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[192132_at] PH (pleckstrin homology) domain

Description F38B7.3 /REP_DB=WormBase Gene ID /WP=CE20758 /TR=Q20151 /GB=CAA98477.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=PH (pleckstrin homology) domain
Cluster 6grp_highest_10_10 > 85      6grp_high1_10_10 > 56      4grp_highest_8_8 > 53      4grp_high_8_8 > 17      6grp_highest_nonorm > 91      6grp_total > 56      4grp_total > 31      6grp_highest_sub > Cu      6grp_high1_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 251
1 2 3 4 range
A 369.2
(3)
P 		158.9
(-2.3)
P 		132.7
(-3)
P 		238.2
(-0.3)
P 		237
B 384
(3)
P 		192
(-2.1)
P 		159.8
(-3)
P 		285.2
(0.4)
P 		224
C 306.7
(3)
P 		139.5
(-3)
P 		143.7
(-2.8)
P 		259.5
(1.3)
P 		167
avr 353.3
(3) 		163.5
(-2.5) 		145.4
(-3) 		261
(0.3) 		208

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 123
1 2 3 4 5 6 range
A 207.9
(-1.4)
P 		210.2
(-1.3)
P 		183.3
(-2.8)
P 		180.4
(-3)
P 		182.1
(-2.9)
P 		282.9
(3)
P 		103
B 193.4
(-1)
P 		234
(1.4)
P 		255
(2.6)
P 		160.2
(-3)
P 		180.9
(-1.8)
P 		261.9
(3)
P 		102
C 250.8
(2.4)
P 		222.2
(0.5)
P 		212.7
(-0.1)
P 		166.9
(-3)
P 		187.8
(-1.7)
P 		260.7
(3)
P 		94
avr 217.4
(-0.1) 		222.1
(0.2) 		217
(-0.1) 		169.2
(-3) 		183.6
(-2.1) 		268.5
(3) 		99
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 100
1 2 3 4 range
A 134.4
(3)
P 		34.5
(-3)
P 		34.7
(-3)
P 		34.9
(-3)
P 		100
B 122.5
(3)
P 		47.4
(-2.3)
P 		37
(-3)
P 		60.8
(-1.3)
P 		86
C 101.5
(3)
P 		40.1
(-3)
P 		50.1
(-2)
P 		43.8
(-2.6)
P 		61
avr 119.5
(3) 		40.7
(-3) 		40.6
(-3) 		46.5
(-2.6) 		79

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 64
1 2 3 4 5 6 range
A 38.3
(-3)
P 		74.7
(3)
P 		39.9
(-2.7)
P 		45.8
(-1.8)
P 		54.9
(-0.3)
P 		68.9
(2)
P 		36
B 67.3
(-0.3)
P 		77.9
(0.9)
P 		61.9
(-0.9)
P 		42.4
(-3)
P 		73.5
(0.4)
P 		97.7
(3)
P 		55
C 93
(3)
P 		67.8
(0.5)
P 		54.1
(-0.9)
P 		33.6
(-3)
P 		60.8
(-0.3)
P 		87.3
(2.4)
P 		59
avr 66.2
(0.5) 		73.5
(1.5) 		52
(-1.4) 		40.6
(-3) 		63.1
(0.1) 		84.6
(3) 		44
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192132_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14663
Sequence Type Exemplar sequence
Representative Public ID F38B7.3 NCBI
Target Description F38B7.3 /REP_DB=WormBase Gene ID /WP=CE20758 /TR=Q20151 /GB=CAA98477.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=PH (pleckstrin homology) domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073528(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073528 NCBI Caenorhabditis elegans F38B7.3 (F38B7.3) mRNA, complete cds. 11/11 None
SNAP00000015622 ENSEMBL cdna:SNAP chromosome:CEL140:V:11551910:11553991:1 11/11 None
GENEFINDER00000015629 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:11549759:11553991:1 11/11 None
F38B7.3 ENSEMBL cdna:known chromosome:CEL140:V:11551890:11554133:1 gene:F38B7.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:11551909-11553991(+) 100.0 100.0
Public Domain and Genome References
Gene Title PH (pleckstrin homology) domain
Ensembl F38B7.3
Entrez Gene 179585 Entrez gene
SwissProt Q20151 EMBL-EBI
WormBase CE20758 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA98477 Hypothetical protein F38B7.3 [Caenorhabditis elegans] ref|NP_505929.1| F38B7.3 [Caenorhabditis elegans] 3.0E-80
blast CAA98477 Hypothetical protein F38B7.3 [Caenorhabditis elegans] ref|NP_505929.1| F38B7.3 [Caenorhabditis elegans] 4.0E-80
blast CAE75344 Hypothetical protein CBG23324 [Caenorhabditis briggsae] 2.0E-69
blast CAE75344 Hypothetical protein CBG23324 [Caenorhabditis briggsae] 3.0E-69
blast EAA05358 ENSANGP00000011161 [Anopheles gambiae str. PEST] ref|XP_309622.2| ENSANGP00000011161 [Anopheles gambiae str. PEST] 4.0E-13
Protein Domains
Database ID Description E-Value
Pfam IPR001849 EMBL-EBI Pleckstrin-like 3.7E-15
Pfam IPR001849 EMBL-EBI Pleckstrin-like 3.7E-15
Sequence
Target Sequence 
>C. ELEGANS:192132_AT
tatggacaataccctcaacctggttatccacaaccggctggagttccaacttcttctcac
acaactgtaatcgtcagagatggaggttacggtggtggcggatacggtggatatggagga
ggtggtggaggattcggaagcactgggcttggattcggctctggcatcttggctggaagt
ctccttggctacggattgggatccatgtggggaggacatcacagctacggtggatggggt
ggcggttacggtggaggtggttatggaatggctggtggatattactccgataatgataca
actgttaccaacaactattacaattatggagataatgacaacaatggaggaaacaatcat
agtagtagctcgaatgctattacagatgctcctggaaatgccggtgatcaatctcaacag
cagtcggataataattacggaaatgctaaccaagactcatatgatcaaggaaatgattat
gggaatgactatggaaatgctggttatgactctggagactttggtggcggagactacgga
ggtggtggatatgattcgggagacttcggaggtggtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATGGACAATACCCTCAACCTGGTT 275 653 607 Antisense
CAACCTGGTTATCCACAACCGGCTG 667 183 622 Antisense
CAACCGGCTGGAGTTCCAACTTCTT 44 187 637 Antisense
CAACTTCTTCTCACACAACTGTAAT 151 189 653 Antisense
GAAGCACTGGGCTTGGATTCGGCTC 238 341 743 Antisense
TCTGGCATCTTGGCTGGAAGTCTCC 347 607 766 Antisense
AAGTCTCCTTGGCTACGGATTGGGA 95 155 783 Antisense
GTGGGGAGGACATCACAGCTACGGT 417 487 813 Antisense
ATGCTCCTGGAAATGCCGGTGATCA 443 41 992 Antisense
GCCGGTGATCAATCTCAACAGCAGT 14 285 1006 Antisense
GATTCGGGAGACTTCGGAGGTGGTG 188 431 1159 Antisense
Sequence Source Affymetrix Proprietary Database