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[192105_at] Archael histone like

Description F53A2.5 /REP_DB=WormBase Gene ID /WP=CE16097 /GEN=dro-1 /TR=O45550 /GB=CAB04453.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Archael histone like
Cluster 6grp_total > 42      4grp_total > 20      6grp_cgc > 81      6grp_cgc_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 56
1 2 3 4 range
A 32.2
(-3)
A 		61
(3)
A 		46.8
(0)
A 		46.1
(-0.1)
A 		29
B 42.4
(3)
A 		22.4
(-3)
A 		29.8
(-0.8)
A 		38.6
(1.9)
A 		20
C 17.3
(0.6)
A 		7.2
(-2.5)
A 		5.5
(-3)
A 		25.2
(3)
A 		20
avr 30.6
(-0.9) 		30.2
(-1.2) 		27.4
(-3) 		36.6
(3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 46
1 2 3 4 5 6 range
A 9.5
(-2.7)
A 		28.7
(0.2)
A 		38.4
(1.7)
A 		46.9
(3)
A 		7.5
(-3)
A 		13.4
(-2.1)
A 		39
B 24.7
(2.1)
A 		28.2
(3)
A 		18.8
(0.6)
A 		10.4
(-1.5)
A 		8.4
(-2)
A 		4.3
(-3)
A 		24
C 41.9
(2.5)
A 		2.7
(-2.8)
A 		45.8
(3)
A 		7.5
(-2.2)
A 		1.3
(-3)
A 		11.1
(-1.7)
A 		45
avr 25.4
(1.1) 		19.9
(-0) 		34.3
(3) 		21.6
(0.3) 		5.7
(-3) 		9.6
(-2.2) 		29
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 11.7
(1.6)
A 		13.2
(3)
A 		12.2
(2.1)
A 		6.7
(-3)
A 		7
B 13.5
(3)
A 		5.5
(-3)
A 		6.9
(-2)
A 		8.2
(-1)
A 		8
C 5.7
(3)
A 		2.1
(-2.7)
A 		1.9
(-3)
A 		4.2
(0.6)
A 		4
avr 10.3
(3) 		6.9
(-2.2) 		7
(-2.1) 		6.4
(-3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 1.8
(-3)
A 		10.2
(2)
A 		8.4
(0.9)
A 		11.9
(3)
A 		2.3
(-2.7)
A 		3.3
(-2.1)
A 		10
B 8.6
(2.4)
A 		9.4
(3)
A 		4.6
(-0.7)
A 		2.8
(-2.1)
A 		3.4
(-1.6)
A 		1.6
(-3)
A 		8
C 15.5
(3)
A 		0.8
(-2.8)
A 		11.7
(1.5)
A 		1.5
(-2.6)
A 		0.4
(-3)
A 		3.7
(-1.7)
A 		15
avr 8.6
(3) 		6.8
(1.4) 		8.2
(2.6) 		5.4
(0.1) 		2
(-3) 		2.9
(-2.2) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192105_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7202
Sequence Type Exemplar sequence
Representative Public ID F53A2.5 NCBI
Target Description F53A2.5 /REP_DB=WormBase Gene ID /WP=CE16097 /GEN=dro-1 /TR=O45550 /GB=CAB04453.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Archael histone like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067349(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067349 NCBI Caenorhabditis elegans DR1 (One) transcription factor family member (dro-1) (dro-1) mRNA, complete cds. 11/11 None
SNAP00000020109 ENSEMBL cdna:SNAP chromosome:CEL140:III:13342239:13342836:1 10/11 None
GENEFINDER00000020116 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:13342239:13342836:1 10/11 None
F53A2.5 ENSEMBL cdna:known chromosome:CEL140:III:13342200:13342836:1 gene:F53A2.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:13342195-13342832(+) 100.0 100.0
Public Domain and Genome References
Gene Title Archael histone like
Gene Symbol dro-1
Ensembl F53A2.5
Entrez Gene 191629 Entrez gene
SwissProt O45550 EMBL-EBI
WormBase CE16097 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:250543_AT TATA-binding protein-associated phosphoprotein Dr1 protein, putative at
CANINE:1588924_AT similar to down-regulator of transcription 1 cfa
CANINE:1587870_AT similar to down-regulator of transcription 1 cfa
