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[192066_at]

Description Y58G8A.3 /REP_DB=WormBase Gene ID /WP=CE24480 /TR=Q9N4R5 /GB=AAF60815.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=seven trans-membrane receptor
Cluster 6grp_highest_10_10 > 40      6grp_high1_10_10 > 15      4grp_highest_8_8 > 54      4grp_high_8_8 > 09      6grp_highest_nonorm > 60      6grp_total > 05      4grp_total > 22      6grp_highest_sub > De      6grp_high1_sub > De      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 261
1 2 3 4 range
A 492.7
(3)
P 		235
(-3)
P 		248.1
(-2.7)
P 		444.8
(1.9)
P 		258
B 391.5
(2.4)
P 		311.7
(-0.3)
P 		231.4
(-3)
P 		408.9
(3)
P 		178
C 454.7
(3)
P 		350.8
(-1.5)
P 		316.7
(-3)
P 		396
(0.4)
P 		138
avr 446.3
(3) 		299.2
(-1.9) 		265.4
(-3) 		416.6
(2) 		181

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 397
1 2 3 4 5 6 range
A 523.4
(3)
P 		476.3
(2.1)
P 		244.8
(-2.5)
P 		293.6
(-1.5)
P 		247.3
(-2.4)
P 		217
(-3)
P 		306
B 515.2
(3)
P 		416.7
(1.1)
P 		255
(-2.1)
P 		279.8
(-1.6)
P 		278
(-1.6)
P 		206.9
(-3)
P 		308
C 577.4
(3)
P 		434.5
(0.8)
P 		336.9
(-0.6)
P 		319.2
(-0.9)
P 		282
(-1.5)
P 		180.1
(-3)
P 		397
avr 538.7
(3) 		442.5
(1.3) 		278.9
(-1.6) 		297.5
(-1.3) 		269.1
(-1.8) 		201.3
(-3) 		337
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 128
1 2 3 4 range
A 179.4
(3)
P 		51
(-3)
P 		64.9
(-2.4)
P 		65.1
(-2.3)
P 		128
B 124.8
(3)
P 		77
(-1)
P 		53.6
(-3)
P 		87.1
(-0.2)
P 		71
C 150.5
(3)
P 		100.8
(-0.6)
P 		110.4
(0.1)
P 		66.8
(-3)
P 		84
avr 151.6
(3) 		76.3
(-2.7) 		76.3
(-2.7) 		73
(-3) 		79

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 161
1 2 3 4 5 6 range
A 96.5
(-0.8)
P 		169.4
(3)
P 		53.3
(-3)
P 		74.5
(-1.9)
P 		74.6
(-1.9)
P 		52.8
(-3)
P 		117
B 179.3
(3)
P 		138.7
(0.9)
P 		61.9
(-3)
P 		74.1
(-2.4)
P 		112.9
(-0.4)
P 		77.1
(-2.2)
P 		117
C 214
(3)
P 		132.6
(-0.2)
P 		85.7
(-2)
P 		64.2
(-2.8)
P 		91.3
(-1.8)
P 		60.3
(-3)
P 		154
avr 163.3
(3) 		146.9
(2) 		67
(-2.8) 		70.9
(-2.5) 		92.9
(-1.2) 		63.4
(-3) 		100
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192066_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15803
Sequence Type Exemplar sequence
Representative Public ID Y58G8A.3 NCBI
Target Description Y58G8A.3 /REP_DB=WormBase Gene ID /WP=CE24480 /TR=Q9N4R5 /GB=AAF60815.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=seven trans-membrane receptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029093(11), NM_001029094(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029093 NCBI Caenorhabditis elegans Y58G8A.4a (Y58G8A.4) mRNA, complete cds. 11/11 None
NM_001029094 NCBI Caenorhabditis elegans Y58G8A.4b (Y58G8A.4) mRNA, complete cds. 11/11 None
SNAP00000024889 ENSEMBL cdna:SNAP chromosome:CEL140:V:277954:281533:-1 11/11 None
GENEFINDER00000024892 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:277063:280217:-1 10/11 None
Y58G8A.4a ENSEMBL cdna:known chromosome:CEL140:V:276819:281545:-1 gene:Y58G8A.4 11/11 None
Y58G8A.4b ENSEMBL cdna:known chromosome:CEL140:V:276840:281533:-1 gene:Y58G8A.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:278823-280800(-) 98.76 100.0
Public Domain and Genome References
Ensembl Y58G8A.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF60815 Hypothetical protein Y58G8A.4a [Caenorhabditis elegans] ref|NP_001024264.1| Y58G8A.4a [Caenorhabditis elegans] 0.0
blast AAT68889 Hypothetical protein Y58G8A.4b [Caenorhabditis elegans] ref|NP_001024265.1| Y58G8A.4b [Caenorhabditis elegans] 0.0
blast CAE58004 Hypothetical protein CBG01072 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 1.9E-49
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 5.1E-44
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 1.2E-30
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001024264.1 7 57-79,92-114,129-151,170-192,226-248,286-308,323-345
NP_001024265.1 7 57-79,92-114,129-151,170-192,226-248,286-308,323-345
Y58G8A.4a 7 57-79,92-114,129-151,170-192,226-248,286-308,323-345
Y58G8A.4b 7 57-79,92-114,129-151,170-192,226-248,286-308,323-345
SNAP00000024889 5 57-79,92-114,129-151,170-192,226-248
GENEFINDER00000024892 4 54-76,110-132,170-192,207-229
Sequence
Target Sequence 
>C. ELEGANS:192066_AT
cgattccagtcacaccatggtacgcgatgaccaaagactgggcattcgggtcagtgatgt
gccatttagttcctttgtcaaattcgtgttcggtgtttgtgacgagttggagcctcactg
caatctccttagataaatttctgcatatcaacgatcccaccaaacaaccagtttctattc
gtcaagcgttggcaataacatttcttatctggatagtctcaacactgataaatctaccgt
atcttatgtctttcgagcacgtcgatggaagcttttacgttcagcccggagaaactccat
actgcgggcacttttgcgacgaggcgaattggcagagcgaaaatagtcgaaagatttacg
gaactacggttatgttgttacagttcgtcgtgccgatggcagtgatcacgtattgctact
tc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGATTCCAGTCACACCATGGTACGC 84 249 200 Antisense
GCATTCGGGTCAGTGATGTGCCATT 689 307 241 Antisense
ATGTGCCATTTAGTTCCTTTGTCAA 600 49 256 Antisense
GAGTTGGAGCCTCACTGCAATCTCC 646 395 303 Antisense
TTTCTGCATATCAACGATCCCACCA 504 669 337 Antisense
CAACCAGTTTCTATTCGTCAAGCGT 374 187 364 Antisense
GTATCTTATGTCTTTCGAGCACGTC 251 455 438 Antisense
TTACGTTCAGCCCGGAGAAACTCCA 159 659 474 Antisense
TCCATACTGCGGGCACTTTTGCGAC 668 589 495 Antisense
TATGTTGTTACAGTTCGTCGTGCCG 496 655 570 Antisense
GGCAGTGATCACGTATTGCTACTTC 590 535 597 Antisense
Sequence Source Affymetrix Proprietary Database