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[192135_at] enoyl-CoA hydratase/isomerase

Description B0272.4 /REP_DB=WormBase Gene ID /WP=CE00853 /TR=SW:P41942 /GB=CAA86314.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=enoyl-CoA hydratase-isomerase
Cluster 6grp_highest_10_10 > 75      6grp_high1_10_10 > 66      4grp_highest_8_8 > 22      4grp_high_8_8 > 15      6grp_highest_nonorm > 48      6grp_total > 58      4grp_total > 0      6grp_highest_sub > Cu      6grp_high1_sub > Da      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8944
1 2 3 4 range
A 2515.8
(-3)
P
2711.1
(-2.9)
P
3812.4
(-2.1)
P
11218.5
(3)
P
8703
B 2866.5
(-2.7)
P
2507.9
(-3)
P
4116.4
(-1.8)
P
10456.2
(3)
P
7948
C 2612.4
(-2.7)
P
2274.8
(-3)
P
3730.1
(-1.8)
P
9400.7
(3)
P
7126
avr 2664.9
(-2.9)
2497.9
(-3)
3886.3
(-1.9)
10358.5
(3)
7861

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5583
1 2 3 4 5 6 range
A 5203.1
(0)
P
2445.8
(-3)
P
2596.7
(-2.8)
P
3832.5
(-1.5)
P
2995.3
(-2.4)
P
7889.8
(3)
P
5444
B 3765.9
(-0.5)
P
2324.3
(-3)
P
2306.9
(-3)
P
3512.6
(-0.9)
P
2721.3
(-2.3)
P
5758.8
(3)
P
3452
C 3805
(-1.3)
P
2445.8
(-3)
P
3160
(-2.1)
P
3958.4
(-1.2)
P
2610
(-2.8)
P
7361.3
(3)
P
4916
avr 4258
(-0.6)
2405.3
(-3)
2687.9
(-2.6)
3767.8
(-1.2)
2775.5
(-2.5)
7003.3
(3)
4598
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1640
1 2 3 4 range
A 916
(-1.1)
P
588.5
(-3)
P
996.6
(-0.7)
P
1642.2
(3)
P
1054
B 914.1
(-1.9)
P
619.4
(-3)
P
953.9
(-1.8)
P
2228.2
(3)
P
1609
C 864.8
(-1.6)
P
654
(-3)
P
1300
(1.2)
P
1585.1
(3)
P
931
avr 898.3
(-1.6)
620.6
(-3)
1083.5
(-0.7)
1818.5
(3)
1198

