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[192158_at]

Description F56B6.2 /REP_DB=WormBase Gene ID /WP=CE04662 /TR=Q22998 /GB=AAB04563.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=helix-loop-helix phosphoprotein
Cluster 6grp_high1_10_10 > 25      6grp_total > 25      4grp_total > 39      6grp_cgc > 43      6grp_high1_sub > 01_02      6grp_cgc_sub > 02      6grp_total_sub > Decrease      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 408
1 2 3 4 range
A 509.6
(3)
P 		227.1
(-2.1)
A 		176.7
(-3)
A 		279.5
(-1.1)
A 		333
B 546.2
(3)
P 		210.4
(-1.9)
A 		137.8
(-3)
A 		222.7
(-1.8)
A 		408
C 470.3
(3)
P 		224.6
(-1.9)
A 		171
(-3)
A 		257.9
(-1.3)
A 		299
avr 508.7
(3) 		220.7
(-2) 		161.8
(-3) 		253.4
(-1.4) 		347

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 229
1 2 3 4 5 6 range
A 288.2
(3)
P 		262.3
(1.8)
P 		217.6
(-0.2)
P 		228
(0.3)
P 		176.5
(-2)
P 		154.6
(-3)
A 		134
B 319.4
(1.2)
P 		367.1
(3)
P 		261.8
(-0.9)
P 		203.7
(-3)
P 		255.7
(-1.1)
P 		215
(-2.6)
P 		163
C 265.5
(0.4)
A 		365.1
(3)
P 		165.9
(-2.3)
M 		173.3
(-2.1)
P 		138.2
(-3)
A 		210.3
(-1.1)
P 		227
avr 291
(1.3) 		331.5
(3) 		215.1
(-1.9) 		201.7
(-2.5) 		190.1
(-3) 		193.3
(-2.9) 		141
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 154
1 2 3 4 range
A 185.6
(3)
P 		49.3
(-2.7)
A 		46.2
(-2.8)
A 		40.9
(-3)
A 		145
B 174.2
(3)
P 		52
(-2.2)
A 		31.9
(-3)
A 		47.5
(-2.3)
A 		142
C 155.7
(3)
P 		64.6
(-1.9)
A 		59.6
(-2.1)
A 		43.5
(-3)
A 		112
avr 171.8
(3) 		55.3
(-2.5) 		45.9
(-2.9) 		44
(-3) 		128