CANINE_2:CFA.3926.1.S1_AT similar to down-regulator of transcription 1 cfa
CANINE_2:CFA.6499.1.A1_AT similar to down-regulator of transcription 1 cfa
CANINE_2:CFAAFFX.30839.1.S1_S_AT similar to down-regulator of transcription 1 cfa
DROSOPHILA_2:1632384_AT dm
DROSGENOME1:152652_AT dm
CHICKEN:GGAAFFX.11384.1.S1_S_AT down-regulator of transcription 1, TBP-binding (negative cofactor 2) gga
HG-U95AV2:211_AT down-regulator of transcription 1, TBP-binding (negative cofactor 2) hs
HC-G110:211_AT down-regulator of transcription 1, TBP-binding (negative cofactor 2) hs
HG-U133_PLUS_2:209188_X_AT down-regulator of transcription 1, TBP-binding (negative cofactor 2) hs
HG-U133A:209188_X_AT down-regulator of transcription 1, TBP-binding (negative cofactor 2) hs
HG-U133A_2:209188_X_AT down-regulator of transcription 1, TBP-binding (negative cofactor 2) hs
HG-U133_PLUS_2:207654_X_AT down-regulator of transcription 1, TBP-binding (negative cofactor 2) hs
HG-U133A:207654_X_AT down-regulator of transcription 1, TBP-binding (negative cofactor 2) hs
HG-U133A_2:207654_X_AT down-regulator of transcription 1, TBP-binding (negative cofactor 2) hs
HG-U133_PLUS_2:216652_S_AT down-regulator of transcription 1, TBP-binding (negative cofactor 2) hs
HG-U133A_2:216652_S_AT down-regulator of transcription 1, TBP-binding (negative cofactor 2) hs
HG-U133A:216652_S_AT down-regulator of transcription 1, TBP-binding (negative cofactor 2) hs
HU35KSUBC:RC_AA132007_F_AT down-regulator of transcription 1, TBP-binding (negative cofactor 2) hs
HU35KSUBC:RC_AA132007_I_AT down-regulator of transcription 1, TBP-binding (negative cofactor 2) hs
HU35KSUBD:RC_AA490227_AT down-regulator of transcription 1, TBP-binding (negative cofactor 2) hs
HUGENEFL:M97388_AT down-regulator of transcription 1, TBP-binding (negative cofactor 2) hs
U133_X3P:G4503380_3P_S_AT down-regulator of transcription 1, TBP-binding (negative cofactor 2) hs
U133_X3P:HS.16697.1.S2_3P_AT down-regulator of transcription 1, TBP-binding (negative cofactor 2) hs
HG-U133A_2:209187_AT down-regulator of transcription 1, TBP-binding (negative cofactor 2) hs
HG-FOCUS:209187_AT down-regulator of transcription 1, TBP-binding (negative cofactor 2) hs
HG-U133_PLUS_2:209187_AT down-regulator of transcription 1, TBP-binding (negative cofactor 2) hs
HG-U133A:209187_AT down-regulator of transcription 1, TBP-binding (negative cofactor 2) hs
HG-U95AV2:32621_AT down-regulator of transcription 1, TBP-binding (negative cofactor 2) hs
HG-U95B:52774_AT down-regulator of transcription 1, TBP-binding (negative cofactor 2) hs
HG-U95B:58126_R_AT down-regulator of transcription 1, TBP-binding (negative cofactor 2) hs
HG-U133_PLUS_2:216653_AT Down-regulator of transcription 1, TBP-binding (negative cofactor 2) hs
HG-U133A_2:216653_AT Down-regulator of transcription 1, TBP-binding (negative cofactor 2) hs
HG-U133A:216653_AT Down-regulator of transcription 1, TBP-binding (negative cofactor 2) hs
MG-U74BV2:110634_AT down-regulator of transcription 1 mm
MG-U74AV2:160449_AT down-regulator of transcription 1 mm
MG-U74CV2:168968_R_AT down-regulator of transcription 1 mm
