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[192150_at] NADH-ubiquinone oxidoreductase

Description T26A5.3 /REP_DB=WormBase Gene ID /WP=CE00702 /TR=Q22800 /GB=AAC77506.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=NADH-ubiquinone oxidoreductase
Cluster 6grp_highest_10_10 > 69      6grp_high1_10_10 > 96      4grp_highest_8_8 > 11      4grp_high_8_8 > 47      6grp_highest_nonorm > 94      6grp_total > 86      4grp_total > 48      6grp_highest_sub > In      6grp_cgc > 68      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 210
1 2 3 4 range
A 160.4
(-3)
P 		256.6
(0.7)
P 		315.6
(3)
P 		243.6
(0.2)
P 		155
B 199.8
(-3)
P 		277.7
(1.7)
P 		299
(3)
P 		232
(-1.1)
P 		99
C 193.2
(-2.6)
P 		303.8
(0.9)
P 		370.1
(3)
P 		180.9
(-3)
P 		189
avr 184.5
(-3) 		279.4
(1) 		328.2
(3) 		218.8
(-1.6) 		144

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1035
1 2 3 4 5 6 range
A 192.7
(-2.9)
P 		187.6
(-3)
P 		337.2
(-1.2)
P 		454.8
(0.2)
P 		675.1
(2.8)
P 		687.7
(3)
P 		500
B 230.2
(-2.7)
P 		183.1
(-3)
P 		329
(-2.1)
P 		488.3
(-1.1)
P 		576.7
(-0.6)
P 		1164.6
(3)
P 		982
C 141.8
(-3)
P 		224.7
(-2.5)
P 		224.8
(-2.5)
P 		520.4
(-0.8)
P 		486.2
(-1)
P 		1176.9
(3)
P 		1035
avr 188.2
(-3) 		198.5
(-2.9) 		297
(-2.2) 		487.8
(-0.8) 		579.3
(-0.1) 		1009.7
(3) 		822
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 99
1 2 3 4 range
A 58.4
(-0.1)
P 		55.7
(-0.4)
P 		82.5
(3)
P 		35.7
(-3)
P 		47
B 63.7
(1.3)
P 		68.6
(2.8)
P 		69.3
(3)
P 		49.4
(-3)
P 		20
C 64
(-1)
P 		87.3
(0.5)
P 		129
(3)
P 		30.5
(-3)
P 		99
avr 62
(-0.4) 		70.5
(0.5) 		93.6
(3) 		38.5
(-3) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 399
1 2 3 4 5 6 range
A 35.5
(-3)
P 		66.7
(-1.9)
P 		73.4
(-1.6)
P 		115.3
(-0.2)
P 		203.7
(3)
P 		167.4
(1.7)
P 		168
B 80.1
(-2.7)
P 		60.9
(-3)
P 		79.9
(-2.7)
P 		129.3
(-1.9)
P 		234.3
(-0.2)
P 		434.3
(3)
P 		373
C 52.6
(-3)
P 		68.6
(-2.7)
P 		57.2
(-2.9)
P 		104.7
(-2.1)
P 		157.3
(-1.2)
P 		394
(3)
P 		341
avr 56.1
(-3) 		65.4
(-2.8) 		70.2
(-2.7) 		116.4
(-1.7) 		198.4
(0.1) 		331.9
(3) 		276
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192150_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6603
Sequence Type Exemplar sequence
Representative Public ID T26A5.3 NCBI
Target Description T26A5.3 /REP_DB=WormBase Gene ID /WP=CE00702 /TR=Q22800 /GB=AAC77506.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=NADH-ubiquinone oxidoreductase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066022(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066022 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-99) (tag-99) mRNA, complete cds. 11/11 None
SNAP00000033273 ENSEMBL cdna:SNAP chromosome:CEL140:III:6465161:6467080:1 11/11 None
GENEFINDER00000033289 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:6465161:6467080:1 11/11 None
T26A5.3 ENSEMBL cdna:known chromosome:CEL140:III:6465139:6467120:1 gene:T26A5.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6465159-6467079(+) 100.0 100.0
Public Domain and Genome References
Gene Title NADH-ubiquinone oxidoreductase
Gene Symbol tag-99
Ensembl T26A5.3
Entrez Gene 188912 Entrez gene
SwissProt Q22800 EMBL-EBI
WormBase CE00702 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1601264_X_AT similar to NADH-ubiquinone oxidoreductase 49 kDa subunit, mitochondrial precursor (Complex I-49KD) (CI-49KD) cfa
CANINE:1601263_S_AT similar to NADH-ubiquinone oxidoreductase 49 kDa subunit, mitochondrial precursor (Complex I-49KD) (CI-49KD) cfa
CANINE:1592387_X_AT similar to NADH-ubiquinone oxidoreductase 49 kDa subunit, mitochondrial precursor (Complex I-49KD) (CI-49KD) cfa
