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[192070_at] chitin synthase like

Description T25G3.2 /REP_DB=WormBase Gene ID /WP=CE06511 /GEN=chi-1 /TR=Q22791 /GB=CAA96688.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=chitin synthase like
Cluster 6grp_total > 88      4grp_total > 01      6grp_cgc > 59      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 130
1 2 3 4 range
A 2.5
(-3)
A 		16.4
(-2.3)
A 		31.8
(-1.5)
A 		118.1
(3)
P 		116
B 42.9
(3)
A 		10.6
(-3)
A 		11.1
(-2.9)
A 		39.9
(2.4)
A 		32
C 25
(-2.2)
A 		9.6
(-3)
A 		43.3
(-1.4)
A 		132.4
(3)
P 		123
avr 23.5
(-2.2) 		12.2
(-3) 		28.7
(-1.8) 		96.8
(3) 		85

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1281
1 2 3 4 5 6 range
A 3.6
(-3)
A 		15
(-2.9)
A 		56
(-2.7)
P 		133.3
(-2.3)
P 		627
(0.3)
P 		1149
(3)
P 		1145
B 6.2
(-3)
A 		48.1
(-2.8)
A 		32.3
(-2.9)
P 		130.6
(-2.3)
P 		513.6
(-0.3)
P 		1121
(3)
P 		1115
C 3.2
(-3)
A 		47.3
(-2.8)
A 		55.3
(-2.8)
P 		142.1
(-2.3)
P 		557.3
(-0.4)
P 		1284.4
(3)
P 		1281
avr 4.3
(-3) 		36.8
(-2.8) 		47.9
(-2.8) 		135.3
(-2.3) 		566
(-0.1) 		1184.8
(3) 		1181
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 0.9
(-3)
A 		3.6
(-2)
A 		8.3
(-0.3)
A 		17.3
(3)
P 		16
B 13.7
(3)
A 		2.6
(-3)
A 		2.6
(-3)
A 		8.5
(0.2)
A 		11
C 8.3
(-1.3)
A 		2.8
(-3)
A 		15.1
(0.8)
A 		22.3
(3)
P 		20
avr 7.6
(-0.9) 		3
(-3) 		8.7
(-0.4) 		16
(3) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 429
1 2 3 4 5 6 range
A 0.7
(-3)
A 		5.3
(-2.9)
A 		12.2
(-2.8)
P 		33.8
(-2.3)
P 		189.2
(1.1)
P 		279.8
(3)
P 		279
B 2.2
(-3)
A 		16
(-2.8)
A 		7.9
(-2.9)
P 		34.6
(-2.5)
P 		208.7
(-0)
P 		418
(3)
P 		416
C 1.2
(-3)
A 		14.4
(-2.8)
A 		14.1
(-2.8)
P 		28.6
(-2.6)
P 		180.3
(-0.5)
P 		429.9
(3)
P 		429
avr 1.4
(-3) 		11.9
(-2.8) 		11.4
(-2.8) 		32.3
(-2.5) 		192.7
(0.1) 		375.9
(3) 		375
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192070_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1776
Sequence Type Exemplar sequence
Representative Public ID T25G3.2 NCBI
Target Description T25G3.2 /REP_DB=WormBase Gene ID /WP=CE06511 /GEN=chi-1 /TR=Q22791 /GB=CAA96688.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=chitin synthase like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059712(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059712 NCBI Caenorhabditis elegans CHitin Synthase family member (chs-1) (chs-1) mRNA, complete cds. 11/11 None
SNAP00000010743 ENSEMBL cdna:SNAP chromosome:CEL140:I:7562790:7567583:1 11/11 None
GENEFINDER00000010747 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:7563128:7567583:1 11/11 None
T25G3.2.2 ENSEMBL cdna:known chromosome:CEL140:I:7562852:7568043:1 gene:T25G3.2 11/11 None
T25G3.2.1 ENSEMBL cdna:known chromosome:CEL140:I:7562852:7567844:1 gene:T25G3.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:7562782-7567514(+) 100.0 100.0