MOE430A:1416018_AT down-regulator of transcription 1 mm
MOUSE430A_2:1416018_AT down-regulator of transcription 1 mm
MOUSE430_2:1416018_AT down-regulator of transcription 1 mm
MOE430A:1416019_AT down-regulator of transcription 1 mm
MOUSE430A_2:1416019_AT down-regulator of transcription 1 mm
MOUSE430_2:1416019_AT down-regulator of transcription 1 mm
MU19KSUBC:TC36598_AT Down-regulator of transcription 1, mRNA (cDNA clone MGC:18680 IMAGE:4222118) mm
RG-U34B:RC_AI012637_AT down-regulator of transcription 1 (predicted) rn
RAE230A:1372453_AT down-regulator of transcription 1 (predicted) rn
RAT230_2:1372453_AT down-regulator of transcription 1 (predicted) rn
RAE230B:1384005_AT down-regulator of transcription 1 (predicted) rn
RAT230_2:1384005_AT down-regulator of transcription 1 (predicted) rn
YEAST_2:1770660_AT Beta subunit of the NC2 dimeric histone-fold complex; represses RNA polymerase II transcription through binding to TBP and inhibition of TFIIA and TFIIB; homologous to the Dr1 subunit of the mammalian NC2 (negative cofactor2) Sc
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04453 Hypothetical protein F53A2.5 [Caenorhabditis elegans] ref|NP_499750.1| DR1 (One) transcription factor family member (dro-1) [Caenorhabditis elegans] 2.0E-86
blast CAB04453 Hypothetical protein F53A2.5 [Caenorhabditis elegans] ref|NP_499750.1| DR1 (One) transcription factor family member (dro-1) [Caenorhabditis elegans] 3.0E-78
blast CAE56548 Hypothetical protein CBG24280 [Caenorhabditis briggsae] 8.0E-72
blast CAE56548 Hypothetical protein CBG24280 [Caenorhabditis briggsae] 1.0E-71
blast XP_392721 PREDICTED: similar to ENSANGP00000017122 [Apis mellifera] 5.0E-28
Protein Domains
Database ID Description E-Value
Pfam IPR003958 EMBL-EBI Transcription factor CBF/NF-Y/archaeal histone 1.2E-25
Pfam IPR003958 EMBL-EBI Transcription factor CBF/NF-Y/archaeal histone 1.2E-25
Sequence
Target Sequence 
>C. ELEGANS:192105_AT
gtgcttcaacacaattttcagactcttcacagatatggatgaggacaatgaaatcggact
tccacagaaaggtatcaaccaaattatcaaagaagttgttcctgaaatgcgaatcgccaa
cgagtcgagagatatgatcaatgcttgctgcgttgagttcgtgaagcatattgcgagaga
agcacaacgaattgcgagccaggatcagagaaaaacaatatatcacgaacatgttcagaa
ggcactgcagaatttaggattcacaccagattatctcgaggccgccgacagtgttctcga
tgcctgcaaggttgaagccgagaagaagctgcgaaggaagaactctcgtcttgaaaaatg
tggtattccagaggaaaaattgtacgaaatgcaacaagaattaattcagaaggcacgcca
acaagaaattgagcagcagcttgctcaccaacaagcacaagccgcggcggtagcagccgc
aaacgcacaaatgtttggaggaccatcagagggctctttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGCTTCAACACAATTTTCAGACTC 409 479 18 Antisense
GTTGTTCCTGAAATGCGAATCGCCA 100 439 112 Antisense
GCGAATCGCCAACGAGTCGAGAGAT 102 289 126 Antisense
AATGCTTGCTGCGTTGAGTTCGTGA 31 167 157 Antisense
GAAGCACAACGAATTGCGAGCCAGG 543 341 196 Antisense
TCACACCAGATTATCTCGAGGCCGC 196 629 278 Antisense
TGTTCTCGATGCCTGCAAGGTTGAA 291 561 309 Antisense
GCAGCAGCTTGCTCACCAACAAGCA 443 313 450 Antisense
CAACAAGCACAAGCCGCGGCGGTAG 25 187 466 Antisense
GTAGCAGCCGCAAACGCACAAATGT 378 457 487 Antisense
TGGAGGACCATCAGAGGGCTCTTTG 492 549 513 Antisense
Sequence Source Affymetrix Proprietary Database