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[192149_s_at]

Description F10C1.2B /REP_DB=WormBase Gene ID /WP=CE02619 /TR=Q19289 /GB=AAA93407.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Intermediate filament protein
Cluster 6grp_highest_10_10 > 21      6grp_high1_10_10 > 04      4grp_highest_8_8 > 32      4grp_high_8_8 > 50      6grp_highest_nonorm > 15      6grp_total > 14      4grp_total > 61      6grp_highest_sub > De      6grp_cgc > 31      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6202
1 2 3 4 range
A 6066.2
(3)
P 		5465.1
(2.3)
P 		4265
(1.1)
P 		521.6
(-3)
P 		5545
B 6667.3
(3)
P 		4989.1
(1.4)
P 		4732.5
(1.1)
P 		537.3
(-3)
P 		6130
C 6097.8
(3)
P 		5285.6
(2.1)
P 		4709.1
(1.5)
P 		464.9
(-3)
P 		5633
avr 6277.1
(3) 		5246.6
(1.9) 		4568.9
(1.2) 		507.9
(-3) 		5769

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7449
1 2 3 4 5 6 range
A 9154.8
(3)
P 		6943.3
(1.1)
P 		4782.7
(-0.9)
P 		4395
(-1.2)
P 		4157.1
(-1.4)
P 		2346.4
(-3)
P 		6808
B 8413
(3)
P 		6745.5
(1.4)
P 		6588.6
(1.3)
P 		4881.2
(-0.3)
P 		3309.8
(-1.8)
P 		2079.2
(-3)
P 		6334
C 7119.9
(3)
P 		6542.5
(2.4)
P 		6824.6
(2.7)
P 		3964.5
(-0.5)
P 		3441.7
(-1.1)
P 		1705.7
(-3)
P 		5414
avr 8229.2
(3) 		6743.8
(1.6) 		6065.3
(0.9) 		4413.6
(-0.7) 		3636.2
(-1.5) 		2043.8
(-3) 		6185
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2132
1 2 3 4 range
A 2208.7
(3)
P 		1186.3
(0.1)
P 		1115
(-0.1)
P 		76.3
(-3)
P 		2132
B 2126.2
(3)
P 		1232.2
(0.3)
P 		1096.6
(-0.1)
P 		114.5
(-3)
P 		2012
C 2018.5
(3)
P 		1519.6
(1.5)
P 		1641.3
(1.8)
P 		78.4
(-3)
P 		1940
avr 2117.8
(3) 		1312.7
(0.6) 		1284.3
(0.5) 		89.7
(-3) 		2028

