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[192147_s_at] Heat shock 70Kd protein (HSP70)

Description F54C9.2 /REP_DB=WormBase Gene ID /WP=CE02252 /TR=Q20752 /GB=CAA90248.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=Heat shock 70Kd protein (HSP70)
Cluster 6grp_highest_10_10 > 02      6grp_high1_10_10 > 10      4grp_highest_8_8 > 11      4grp_high_8_8 > 47      6grp_highest_nonorm > 42      6grp_total > 50      4grp_total > 48      6grp_cgc > 60      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 403
1 2 3 4 range
A 447.3
(-3)
P
709.3
(3)
P
701
(2.8)
P
577.1
(-0)
P
262
B 509.7
(-2.1)
P
614.6
(0.6)
P
712
(3)
P
472.8
(-3)
P
239
C 515.7
(-3)
P
575.7
(-1.9)
P
850.5
(3)
P
647.8
(-0.6)
P
335
avr 490.9
(-3)
633.2
(0.2)
754.5
(3)
565.9
(-1.3)
264

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 426
1 2 3 4 5 6 range
A 966.7
(3)
P
768.2
(-0.9)
P
940.5
(2.5)
P
826.6
(0.2)
P
670.1
(-2.9)
P
664.3
(-3)
P
302
B 864.9
(1.9)
P
734.7
(-1.1)
P
915.4
(3)
P
821.6
(0.9)
P
791.8
(0.2)
P
649.5
(-3)
P
266
C 853.5
(1.2)
P
845.6
(1.1)
P
663.9
(-1.4)
P
979.2
(3)
P
553.1
(-3)
P
667.7
(-1.4)
P
426
avr 895
(3)
782.8
(0.1)
839.9
(1.6)
875.8
(2.5)
671.7
(-2.7)
660.5
(-3)
235
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 212
1 2 3 4 range
A 162.9
(1.8)
P
154
(1.2)
P
183.2
(3)
P
84.5
(-3)
P
99
B 162.5
(2.8)
P
151.8
(1.8)
P
165
(3)
P
100.8
(-3)
P
64
C 170.7
(-1)
P
165.5
(-1.2)
P
296.4
(3)
P
109.2
(-3)
P
187
avr 165.4
(0.5)
157.1
(0)
214.9
(3)
98.2
(-3)
117

