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[192127_s_at] neurofilament triplet M protein

Description T04A8.13 /REP_DB=WormBase Gene ID /WP=CE23943 /TR=Q22142 /GB=CAA84732.2 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=neurofilament triplet M protein
Cluster 6grp_highest_10_10 > 04      6grp_high1_10_10 > 21      4grp_highest_8_8 > 55      4grp_high_8_8 > 19      6grp_highest_nonorm > 71      6grp_total > 32      4grp_total > 21      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 176
1 2 3 4 range
A 242.2
(-0)
P 		317
(3)
P 		167.9
(-3)
P 		307.3
(2.6)
P 		149
B 273.6
(3)
P 		146.3
(-2.7)
P 		140.7
(-3)
P 		244.1
(1.7)
P 		133
C 282.8
(1.8)
P 		195.6
(-1.3)
P 		147.4
(-3)
P 		315.1
(3)
P 		168
avr 266.2
(2) 		219.6
(-0) 		152
(-3) 		288.8
(3) 		137

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 248
1 2 3 4 5 6 range
A 374.1
(1.5)
P 		297.2
(-0.4)
P 		437
(3)
P 		317.1
(0.1)
P 		285.2
(-0.7)
P 		188.6
(-3)
P 		248
B 329.2
(-0.5)
P 		374.1
(3)
P 		332
(-0.3)
P 		322.1
(-1)
P 		347.6
(1)
P 		296.5
(-3)
P 		78
C 330.9
(1)
P 		385.2
(3)
P 		350.9
(1.7)
P 		335.9
(1.1)
P 		233.2
(-2.7)
P 		226
(-3)
P 		159
avr 344.7
(1.7) 		352.2
(2.1) 		373.3
(3) 		325
(0.9) 		288.7
(-0.7) 		237
(-3) 		136
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 61
1 2 3 4 range
A 88.2
(3)
P 		68.8
(0.4)
P 		43.9
(-3)
P 		45
(-2.9)
P 		44
B 87.2
(3)
P 		36.1
(-2.6)
P 		32.6
(-3)
P 		52
(-0.9)
P 		55
C 93.6
(3)
P 		56.2
(-2.3)
P 		51.4
(-3)
P 		53.1
(-2.8)
P 		42
avr 89.7
(3) 		53.7
(-1.6) 		42.6
(-3) 		50
(-2.1) 		47

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 95
1 2 3 4 5 6 range
A 69
(-0.7)
P 		105.7
(3)
P 		95.2
(1.9)
P 		80.4
(0.5)
P 		86.1
(1)
P 		45.9
(-3)
P 		60
B 114.5
(0.4)
P 		124.5
(1.3)
P 		80.6
(-3)
P 		85.3
(-2.5)
P 		141.2
(3)
P 		110.5
(-0)
P 		61
C 122.7
(3)
P 		117.5
(2.4)
P 		89.3
(-0.6)
P 		67.6
(-3)
P 		75.5
(-2.1)
P 		75.7
(-2.1)
P 		55
avr 102.1
(0.8) 		115.9
(3) 		88.4
(-1.3) 		77.8
(-2.9) 		100.9
(0.7) 		77.4
(-3) 		39
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192127_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7414
Sequence Type Exemplar sequence
Representative Public ID T04A8.13 NCBI
Target Description T04A8.13 /REP_DB=WormBase Gene ID /WP=CE23943 /TR=Q22142 /GB=CAA84732.2 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=neurofilament triplet M protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065566(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065566 NCBI Caenorhabditis elegans T04A8.13 (T04A8.13) mRNA, complete cds. 11/11 None
SNAP00000041103 ENSEMBL cdna:SNAP chromosome:CEL140:III:4709210:4712489:1 11/11 None
GENEFINDER00000041117 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4709210:4712489:1 11/11 None
T04A8.13 ENSEMBL cdna:known chromosome:CEL140:III:4707116:4712489:1 gene:T04A8.13 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4707115-4712489(+) 87.33 100.0
Public Domain and Genome References
Gene Title neurofilament triplet M protein
Ensembl T04A8.13
Entrez Gene 175619 Entrez gene
SwissProt Q22142 EMBL-EBI
WormBase CE23943 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA84732 Hypothetical protein T04A8.13 [Caenorhabditis elegans] ref|NP_497967.1| T04A8.13 [Caenorhabditis elegans] 0.0
blast CAE71148 Hypothetical protein CBG18003 [Caenorhabditis briggsae] 1.0E-156
blast CAE71148 Hypothetical protein CBG18003 [Caenorhabditis briggsae] 1.0E-133
Protein Domains
Database ID Description E-Value
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 2.0E-7
Sequence
Target Sequence 
>C. ELEGANS:192127_S_AT
gatttcttccaaccatattcatttgatgtctatatgcataaaaccgctataaagtatctt
tggccgttctcattgcttactgtagctcgtcacggactgattgattggttcaagatgcgt
gcaaacgatcgttctctttgtgatcaaatgcttgataaggatcttcgaattctcattctt
catgactcttttgttagccaagaaagaatgcgtgactatattgacaaatgggcacacggt
ggtgtttccgataagttctgttggtgggctataagtactcatctcgaattggaagtagaa
gaagtgattgatcgactaattgtggagtttgttgaagctaaatatggacctaaattgtta
gaatttagacattctaaaggttttatatttcttattttcagctgtattgctgaagagaac
ccgaaaaagccgcaaccagtgcaaactgtaaaaatggtaagatatgtagtgtacagcgcc
ttggatccaacaaagattgcacatttgtacattcgggataaaatggtactcttcgcgaat
actggatgtgctcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTTCTTCCAACCATATTCATTTG 55 429 1768 Antisense
TAAAGTATCTTTGGCCGTTCTCATT 498 641 1817 Antisense
GCCGTTCTCATTGCTTACTGTAGCT 263 283 1830 Antisense
TACTGTAGCTCGTCACGGACTGATT 92 647 1845 Antisense
GCGTGCAAACGATCGTTCTCTTTGT 239 291 1884 Antisense
GGTGGTGTTTCCGATAAGTTCTGTT 75 497 2005 Antisense
GGTGGGCTATAAGTACTCATCTCGA 48 501 2030 Antisense
ACCCGAAAAAGCCGCAACCAGTGCA 483 87 2186 Antisense
TGTACAGCGCCTTGGATCCAACAAA 87 559 2237 Antisense
GGGATAAAATGGTACTCTTCGCGAA 148 497 2282 Antisense
TCTTCGCGAATACTGGATGTGCTCC 305 617 2297 Antisense
Sequence Source Affymetrix Proprietary Database