CANINE_2:CFA.4438.1.S1_AT similar to NADH-ubiquinone oxidoreductase 49 kDa subunit, mitochondrial precursor (Complex I-49KD) (CI-49KD) cfa
CANINE_2:CFA.4438.1.S1_S_AT similar to NADH-ubiquinone oxidoreductase 49 kDa subunit, mitochondrial precursor (Complex I-49KD) (CI-49KD) cfa
HG-U133_PLUS_2:201966_AT NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase) hs
HG-FOCUS:201966_AT NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase) hs
HG-U133A:201966_AT NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase) hs
HG-U133A_2:201966_AT NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase) hs
HG-U95AV2:32738_AT NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase) hs
HU35KSUBC:RC_AA045978_S_AT NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase) hs
HU35KSUBD:RC_AA218948_AT NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase) hs
U133_X3P:G4758785_3P_AT NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase) hs
HU35KSUBC:RC_W15219_AT NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase) hs
MU11KSUBA:AA415929_S_AT NADH dehydrogenase (ubiquinone) Fe-S protein 2 mm
MG-U74AV2:93972_AT NADH dehydrogenase (ubiquinone) Fe-S protein 2 mm
MOE430A:1451096_AT NADH dehydrogenase (ubiquinone) Fe-S protein 2 mm
MOUSE430A_2:1451096_AT NADH dehydrogenase (ubiquinone) Fe-S protein 2 mm
MOUSE430_2:1451096_AT NADH dehydrogenase (ubiquinone) Fe-S protein 2 mm
MU11KSUBA:AA727622_AT NADH dehydrogenase (ubiquinone) Fe-S protein 2 (Ndufs2), mRNA mm
MU19KSUBB:TC33448_S_AT NADH dehydrogenase (ubiquinone) Fe-S protein 2 (Ndufs2), mRNA mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5489 electron transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_498423 Temporarily Assigned Gene name family member (tag-99) [Caenorhabditis elegans] gb|AAC77506.1| Temporarily assigned gene name protein 99 [Caenorhabditis elegans] 0.0
blast CAE72504 Hypothetical protein CBG19683 [Caenorhabditis briggsae] 0.0
blast CAB01886 Hypothetical protein K09A9.5 [Caenorhabditis elegans] ref|NP_510569.1| General Anaesthetic Sensitivity abnormal family member (gas-1) [Caenorhabditis elegans] sp|Q93873|NUCM_CAEEL Probable NADH-ubiquinone oxidoreductase 49 kDa subunit, mitochondrial precursor (Complex I-49KD) (CI-49KD) 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001135 EMBL-EBI NADH-ubiquinone oxidoreductase, chain 49kDa 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:192150_AT
ccgcatccgatgctcttaattggggattcagtggtgtgatggttcgtggaagtggaatta
agcaagatgttcgaaaaaccgaaccatatgatgcctatgccgatatggaattcgatgtac
caatcggaacaaagggagattgctatgatcgttatttgtgtcgcgtagaagaaatgagac
aatcgttgaacattgttcatcaatgcctcaacaaaatgccaactggagaaattaaatccg
acgatcacaaagtggtgccaccgaaacgagctgaaatgaaagagaacatggaatcgctta
ttcatcactttaaatttttcactgaaggattccaagtaccgcctggagctacatatgtac
cgattgaggctccaaagggagaatttggagtgtacctcgtggccgatggaacgggaaaac
cgtatcgatgctttatccgtgctcctggttttgcccatcttgccgcaattcacgacgtat
gctacatgtctctaatcgctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCGCATCCGATGCTCTTAATTGGGG 696 261 881 Antisense
ACCGAACCATATGATGCCTATGCCG 333 89 958 Antisense
TGATCGTTATTTGTGTCGCGTAGAA 683 563 1026 Antisense
GTGGTGCCACCGAAACGAGCTGAAA 254 489 1132 Antisense
CAAGTACCGCCTGGAGCTACATATG 278 183 1213 Antisense
ACATATGTACCGATTGAGGCTCCAA 168 103 1231 Antisense
GAATTTGGAGTGTACCTCGTGGCCG 501 325 1261 Antisense
GTACCTCGTGGCCGATGGAACGGGA 190 451 1272 Antisense
AAACCGTATCGATGCTTTATCCGTG 396 123 1297 Antisense
TCTTGCCGCAATTCACGACGTATGC 90 619 1338 Antisense
GTATGCTACATGTCTCTAATCGCTG 2 455 1357 Antisense
Sequence Source Affymetrix Proprietary Database