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 87
1 2 3 4 5 6 range
A 53.1
(-1.3)
P 		93.3
(3)
P 		47.4
(-1.9)
P 		57.8
(-0.8)
P 		53.3
(-1.3)
P 		37.6
(-3)
A 		56
B 111.1
(2)
P 		122.2
(3)
P 		63.6
(-2.2)
P 		54
(-3)
P 		103.9
(1.4)
P 		80.2
(-0.7)
P 		68
C 98.4
(2)
A 		111.4
(3)
P 		42.2
(-2.4)
M 		34.8
(-3)
P 		44.7
(-2.2)
A 		70.4
(-0.2)
P 		77
avr 87.5
(0.9) 		109
(3) 		51.1
(-2.8) 		48.9
(-3) 		67.3
(-1.2) 		62.7
(-1.6) 		60
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192158_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18583
Sequence Type Exemplar sequence
Representative Public ID F56B6.2 NCBI
Target Description F56B6.2 /REP_DB=WormBase Gene ID /WP=CE04662 /TR=Q22998 /GB=AAB04563.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=helix-loop-helix phosphoprotein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076209(9), NM_001029543(9), NM_001029544(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076209 NCBI Caenorhabditis elegans Regulator of G protein Signaling family member (rgs-7) (rgs-7) mRNA, complete cds. 9/11 A 
NM_001029543 NCBI Caenorhabditis elegans Regulator of G protein Signaling family member (rgs-7) (rgs-7) mRNA, complete cds. 9/11 None
NM_001029544 NCBI Caenorhabditis elegans Regulator of G protein Signaling family member (rgs-7) (rgs-7) mRNA, complete cds. 9/11 None
F56B6.2a ENSEMBL cdna:known chromosome:CEL140:X:3542545:3557582:1 gene:F56B6.2 9/11 A 
F56B6.2b ENSEMBL cdna:known chromosome:CEL140:X:3545393:3557159:1 gene:F56B6.2 9/11 None
F56B6.2c ENSEMBL cdna:known chromosome:CEL140:X:3547434:3557147:1 gene:F56B6.2 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000023813 1/11 Cross Hyb Matching Probes None
SNAP00000023806 2/11 Cross Hyb Matching Probes None
SNAP00000023809 8/11 Cross Hyb Matching Probes None
GENEFINDER00000023816 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:3546487-3557148(+) 100.0 100.0
Public Domain and Genome References
Ensembl F56B6.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAS75452 RGS-7A [Caenorhabditis elegans] gb|AAL11462.1| Regulator of g protein signaling protein 7, isoform a [Caenorhabditis elegans] ref|NP_508610.3| Regulator of G protein Signaling family member (rgs-7) [Caenorhabditis elegans] sp|Q8WQC0|RGS7_CAEEL Regulator of G-protein signaling rgs-7 (Regulator of G-protein signaling c2) (Protein C2-RGS) 0.0
blast AAL65791 Regulator of g protein signaling protein 7, isoform b [Caenorhabditis elegans] ref|NP_001024714.1| Regulator of G protein Signaling family member (rgs-7) [Caenorhabditis elegans] dbj|BAB85494.1| C2-RGS protein [Caenorhabditis elegans] 0.0
blast CAE68445 Hypothetical protein CBG14233 [Caenorhabditis briggsae] 0.0
blast AAL11463 Regulator of g protein signaling protein 7, isoform c [Caenorhabditis elegans] ref|NP_001024715.1| Regulator of G protein Signaling family member (rgs-7) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000008 EMBL-EBI C2 domain 3.1E-6
Pfam IPR000342 EMBL-EBI Regulator of G protein 3.6E-40
Pfam IPR000008 EMBL-EBI C2 domain 3.1E-6
Pfam IPR000342 EMBL-EBI Regulator of G protein 3.6E-40
Pfam IPR000342 EMBL-EBI Regulator of G protein 3.6E-40
Sequence
Target Sequence 
>C. ELEGANS:192158_AT
gctggttcatcggtgatttccaacaatactggaggacaggtgcggattgctcttaaagtg
atcggaccaatctcgcgcacgctctcctatttgcggtcgaagatggatttggctctctcc
acgtcatcactttatccatctcgcgatgatgtccgacagtgggaaatatcattcgaatct
ctacttaacaacaaattcggttgcgctctttttcgtcagtttttgaaaaaggagttttcc
gatgagaacatggacttttggctcgagtgtgaggagtttaagaagatgaaggacggaaag
aagtcgaccacacaaaaagccatcgagatttacagtgaatttgtcgctgaacactcaccc
aaagaggtgaacttggatagtgatacccgggccgccacaaaagctgccgttgaggccgga
tgtaagccagacaccttcgcgttggctcaaagccgtgttgagcaacttatgtccaaagat
tcgtacagacgattcctgcgtgaccgtctattcctagacctcctcgaaagctacga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTGGTTCATCGGTGATTTCCAACA 271 295 1030 Antisense
AGGACAGGTGCGGATTGCTCTTAAA 286 55 1062 Antisense
TAAAGTGATCGGACCAATCTCGCGC 351 639 1083 Antisense
GCTCTCCTATTTGCGGTCGAAGATG 306 299 1110 Antisense
TCATCACTTTATCCATCTCGCGATG 236 621 1153 Antisense
TGAATTTGTCGCTGAACACTCACCC 568 575 1365 Antisense
CGCCACAAAAGCTGCCGTTGAGGCC 262 255 1422 Antisense
GGCCGGATGTAAGCCAGACACCTTC 77 541 1443 Antisense
CCTTCGCGTTGGCTCAAAGCCGTGT 555 201 1463 Antisense
CGTACAGACGATTCCTGCGTGACCG 611 245 1511 Antisense
TCCTAGACCTCCTCGAAAGCTACGA 659 583 1541 Antisense
Sequence Source Affymetrix Proprietary Database