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[192167_at] Eukaryotic protein kinase domain

Description R06C7.8 /REP_DB=WormBase Gene ID /WP=CE06251 /TR=Q21776 /GB=CAA95845.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Eukaryotic protein kinase domain
Cluster 6grp_highest_10_10 > 97      6grp_high1_10_10 > 89      4grp_high_8_8 > 60      6grp_highest_nonorm > 93      6grp_total > 89      4grp_total > 60      6grp_highest_sub > Cu      6grp_cgc > 69      6grp_high1_sub > Cu      6grp_cgc_sub > Da      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 456
1 2 3 4 range
A 443.7
(1.4)
P 		359.7
(0.2)
P 		567
(3)
P 		116.1
(-3)
A 		451
B 292.4
(0.2)
P 		435.9
(2.7)
P 		454.2
(3)
P 		111.5
(-3)
P 		343
C 393.1
(1.7)
P 		368.9
(1.3)
P 		465.1
(3)
P 		121.9
(-3)
A 		343
avr 376.4
(1.1) 		388.2
(1.3) 		495.4
(3) 		116.5
(-3) 		379

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 653
1 2 3 4 5 6 range
A 402.2
(-1)
P 		341.9
(-1.6)
P 		197.9
(-3)
P 		287.8
(-2.1)
P 		591.3
(0.8)
P 		818.9
(3)
P 		621
B 500.6
(-0.4)
P 		290.7
(-2.4)
P 		228
(-3)
P 		397.3
(-1.4)
P 		658.5
(1.1)
P 		850.7
(3)
P 		623
C 437
(-0.6)
P 		363.7
(-1.5)
P 		244.9
(-3)
P 		387.7
(-1.2)
P 		577.1
(1.1)
P 		725.8
(3)
P 		481
avr 446.6
(-0.7) 		332.1
(-1.9) 		223.6
(-3) 		357.6
(-1.6) 		609
(1) 		798.5
(3) 		575
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 145
1 2 3 4 range
A 161.6
(3)
P 		78.1
(-0.5)
P 		148.2
(2.4)
P 		17
(-3)
A 		145
B 93.3
(2)
P 		107.7
(3)
P 		105.2
(2.8)
P 		23.8
(-3)
P 		84
C 130.1
(1.6)
P 		106.1
(0.6)
P 		162.1
(3)
P 		20.6
(-3)
A 		142
avr 128.3
(2.5) 		97.3
(0.9) 		138.5
(3) 		20.5
(-3) 		118

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 274
1 2 3 4 5 6 range
A 74.1
(-1.8)
P 		121.6
(0)
P 		43.1
(-3)
P 		73
(-1.9)
P 		178.4
(2.2)
P 		199.4
(3)
P 		156
B 174.2
(-0.3)
P 		96.8
(-2.1)
P 		55.4
(-3)
P 		105.2
(-1.9)
P 		267.5
(1.9)
P 		317.2
(3)
P 		262
C 162
(0.3)
P 		111
(-1.4)
P 		62.3
(-3)
P 		78
(-2.5)
P 		186.7
(1.1)
P 		243
(3)
P 		181
avr 136.8
(-0.5) 		109.8
(-1.3) 		53.6
(-3) 		85.4
(-2) 		210.9
(1.7) 		253.2
(3) 		200
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192167_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2430
Sequence Type Exemplar sequence
Representative Public ID R06C7.8 NCBI
Target Description R06C7.8 /REP_DB=WormBase Gene ID /WP=CE06251 /TR=Q21776 /GB=CAA95845.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Eukaryotic protein kinase domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059650(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059650 NCBI Caenorhabditis elegans yeast BUB homolog family member (bub-1) (bub-1) mRNA, complete cds. 11/11 None
SNAP00000036118 ENSEMBL cdna:SNAP chromosome:CEL140:I:7260078:7263262:1 11/11 None
GENEFINDER00000036125 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:7260078:7263262:1 11/11 None
R06C7.8 ENSEMBL cdna:known chromosome:CEL140:I:7260072:7263356:1 gene:R06C7.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:7260008-7263193(+) 100.0 100.0
Public Domain and Genome References
Gene Title Eukaryotic protein kinase domain
Gene Symbol bub-1
Ensembl R06C7.8
Entrez Gene 172468 Entrez gene
SwissProt Q21776 EMBL-EBI
WormBase CE06251 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA95845 Hypothetical protein R06C7.8 [Caenorhabditis elegans] ref|NP_492051.1| yeast BUB homolog family member (bub-1) [Caenorhabditis elegans] 0.0
blast CAE65082 Hypothetical protein CBG09940 [Caenorhabditis briggsae] 0.0
blast CAE74229 Hypothetical protein CBG21913 [Caenorhabditis briggsae] 1.0E-141
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.1E-17
Sequence
Target Sequence 
>C. ELEGANS:192167_AT
gcacataacctgcttcttgattacccaaatggcccgagttgtgaaggaagtccatgcctc
taaaattattcatggagatatcaaaccggataattttatgatcaccagaaagatcgatga
taaatggggcaaagatgctctgatgagtaacgacagctttgtcatcaagattatcgactg
gggacgtgccattgacatgatgccactgaagaaccagcgtttcaagggaagagcaggaac
agaagcgtttgattgcccggaaatggttgacggtcgttcttggacttaccaagccgatta
cttcggattcgccgcaacaatggctgtcgttgttgcaggaaagtatgcacagttgactgg
agctagcgttggagactattcgttaaatgtcgacatcaagaggcgcaacattcttcgcga
tgcttgctacgatgtcattaacagattcctcaacatcccttcgtgtgattcacttccgga
ctggaatatcctgatcaagtctttctcggaaatctggaatgaaaagtttgaagcatcggg
atggcgacaggcggtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCACATAACCTGCTTCTTGATTACC 664 315 2376 Antisense
GATTACCCAAATGGCCCGAGTTGTG 112 421 2394 Antisense
GGGGACGTGCCATTGACATGATGCC 96 499 2555 Antisense
GAACAGAAGCGTTTGATTGCCCGGA 335 349 2612 Antisense
TTGACGGTCGTTCTTGGACTTACCA 9 683 2642 Antisense
TGGACTTACCAAGCCGATTACTTCG 412 527 2656 Antisense
GATTACTTCGGATTCGCCGCAACAA 622 429 2671 Antisense
TTCGCGATGCTTGCTACGATGTCAT 239 697 2789 Antisense
TCCCTTCGTGTGATTCACTTCCGGA 34 599 2831 Antisense
AATATCCTGATCAAGTCTTTCTCGG 685 173 2860 Antisense
AGCATCGGGATGGCGACAGGCGGTT 571 77 2907 Antisense
Sequence Source Affymetrix Proprietary Database