Public Domain and Genome References
Gene Title chitin synthase like
Gene Symbol chs-1
Ensembl T25G3.2
Entrez Gene 172508 Entrez gene
SwissProt Q22791 EMBL-EBI
WormBase CE06511 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_492113 CHitin Synthase family member (chs-1) [Caenorhabditis elegans] 0.0
blast CAE66574 Hypothetical protein CBG11891 [Caenorhabditis briggsae] 0.0
blast AAG39382 chitin synthase [Dirofilaria immitis] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004835 EMBL-EBI Fungal chitin synthase 1.8E-7
Pfam IPR004835 EMBL-EBI Fungal chitin synthase 3.6E-6
Pfam IPR004835 EMBL-EBI Fungal chitin synthase 1.8E-7
Pfam IPR004835 EMBL-EBI Fungal chitin synthase 3.6E-6
Pfam IPR004835 EMBL-EBI Fungal chitin synthase 1.8E-7
Pfam IPR004835 EMBL-EBI Fungal chitin synthase 3.6E-6
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_492113.1 14 95-117,252-274,278-300,326-348,375-397,404-421,455-477,895-917,927-949,956-978,982-1004,1011-1033,1208-1227,1268-1290
T25G3.2.2 14 95-117,252-274,278-300,326-348,375-397,404-421,455-477,895-917,927-949,956-978,982-1004,1011-1033,1208-1227,1268-1290
T25G3.2.1 14 95-117,252-274,278-300,326-348,375-397,404-421,455-477,895-917,927-949,956-978,982-1004,1011-1033,1208-1227,1268-1290
SNAP00000010743 14 87-109,244-266,270-292,318-340,367-389,396-413,447-469,887-909,919-941,948-970,974-996,1003-1025,1200-1219,1260-1282
GENEFINDER00000010747 14 37-59,194-216,220-242,268-290,317-339,346-363,397-419,837-859,869-891,898-920,924-946,953-975,1150-1169,1210-1232
Sequence
Target Sequence 
>C. ELEGANS:192070_AT
gaaaccgatcaagacgacgccagctgaaatgaaagccgtcgccgaaggattggcttctct
acgaaatcagattgctttcactattcttctcgttaattctcttcttgctcttgccatctt
tttgattcagaaacacaaaaatgtgctcagcatcaagttctcgccaatcaaaaacttccg
atggacgaaaatgaatgagatgactggacaatacgaggaaaccgatgaaccattaaaaat
agatccacttggaatgggaattgttgttttccttctaattattctttttgttcaaactct
cggaatgcttctccatcgacttaacactatgataggagctttccaagaagtgaaaaattt
gtacgaatacggagtttcaccagtgataaatacaaagaatgatgatgagaggattatgaa
taatgctcgtctaatgattaattctctcggtgtcagtactggacacgctgccgacggcta
tacaaggcatcgcggagaggaatccgataccggcaatgttctctacaaacttcaaaaggc
tcgtctcgcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAACCGATCAAGACGACGCCAGCT 87 359 3567 Antisense
GTCGCCGAAGGATTGGCTTCTCTAC 594 473 3604 Antisense
CAGATTGCTTTCACTATTCTTCTCG 120 207 3634 Antisense
ACTATTCTTCTCGTTAATTCTCTTC 175 103 3646 Antisense
AGCATCAAGTTCTCGCCAATCAAAA 336 77 3715 Antisense
TGTTGTTTTCCTTCTAATTATTCTT 306 563 3828 Antisense
TCAAACTCTCGGAATGCTTCTCCAT 606 629 3858 Antisense
TAATTCTCTCGGTGTCAGTACTGGA 223 635 4005 Antisense
GCCGACGGCTATACAAGGCATCGCG 528 283 4036 Antisense
TCCGATACCGGCAATGTTCTCTACA 348 595 4069 Antisense
AAACTTCAAAAGGCTCGTCTCGCAA 308 127 4093 Antisense
Sequence Source Affymetrix Proprietary Database