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[192081_s_at]

Description K08E3.3B /REP_DB=WormBase Gene ID /WP=CE18867 /TR=Q9XUS7 /GB=CAB04595.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=EF hand, Src homology domain 3
Cluster 6grp_total > 87      4grp_total > 32      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 112
1 2 3 4 range
A 82.2
(-0.6)
A 		54.4
(-3)
A 		125.2
(3)
A 		111.7
(1.9)
A 		71
B 59.2
(-3)
A 		94.4
(-0.9)
A 		161.5
(3)
A 		81.4
(-1.7)
A 		102
C 68.7
(-3)
A 		89.4
(-1.7)
A 		114
(-0.2)
A 		166.8
(3)
A 		98
avr 70
(-3) 		79.4
(-2.1) 		133.6
(3) 		120
(1.7) 		64

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 413
1 2 3 4 5 6 range
A 103.9
(-2.5)
A 		72.3
(-3)
A 		87.6
(-2.8)
A 		172.7
(-1.5)
A 		282.6
(0.1)
A 		485.1
(3)
P 		413
B 84
(-3)
A 		82.7
(-3)
A 		111.7
(-2.4)
A 		120.1
(-2.2)
A 		234.1
(0)
A 		381.5
(3)
P 		299
C 83.5
(-2.9)
A 		77.3
(-3)
A 		103.4
(-2.6)
A 		85.7
(-2.9)
A 		274.4
(0.2)
M 		449.1
(3)
P 		372
avr 90.5
(-2.8) 		77.4
(-3) 		100.9
(-2.6) 		126.2
(-2.2) 		263.7
(0.1) 		438.6
(3) 		361
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 28
1 2 3 4 range
A 29.9
(2.2)
A 		11.8
(-3)
A 		32.7
(3)
A 		16.3
(-1.7)
A 		21
B 18.9
(-2.5)
A 		23.3
(-1.2)
A 		37.4
(3)
A 		17.3
(-3)
A 		20
C 22.7
(-3)
A 		25.7
(-1.9)
A 		39.7
(3)
A 		28.1
(-1.1)
A 		17
avr 23.8
(-1.7) 		20.3
(-3) 		36.6
(3) 		20.6
(-2.9) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 133
1 2 3 4 5 6 range
A 19.1
(-3)
A 		25.7
(-2.6)
A 		19.1
(-3)
A 		43.8
(-1.5)
A 		85.3
(1)
A 		118.1
(3)
P 		99
B 29.2
(-2.9)
A 		27.5
(-3)
A 		27.1
(-3)
A 		31.8
(-2.8)
A 		95.1
(0.5)
A 		142.3
(3)
P 		115
C 31
(-2.4)
A 		23.6
(-2.7)
A 		26.3
(-2.6)
A 		17.2
(-3)
A 		88.8
(0.2)
M 		150.3
(3)
P 		133
avr 26.4
(-2.9) 		25.6
(-2.9) 		24.2
(-3) 		30.9
(-2.6) 		89.7
(0.5) 		136.9
(3) 		113
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192081_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6214
Sequence Type Exemplar sequence
Representative Public ID K08E3.3B NCBI
Target Description K08E3.3B /REP_DB=WormBase Gene ID /WP=CE18867 /TR=Q9XUS7 /GB=CAB04595.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=EF hand, Src homology domain 3
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067438(11), NM_067437(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067438 NCBI Caenorhabditis elegans K08E3.3a (K08E3.3) mRNA, complete cds. 11/11 A 
NM_067437 NCBI Caenorhabditis elegans K08E3.3b (K08E3.3) mRNA, complete cds. 11/11 A 
SNAP00000031962 ENSEMBL cdna:SNAP chromosome:CEL140:III:13752038:13758864:1 11/11 None
GENEFINDER00000031975 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:13754400:13758864:1 10/11 None
K08E3.3a ENSEMBL cdna:known chromosome:CEL140:III:13755458:13758928:1 gene:K08E3.3 11/11 A 
K08E3.3b ENSEMBL cdna:known chromosome:CEL140:III:13755515:13758943:1 gene:K08E3.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:13754395-13758860(+) 95.21 100.0
Public Domain and Genome References
Ensembl K08E3.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04591 Hypothetical protein K08E3.3a [Caenorhabditis elegans] ref|NP_499839.2| K08E3.3a [Caenorhabditis elegans] 0.0
blast CAB04595 Hypothetical protein K08E3.3b [Caenorhabditis elegans] ref|NP_499838.2| K08E3.3b [Caenorhabditis elegans] 0.0
blast CAE71363 Hypothetical protein CBG18267 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001452 EMBL-EBI SH3 9.0E-10
Pfam IPR001060 EMBL-EBI Cdc15/Fes/CIP4 2.0E-25
Pfam IPR001452 EMBL-EBI SH3 5.8E-8
Pfam IPR001452 EMBL-EBI SH3 9.0E-10
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 5.6E-4
Pfam IPR001452 EMBL-EBI SH3 9.0E-10
Pfam IPR001060 EMBL-EBI Cdc15/Fes/CIP4 2.0E-25
Sequence
Target Sequence 
>C. ELEGANS:192081_S_AT
cggaaccacttatatcgtacgctaaaaagatagagaagctcaaaatggatatacataacc
tcaaggagttctacgcgatgcttgaaatgagtgtggaagaaggccaggagcgctcttttg
gcggccgtgatacccctgacacaacgagaagcatgtcaggcagttcgacaaatcaatcat
catccaaaactatcgaagacgtgctgtctggcgaagcgggaaattcttcatcagcagatg
actcgtcgaagaatatcctccgccaactgtttacaactccgaaacggcttatctcctcgc
caaaaacctcgaagtcttcaactccgacgccactacgacgaagagccgaaatctcatcac
caaagattctcagatcctcatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGGAACCACTTATATCGTACGCTAA 597 209 1745 Antisense
ACCTCAAGGAGTTCTACGCGATGCT 37 89 1802 Antisense
AGAAGGCCAGGAGCGCTCTTTTGGC 312 73 1842 Antisense
TGATACCCCTGACACAACGAGAAGC 338 459 1872 Antisense
ACGTGCTGTCTGGCGAAGCGGGAAA 518 93 1943 Antisense
TGACTCGTCGAAGAATATCCTCCGC 614 569 1983 Antisense
CAACTCCGAAACGGCTTATCTCCTC 528 187 2018 Antisense
TTATCTCCTCGCCAAAAACCTCGAA 614 677 2033 Antisense
AACCTCGAAGTCTTCAACTCCGACG 476 145 2049 Antisense
ACGCCACTACGACGAAGAGCCGAAA 695 89 2071 Antisense
CACCAAAGATTCTCAGATCCTCATT 486 201 2102 Antisense
Sequence Source Affymetrix Proprietary Database