaagagcaatcaagagaaatgt
acatatgattgttcagaagttctttgatggagagtctgaagatctgatcagccgagtact
aacacaaactcgtccagttcttgatctccgttgccatcacattgctgttcatcttttcaa
aaagtactgtatcaatttcaatgaatatgagtacgcgatgaagtatgtgaaagtcatcaa
caacatgtgcatatatcgtcgaatcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTTGAATTCCAATACGAGCTAGTT 121 619 807 Antisense
GAGACAAACCTTTCCCATGTGATGC 84 393 838 Antisense
AACCTTTCCCATGTGATGCAGTTTG 308 137 844 Antisense
AGAGGCAGTAGCCTTGTTCCAAGGA 344 67 888 Antisense
GTCGAAGATTTTGGATTGTCTGCTT 273 475 934 Antisense
AAATCTGTCAACTGGCCAGCAAGAG 101 119 961 Antisense
GTCGAATGATTTGTTATCGTCGAAC 119 475 1023 Antisense
AGAAGTTCTTTGATGGAGAGTCTGA 440 73 1121 Antisense
GATCTGATCAGCCGAGTACTAACAC 371 419 1147 Antisense
TTGCCATCACATTGCTGTTCATCTT 238 701 1197 Antisense
AACATGTGCATATATCGTCGAATCG 551 137 1288 Antisense
Sequence Source Affymetrix Proprietary Database