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[192109_s_at]

Description B0334.3B /REP_DB=WormBase Gene ID /WP=CE02935 /TR=Q17475 /GB=CAA91372.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=Thiamine pyrophosphate enzymes
Cluster 6grp_highest_10_10 > 60      6grp_high1_10_10 > 17      4grp_highest_8_8 > 36      4grp_high_8_8 > 0      6grp_highest_nonorm > 05      6grp_total > 07      4grp_total > 07      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1786
1 2 3 4 range
A 6584.5
(3)
P 		6208.8
(-3)
P 		6397.2
(0)
P 		6564.2
(2.7)
P 		376
B 6895.6
(3)
P 		5110
(-3)
P 		6749.3
(2.5)
P 		6303.4
(1)
P 		1786
C 6516.3
(3)
P 		5331.3
(-3)
P 		6172.5
(1.3)
P 		6319.1
(2)
P 		1185
avr 6665.5
(3) 		5550
(-3) 		6439.7
(1.8) 		6395.6
(1.5) 		1116

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6340
1 2 3 4 5 6 range
A 9470.6
(3)
P 		6613.6
(0)
P 		5131.7
(-1.5)
P 		3945.8
(-2.8)
P 		3711.4
(-3)
P 		4644.8
(-2)
P 		5759
B 9708.4
(3)
P 		6056.3
(-0.6)
P 		4563.4
(-2.1)
P 		3983.5
(-2.6)
P 		4018.7
(-2.6)
P 		3609.1
(-3)
P 		6099
C 9653
(3)
P 		5313.5
(-1.1)
P 		5733.4
(-0.7)
P 		3420.4
(-3)
P 		4527
(-1.9)
P 		3368.8
(-3)
P 		6284
avr 9610.7
(3) 		5994.5
(-0.7) 		5142.8
(-1.6) 		3783.2
(-3) 		4085.7
(-2.7) 		3874.2
(-2.9) 		5828
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1437
1 2 3 4 range
A 2397.4
(3)
P 		1347.8
(-1.4)
P 		1672.3
(-0)
P 		960.9
(-3)
P 		1437
B 2198.9
(3)
P 		1262.1
(-3)
P 		1564
(-1.1)
P 		1343.3
(-2.5)
P 		937
C 2157.1
(3)
P 		1532.8
(-0.4)
P 		2151.3
(3)
P 		1065.5
(-3)
P 		1092
avr 2251.1
(3) 		1380.9
(-1.6) 		1795.9
(0.6) 		1123.2
(-3) 		1128

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2890
1 2 3 4 5 6 range
A 1745.8
(0.3)
P 		2352.1
(3)
P 		1117.6
(-2.5)
P 		1000.7
(-3)
P 		1119.9
(-2.5)
P 		1130.9
(-2.4)
P 		1351
B 3378.1
(3)
P 		2015.9
(-0.5)
P 		1108.1
(-2.9)
P 		1055.1
(-3)
P 		1632.8
(-1.5)
P 		1345.8
(-2.2)
P 		2323
C 3578
(3)
P 		1621.3
(-1.1)
P 		1459.3
(-1.4)
P 		687.8
(-3)
P 		1464.9
(-1.4)
P 		1127.7
(-2.1)
P 		2890
avr 2900.6
(3) 		1996.4
(0.3) 		1228.3
(-2.1) 		914.5
(-3) 		1405.9
(-1.5) 		1201.5
(-2.1) 		1986
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192109_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2838
Sequence Type Exemplar sequence
Representative Public ID B0334.3B NCBI
Target Description B0334.3B /REP_DB=WormBase Gene ID /WP=CE02935 /TR=Q17475 /GB=CAA91372.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=Thiamine pyrophosphate enzymes
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064054(11), NM_064053(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064054 NCBI Caenorhabditis elegans B0334.3b (B0334.3) mRNA, complete cds. 11/11 A 
NM_064053 NCBI Caenorhabditis elegans B0334.3a (B0334.3) mRNA, complete cds. 11/11 A 
SNAP00000019013 ENSEMBL cdna:SNAP chromosome:CEL140:II:11489613:11492868:-1 11/11 None
GENEFINDER00000019033 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:11489613:11492868:-1 11/11 None
B0334.3b ENSEMBL cdna:known chromosome:CEL140:II:11489520:11492539:-1 gene:B0334.3 11/11 A 
B0334.3a.1 ENSEMBL cdna:known chromosome:CEL140:II:11489613:11492926:-1 gene:B0334.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:11489609-11492207(-) 100.0 100.0
Public Domain and Genome References
Ensembl B0334.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA91372 Hypothetical protein B0334.3b [Caenorhabditis elegans] ref|NP_496455.1| B0334.3b [Caenorhabditis elegans] 0.0
blast CAA91381 Hypothetical protein B0334.3a [Caenorhabditis elegans] ref|NP_496454.1| B0334.3a [Caenorhabditis elegans] 0.0
blast CAE59489 Hypothetical protein CBG02874 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000399 EMBL-EBI Pyruvate decarboxylase 2.8E-28
Pfam IPR000399 EMBL-EBI Pyruvate decarboxylase 4.5E-46
Sequence
Target Sequence 
>C. ELEGANS:192109_S_AT
gatcactcaactccactgaattactacgcagcatatcaaccaattcgtgaattcctcgcc
aacaatgacgtcattgtgatcaacgaaggagccaacacaatggatatcggccgaaccatg
atgccttcccgtctcccgaagcgtcgcctcgacgccggaacctttggaacaatgggagtc
ggtcatggattctccctcgccgccgcactctgggcaagagatcacagtccgaagactaag
gttctcgtggttcaaggagacagtgctttcggattctctgccatggaactcgagacaatt
gctcgatacaatcttccagtcgtcactgttatcattaacaactctggaatctatcgtgga
ctcctgccagaagatgataaggcaatcgaaggagaccgtactcttgctcttccagttctc
tcgctaactgctgaatgtcgttatgaagaaatgtgcaaagctttcggaggagctggaact
gttgttagaactgttccagaaatcaaggcagctctcgagaaggcattccaaaaaaccgac
ggaccaactgttatcaatgctctcat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCACTCAACTCCACTGAATTACT 175 419 1291 Antisense
GATATCGGCCGAACCATGATGCCTT 315 425 1393 Antisense
TCGACGCCGGAACCTTTGGAACAAT 644 595 1439 Antisense
GGGAGTCGGTCATGGATTCTCCCTC 188 495 1464 Antisense
GACTAAGGTTCTCGTGGTTCAAGGA 223 363 1524 Antisense
TCGGATTCTCTGCCATGGAACTCGA 39 605 1559 Antisense
CAATCTTCCAGTCGTCACTGTTATC 432 181 1599 Antisense
TCTATCGTGGACTCCTGCCAGAAGA 267 615 1640 Antisense
TCGAAGGAGACCGTACTCTTGCTCT 665 595 1676 Antisense
GTTCTCTCGCTAACTGCTGAATGTC 519 443 1705 Antisense
GACCAACTGTTATCAATGCTCTCAT 87 385 1832 Antisense
Sequence Source Affymetrix Proprietary Database