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1904
1 2 3 4 5 6 range
A 959.1
(-1.3)
P
869.8
(-1.7)
P
565.5
(-3)
P
972
(-1.2)
P
903.8
(-1.5)
P
1920.9
(3)
P
1355
B 1310.3
(-0.2)
P
773.7
(-2.2)
P
560.2
(-3)
P
930.4
(-1.6)
P
1105.6
(-0.9)
P
2147.4
(3)
P
1587
C 1410.3
(-0.7)
P
746.3
(-3)
P
804.3
(-2.8)
P
796
(-2.8)
P
844.6
(-2.7)
P
2464.2
(3)
P
1718
avr 1226.6
(-0.7)
796.6
(-2.4)
643.3
(-3)
899.5
(-2)
951.3
(-1.8)
2177.5
(3)
1534
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192135_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16762
Sequence Type Exemplar sequence
Representative Public ID B0272.4 NCBI
Target Description B0272.4 /REP_DB=WormBase Gene ID /WP=CE00853 /TR=SW:P41942 /GB=CAA86314.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=enoyl-CoA hydratase-isomerase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077182(11)
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_077182 NCBI Caenorhabditis elegans B0272.4 (B0272.4) mRNA, complete cds. 11/11 None
SNAP00000029670 ENSEMBL cdna:SNAP chromosome:CEL140:X:9428368:9430160:1 11/11 None
GENEFINDER00000029678 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:9428368:9432554:1 10/11 None
B0272.4 ENSEMBL cdna:known chromosome:CEL140:X:9428368:9430160:1 gene:B0272.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:9428367-9430160(+) 100.0 100.0
Public Domain and Genome References
Gene Title enoyl-CoA hydratase/isomerase
Ensembl B0272.4
Entrez Gene 181892 Entrez gene
SwissProt P41942 EMBL-EBI
WormBase CE00853 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA86312 Hypothetical protein B0272.3 [Caenorhabditis elegans] ref|NP_509584.1| B0272.3 [Caenorhabditis elegans] sp|P41938|HCDH2_CAEEL Probable 3-hydroxyacyl-CoA dehydrogenase B0272.3 1.0E-163
blast CAE69821 Hypothetical protein CBG16139 [Caenorhabditis briggsae] 1.0E-151
blast CAA86314 Hypothetical protein B0272.4 [Caenorhabditis elegans] ref|NP_509583.1| B0272.4 [Caenorhabditis elegans] sp|P41942|YKB4_CAEEL Hypothetical protein B0272.4 in chromosome III 1.0E-131
blast CAA86314 Hypothetical protein B0272.4 [Caenorhabditis elegans] ref|NP_509583.1| B0272.4 [Caenorhabditis elegans] sp|P41942|YKB4_CAEEL Hypothetical protein B0272.4 in chromosome III 1.0E-115
blast CAE69822 Hypothetical protein CBG16141 [Caenorhabditis briggsae] 1.0E-109
blast AAH01983 Peci protein [Mus musculus] 6.0E-43
Protein Domains
Database ID Description E-Value
Pfam IPR001753 EMBL-EBI Enoyl-CoA hydratase/isomerase 6.8E-27
Pfam IPR006108 EMBL-EBI 3-hydroxyacyl-CoA dehydrogenase, C-terminal 1.7E-54
Pfam IPR006176 EMBL-EBI 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain 3.3E-115
Sequence
Target Sequence
>C. ELEGANS:192135_AT
aagcgatttttctccagctgagctttcaacattgacagatatccaagaacatggatacaa
actttttgtggacatcttgattgctttcccaaaaccaataattgctttggtaaacggaca
tgccgttggagtatctgtgactatgctgggtgtgatggatgcggtcattgccattgacac
tgcaacgtttgccactccgtttgcagacattggtgtgtgcccagaagcgtgttccagcta
caccctcccaagaattatgggtcatcaaaaagctgccgcactgatgatgttcagcgaaaa
gtttaccgctcacgaggctcatattgcgggattggttacacaaattttaccagctgcgac
atttgaaaaagatgcaaagaaaatcatcgaccgctactcaaagctttctccaattaccat
gaaagtagcaaaagaactgatgagaaccacccaaatcaaagacgaactgctcactgtcaa
ccgaaaagagcaagtgcatttgaatgggatgttttcccacgaggacactattgctcgtct
cact


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
AAGCGATTTTTCTCCAGCTGAGCTT 649 149 207 Antisense
GTGGACATCTTGATTGCTTTCCCAA 705 489 274 Antisense
TGCCATTGACACTGCAACGTTTGCC 57 587 375 Antisense
TGCCACTCCGTTTGCAGACATTGGT 520 585 396 Antisense
AGCTACACCCTCCCAAGAATTATGG 139 71 442 Antisense
AAAAGCTGCCGCACTGATGATGTTC 32 133 474 Antisense
TTACCGCTCACGAGGCTCATATTGC 419 681 509 Antisense
GAAAATCATCGACCGCTACTCAAAG 541 353 585 Antisense
GCTACTCAAAGCTTTCTCCAATTAC 229 303 599 Antisense
AAAGACGAACTGCTCACTGTCAACC 224 123 664 Antisense
CGAGGACACTATTGCTCGTCTCACT 56 251 726 Antisense
Sequence Source Affymetrix Proprietary Database