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 160
1 2 3 4 5 6 range
A 178.2
(-2.1)
P
273.2
(3)
P
204.8
(-0.7)
P
209.7
(-0.4)
P
202.2
(-0.8)
P
161.7
(-3)
P
112
B 300.9
(1.8)
P
244.5
(-1.4)
P
222.3
(-2.7)
P
217.6
(-3)
P
321.7
(3)
P
242.2
(-1.6)
P
104
C 316.4
(3)
P
258
(0.6)
P
169
(-3)
P
196.9
(-1.9)
P
179
(-2.6)
P
223.5
(-0.8)
P
147
avr 265.2
(3)
258.6
(2.4)
198.7
(-3)
208.1
(-2.2)
234.3
(0.2)
209.1
(-2.1)
67
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192147_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3192
Sequence Type Exemplar sequence
Representative Public ID F54C9.2 NCBI
Target Description F54C9.2 /REP_DB=WormBase Gene ID /WP=CE02252 /TR=Q20752 /GB=CAA90248.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=Heat shock 70Kd protein (HSP70)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063407(11)
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_063407 NCBI Caenorhabditis elegans STCH (truncated HSP) family member (stc-1) (stc-1) mRNA, complete cds. 11/11 None
SNAP00000013419 ENSEMBL cdna:SNAP chromosome:CEL140:II:8563905:8565987:-1 11/11 None
GENEFINDER00000013449 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8563905:8566029:-1 11/11 None
F54C9.2 ENSEMBL cdna:known chromosome:CEL140:II:8563646:8566061:-1 gene:F54C9.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8563901-8566026(-) 100.0 100.0
Public Domain and Genome References
Gene Title Heat shock 70Kd protein (HSP70)
Gene Symbol stc-1
Ensembl F54C9.2
Entrez Gene 174368 Entrez gene
SwissProt Q20752 EMBL-EBI
WormBase CE32895 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.1481.1.S1_AT similar to Stress 70 protein chaperone microsome-associated 60 kDa protein precursor (Microsomal stress 70 protein ATPase core) cfa
CANINE_2:CFAAFFX.12859.1.S1_S_AT similar to Stress 70 protein chaperone microsome-associated 60 kDa protein precursor (Microsomal stress 70 protein ATPase core) cfa
CHICKEN:GGAAFFX.9978.1.S1_S_AT stress 70 protein chaperone, microsome-associated, 60kDa gga
HG-U95AV2:38677_AT stress 70 protein chaperone, microsome-associated, 60kDa hs
HG-U95AV2:38676_AT stress 70 protein chaperone, microsome-associated, 60kDa hs
HG-U133_PLUS_2:202558_S_AT stress 70 protein chaperone, microsome-associated, 60kDa hs
HG-U133A:202558_S_AT stress 70 protein chaperone, microsome-associated, 60kDa hs
HG-U133A_2:202558_S_AT stress 70 protein chaperone, microsome-associated, 60kDa hs
HU35KSUBC:RC_AA045519_AT stress 70 protein chaperone, microsome-associated, 60kDa hs
HUGENEFL:U04735_AT stress 70 protein chaperone, microsome-associated, 60kDa hs
U133_X3P:G5902125_3P_A_AT stress 70 protein chaperone, microsome-associated, 60kDa hs
U133_X3P:HS.288799.0.S2_3P_AT stress 70 protein chaperone, microsome-associated, 60kDa hs
HG-U133A_2:202557_AT stress 70 protein chaperone, microsome-associated, 60kDa hs
HG-FOCUS:202557_AT stress 70 protein chaperone, microsome-associated, 60kDa hs
HG-U133_PLUS_2:202557_AT stress 70 protein chaperone, microsome-associated, 60kDa hs
HG-U133A:202557_AT stress 70 protein chaperone, microsome-associated, 60kDa hs
MOE430B:1429502_AT stress 70 protein chaperone, microsome-associated, human homolog mm
MOUSE430_2:1429502_AT stress 70 protein chaperone, microsome-associated, human homolog mm
MG-U74BV2:113297_AT stress 70 protein chaperone, microsome-associated, human homolog mm
MG-U74BV2:108358_AT stress 70 protein chaperone, microsome-associated, human homolog mm
MG-U74CV2:168740_F_AT stress 70 protein chaperone, microsome-associated, human homolog mm
MG-U74CV2:167779_I_AT stress 70 protein chaperone, microsome-associated, human homolog mm
MG-U74CV2:166313_R_AT stress 70 protein chaperone, microsome-associated, human homolog mm
MG-U74CV2:170296_I_AT stress 70 protein chaperone, microsome-associated, human homolog mm
MOE430B:1453172_AT stress 70 protein chaperone, microsome-associated, human homolog mm
MOUSE430_2:1453172_AT stress 70 protein chaperone, microsome-associated, human homolog mm
MOE430B:1430026_AT stress 70 protein chaperone, microsome-associated, human homolog mm
MOUSE430_2:1430026_AT stress 70 protein chaperone, microsome-associated, human homolog mm
MOE430B:1433772_AT stress 70 protein chaperone, microsome-associated, human homolog mm
MOUSE430_2:1433772_AT stress 70 protein chaperone, microsome-associated, human homolog mm
MU19KSUBB:TC32026_AT Stress 70 protein chaperone, microsome-associated, human homolog, mRNA (cDNA clone MGC:113760 IMAGE:30646642) mm
MU19KSUBC:TC35753_AT Stress 70 protein chaperone, microsome-associated, human homolog, mRNA (cDNA clone MGC:113760 IMAGE:30646642) mm
RG-U34A:AF006617_AT stress 70 protein chaperone, microsome-associated, 60kD human homolog rn
RAE230A:1368844_AT stress 70 protein chaperone, microsome-associated, 60kD human homolog rn
RAT230_2:1368844_AT stress 70 protein chaperone, microsome-associated, 60kD human homolog rn
RAE230A:1372368_AT Stress 70 protein chaperone, microsome-associated, 60kD human homolog rn
RAT230_2:1372368_AT Stress 70 protein chaperone, microsome-associated, 60kD human homolog rn
RG-U34C:RC_AI137631_AT Stress 70 protein chaperone, microsome-associated, 60kD human homolog rn
RG-U34C:RC_AI230431_AT Stress 70 protein chaperone, microsome-associated, 60kD human homolog rn
Protein Similarities
Method ID Description E-Value
blast CAA90248 Hypothetical protein F54C9.2 [Caenorhabditis elegans] ref|NP_495808.2| STCH (truncated HSP) family member (stc-1) [Caenorhabditis elegans] 0.0
blast CAE57585 Hypothetical protein CBG00564 [Caenorhabditis briggsae] 0.0
blast XP_688977 PREDICTED: similar to Stress 70 protein chaperone microsome-associated 60 kDa protein precursor (Microsomal stress 70 protein ATPase core) [Danio rerio] 5.0E-92
blast XP_688977 PREDICTED: similar to Stress 70 protein chaperone microsome-associated 60 kDa protein precursor (Microsomal stress 70 protein ATPase core) [Danio rerio] 9.0E-88
Protein Domains
Database ID Description E-Value
Pfam IPR001023 EMBL-EBI Heat shock protein Hsp70 1.9E-108
Pfam IPR001023 EMBL-EBI Heat shock protein Hsp70 1.9E-108
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495808.2 1 7-24
F54C9.2 1 7-24
SNAP00000013419 1 12-29
GENEFINDER00000013449 1 7-24
Sequence
Target Sequence
>C. ELEGANS:192147_S_AT
tcaggctgacgaatgttccatccacaacaatttcacttaatctcaaaacagtcggaaagt
ggaattatgaattaacacgtgacgaatttgaaactttgaatggcgatttactcaaggcaa
ttgaacttccaattacggcagctttagctgatgcaaatttggatactgctgatgtggacg
aaattgtactcgttggcggttcaacacaagttcctgccgtcagaaaaattgtgggcagat
ttttcaaaaagtctgccaattacggtgtcgacccagaacttgccgtcgtaacaggagctt
ctgttcaagctggtgtcatcggtggtggatggcctctacaagtagccgcgatggagttac
cagcaa


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
TCAGGCTGACGAATGTTCCATCCAC 424 623 983 Antisense
TTCCATCCACAACAATTTCACTTAA 447 697 998 Antisense
GAATGGCGATTTACTCAAGGCAATT 257 333 1080 Antisense
AACTTCCAATTACGGCAGCTTTAGC 11 139 1106 Antisense
GGCGGTTCAACACAAGTTCCTGCCG 221 541 1177 Antisense
AGTCTGCCAATTACGGTGTCGACCC 347 61 1232 Antisense
TCGACCCAGAACTTGCCGTCGTAAC 74 601 1250 Antisense
ACTTGCCGTCGTAACAGGAGCTTCT 245 103 1260 Antisense
GTTCAAGCTGGTGTCATCGGTGGTG 619 443 1285 Antisense
GTGGTGGATGGCCTCTACAAGTAGC 503 487 1304 Antisense
GTAGCCGCGATGGAGTTACCAGCAA 696 457 1324 Antisense
Sequence Source Affymetrix Proprietary Database