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[192180_s_at] Ubiquitin conjugating enzyme

Description F58A4.10 /REP_DB=WormBase Gene ID /WP=CE00216 /TR=SW:P34477 /GB=CAA80166.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Ubiquitin conjugating enzyme
Cluster 6grp_highest_10_10 > 59      6grp_high1_10_10 > 94      4grp_highest_8_8 > 13      4grp_high_8_8 > 30      6grp_highest_nonorm > 86      6grp_total > 85      4grp_total > 24      6grp_highest_sub > In      6grp_cgc > 77      6grp_high1_sub > In      6grp_cgc_sub > 04_05_06      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 380
1 2 3 4 range
A 736.9
(-3)
P 		894.2
(-0.4)
P 		866.9
(-0.9)
P 		1106.1
(3)
P 		369
B 736.3
(-3)
P 		866.7
(-0.5)
P 		929.8
(0.8)
P 		1044.5
(3)
P 		308
C 726.4
(-3)
P 		909.1
(0.4)
P 		935.2
(0.9)
P 		1046.2
(3)
P 		320
avr 733.2
(-3) 		890
(-0.2) 		910.6
(0.2) 		1065.6
(3) 		332

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 512
1 2 3 4 5 6 range
A 716.1
(-3)
P 		772.7
(-2.1)
P 		876.5
(-0.6)
P 		1110.5
(3)
P 		944.6
(0.5)
P 		1039.5
(1.9)
P 		394
B 732.1
(-3)
P 		871.7
(-1.3)
P 		857.6
(-1.5)
P 		916.8
(-0.8)
P 		1227.8
(3)
P 		1173.3
(2.3)
P 		496
C 1020.2
(1)
P 		759.3
(-3)
P 		841.1
(-1.7)
P 		1109
(2.4)
P 		959.5
(0.1)
P 		1146.8
(3)
P 		388
avr 822.8
(-2.6) 		801.2
(-3) 		858.4
(-1.9) 		1045.4
(1.6) 		1044
(1.6) 		1119.9
(3) 		319
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 164
1 2 3 4 range
A 268.3
(3)
P 		194.1
(-1.2)
P 		226.6
(0.6)
P 		161.9
(-3)
P 		106
B 234.8
(3)
P 		214.1
(-3)
P 		215.5
(-2.6)
P 		222.6
(-0.5)
P 		21
C 240.4
(-0.4)
P 		261.4
(0.4)
P 		326
(3)
P 		176.4
(-3)
P 		150
avr 247.8
(2.3) 		223.2
(0.1) 		256
(3) 		187
(-3) 		69

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 367
1 2 3 4 5 6 range
A 132
(-3)
P 		274.8
(2.6)
P 		190.9
(-0.7)
P 		281.6
(2.9)
P 		285
(3)
P 		253.1
(1.7)
P 		153
B 254.7
(-2)
P 		290.1
(-1.3)
P 		208.2
(-3)
P 		242.8
(-2.3)
P 		498.8
(3)
P 		437.5
(1.7)
P 		291
C 378.1
(2.8)
P 		231.7
(-2.4)
P 		214.1
(-3)
P 		223
(-2.7)
P 		310.5
(0.4)
P 		383.9
(3)
P 		170
avr 254.9
(-1.1) 		265.5
(-0.7) 		204.4
(-3) 		249.1
(-1.3) 		364.8
(3) 		358.2
(2.8) 		160
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192180_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7656
Sequence Type Exemplar sequence
Representative Public ID F58A4.10 NCBI
Target Description F58A4.10 /REP_DB=WormBase Gene ID /WP=CE00216 /TR=SW:P34477 /GB=CAA80166.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Ubiquitin conjugating enzyme
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066732(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066732 NCBI Caenorhabditis elegans UBiquitin Conjugating enzyme family member (ubc-7) (ubc-7) mRNA, complete cds. 11/11 None
SNAP00000012200 ENSEMBL cdna:SNAP chromosome:CEL140:III:9630371:9631059:-1 11/11 None
GENEFINDER00000012205 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:9630371:9631059:-1 11/11 None
F58A4.10.1 ENSEMBL cdna:known chromosome:CEL140:III:9630002:9631065:-1 gene:F58A4.10 11/11 A 
F58A4.10.2 ENSEMBL cdna:known chromosome:CEL140:III:9630086:9631063:-1 gene:F58A4.10 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9630366-9631055(-) 100.0 100.0
Public Domain and Genome References
Gene Title Ubiquitin conjugating enzyme
Gene Symbol ubc-7
Ensembl F58A4.10
Entrez Gene 176363 Entrez gene
SwissProt P34477 EMBL-EBI
WormBase CE00216 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:17852_G_AT ubiquitin-conjugating enzyme 14 (UBC14) at
ATGENOME1:17851_AT ubiquitin-conjugating enzyme 14 (UBC14) at
ATH1-121501:251802_AT ubiquitin-conjugating enzyme 14 (UBC14) at
DROSOPHILA_2:1633683_AT dm
DROSGENOME1:149858_AT dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6464 protein modification inferred from electronic annotation QuickGO AmiGO
6512 ubiquitin cycle inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4840 ubiquitin conjugating enzyme activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA80166 Hypothetical protein F58A4.10 [Caenorhabditis elegans] ref|NP_499133.1| UBiquitin Conjugating enzyme family member (ubc-7) [Caenorhabditis elegans] sp|P34477|UBC7_CAEEL Probable ubiquitin-conjugating enzyme E2 7 (Ubiquitin-protein ligase 7) (Ubiquitin carrier protein 7) pdb|1PZV|A Chain A, Crystal Structures Of Two Ubc (E2) Enzymes Of The Ubiquitin- Conjugating System In Caenorhabditis Elegans 1.0E-93
blast CAE65167 Hypothetical protein CBG10037 [Caenorhabditis briggsae] 5.0E-93
Protein Domains
Database ID Description E-Value
Pfam IPR000608 EMBL-EBI Ubiquitin-conjugating enzymes 1.4E-82
Sequence
Target Sequence 
>C. ELEGANS:192180_S_AT
agacatgcgcagagttccagtcgacggattctcggcaggtctcgtcgatgacaatgacat
ctacaaatgggaagtcctggtgatcggaccaccagatactctctacgagggaggtttctt
caaggctattctcgactttccaagggattacccacaaaaaccaccaaagatgaagttcat
ttcggaaatttggcatccaaacattgacaaggaaggtaacgtgtgcatctcgattcttca
cgatcccggagatgataagtggggttacgaacgtccggaagagcgttggctgcctgtgca
tactgtcgagacgatccttctttctgtcatctcaatgctcacggatcctaactttgaatc
tcctgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGACATGCGCAGAGTTCCAGTCGAC 524 71 51 Antisense
TCGGCAGGTCTCGTCGATGACAATG 127 607 82 Antisense
GGGAAGTCCTGGTGATCGGACCACC 97 287 119 Antisense
GGAGGTTTCTTCAAGGCTATTCTCG 255 517 160 Antisense
GCTATTCTCGACTTTCCAAGGGATT 91 305 175 Antisense
TAACGTGTGCATCTCGATTCTTCAC 450 639 267 Antisense
GATTCTTCACGATCCCGGAGATGAT 323 431 282 Antisense
AACGTCCGGAAGAGCGTTGGCTGCC 184 145 320 Antisense
GCCTGTGCATACTGTCGAGACGATC 7 283 342 Antisense
TTCTTTCTGTCATCTCAATGCTCAC 531 689 368 Antisense
CGGATCCTAACTTTGAATCTCCTGC 409 243 392 Antisense
Sequence Source Affymetrix Proprietary Database