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2356
1 2 3 4 5 6 range
A 1687.6
(0.5)
P 		2469.4
(3)
P 		1041.6
(-1.5)
P 		1114.7
(-1.3)
P 		1254.4
(-0.8)
P 		571.3
(-3)
P 		1898
B 2927.3
(3)
P 		2245.3
(1.1)
P 		1599.9
(-0.7)
P 		1292.8
(-1.6)
P 		1344.7
(-1.4)
P 		775.3
(-3)
P 		2152
C 2639
(3)
P 		1996.3
(1.1)
P 		1737
(0.4)
P 		797.3
(-2.3)
P 		1113.7
(-1.4)
P 		571
(-3)
P 		2068
avr 2418
(3) 		2237
(2.4) 		1459.5
(-0.2) 		1068.3
(-1.6) 		1237.6
(-1) 		639.2
(-3) 		1779
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192149_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4062
Sequence Type Exemplar sequence
Representative Public ID F10C1.2B NCBI
Target Description F10C1.2B /REP_DB=WormBase Gene ID /WP=CE02619 /TR=Q19289 /GB=AAA93407.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Intermediate filament protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062735(11), NM_062736(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062735 NCBI Caenorhabditis elegans Intermediate Filament, B family member (ifb-1) (ifb-1) mRNA, complete cds. 11/11 None
NM_062736 NCBI Caenorhabditis elegans Intermediate Filament, B family member (ifb-1) (ifb-1) mRNA, complete cds. 11/11 None
SNAP00000018699 ENSEMBL cdna:SNAP chromosome:CEL140:II:5768926:5771656:1 11/11 None
GENEFINDER00000018703 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5768926:5771656:1 11/11 None
F10C1.2b.2 ENSEMBL cdna:known chromosome:CEL140:II:5765578:5772036:1 gene:F10C1.2 11/11 None
F10C1.2b.1 ENSEMBL cdna:known chromosome:CEL140:II:5765583:5772103:1 gene:F10C1.2 11/11 None
F10C1.2a.2 ENSEMBL cdna:known chromosome:CEL140:II:5768856:5771656:1 gene:F10C1.2 11/11 None
F10C1.2a.1 ENSEMBL cdna:known chromosome:CEL140:II:5768910:5771656:1 gene:F10C1.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5765603-5771653(+) 100.0 100.0
Public Domain and Genome References
Ensembl F10C1.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_495136 Intermediate Filament, B family member (ifb-1) [Caenorhabditis elegans] gb|AAA93407.1| Intermediate filament, b protein 1, isoform b [Caenorhabditis elegans] sp|Q19289|IFB1_CAEEL Intermediate filament protein ifb-1 (Intermediate filament protein B1) (IF-B1) (Cel IF B1) 0.0
blast NP_495137 Intermediate Filament, B family member (ifb-1) [Caenorhabditis elegans] gb|AAA93408.1| Intermediate filament, b protein 1, isoform a [Caenorhabditis elegans] 0.0
blast CAE59186 Hypothetical protein CBG02495 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001664 EMBL-EBI Intermediate filament protein 2.0E-15
Pfam IPR001664 EMBL-EBI Intermediate filament protein 1.9E-88
Pfam IPR001322 EMBL-EBI Intermediate filament, C-terminal 3.8E-76
Pfam IPR001664 EMBL-EBI Intermediate filament protein 2.0E-15
Pfam IPR001664 EMBL-EBI Intermediate filament protein 1.9E-88
Pfam IPR001322 EMBL-EBI Intermediate filament, C-terminal 3.8E-76
Sequence
Target Sequence 
>C. ELEGANS:192149_S_AT
acattggttgctgagcttcaagctcttttggataccaagcaaatgcttgacgccgaaatt
gccatctacagaaaaatgctcgagggagaagagaccagagtcggactcacccaaatggtt
gagcaagccgtcaagacgcattctctccaacaacaagaaaacactgattcgacaagatca
gttcgcggtgaggtatccaccaagaccaccttccaaagatccgccaagggaaatgtgaca
atttccgagtgtgatccaaatggaaagttcatcaagcttgagaactcgcacagaaacaag
gatgagaatgttggtgagcacaaaatcagaagaaagttggatggaagacgcgaaattgtc
tattccattccagccaatgttgttatcaagccaggaaagaacttgaccatctacgcccgt
gaccaaggaggcatcaacaacccaccagagtctcttgtcttcgatgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACATTGGTTGCTGAGCTTCAAGCTC 215 105 1210 Antisense
GCTTGACGCCGAAATTGCCATCTAC 399 307 1254 Antisense
GTCGGACTCACCCAAATGGTTGAGC 334 471 1309 Antisense
CAAGCCGTCAAGACGCATTCTCTCC 305 187 1333 Antisense
GATCAGTTCGCGGTGAGGTATCCAC 329 421 1385 Antisense
CACCTTCCAAAGATCCGCCAAGGGA 430 197 1416 Antisense
GTGACAATTTCCGAGTGTGATCCAA 108 483 1444 Antisense
GACGCGAAATTGTCTATTCCATTCC 471 381 1556 Antisense
TCTACGCCCGTGACCAAGGAGGCAT 525 611 1619 Antisense
ACCAAGGAGGCATCAACAACCCACC 250 85 1631 Antisense
CACCAGAGTCTCTTGTCTTCGATGG 602 201 1652 Antisense
Sequence Source Affymetrix Proprietary Database