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[192062_at]

Description R08D7.6 /REP_DB=WormBase Gene ID /WP=CE01047 /TR=SW:P30645 /GB=CAA78052.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Cyclic GMP phosphodiesterase
Cluster 6grp_highest_10_10 > 94      6grp_high1_10_10 > 58      4grp_highest_8_8 > 15      4grp_high_8_8 > 14      6grp_highest_nonorm > 86      6grp_total > 49      4grp_total > 09      6grp_highest_sub > Cu      6grp_cgc > 18      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 697
1 2 3 4 range
A 1376
(-3)
P 		1508
(0.4)
P 		1399.7
(-2.4)
P 		1611.6
(3)
P 		236
B 1408
(-3)
P 		1479.1
(-2.4)
P 		1501.4
(-2.2)
P 		2072.6
(3)
P 		665
C 1733
(3)
P 		1588.4
(-0.8)
P 		1677.5
(1.5)
P 		1504.9
(-3)
P 		228
avr 1505.7
(-3) 		1525.2
(-2.5) 		1526.2
(-2.5) 		1729.7
(3) 		224

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 925
1 2 3 4 5 6 range
A 917.7
(0)
P 		1191.5
(2.3)
P 		600.5
(-2.6)
P 		555.9
(-3)
P 		884.2
(-0.3)
P 		1275.5
(3)
P 		720
B 1008
(-0.2)
P 		1006.4
(-0.2)
P 		1053.4
(0.8)
P 		896.6
(-2.4)
P 		867.7
(-3)
P 		1163.1
(3)
P 		295
C 1480.5
(3)
P 		790.7
(-1.9)
P 		716.4
(-2.4)
P 		631.4
(-3)
P 		1161.1
(0.7)
P 		1264
(1.5)
P 		849
avr 1135.4
(1.9) 		996.2
(0.4) 		790.1
(-1.9) 		694.6
(-3) 		971
(0.1) 		1234.2
(3) 		540
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 349
1 2 3 4 range
A 501
(3)
P 		327.4
(-0.9)
P 		365.9
(-0.1)
P 		235.9
(-3)
P 		265
B 449
(3)
P 		365.3
(-2)
P 		347.9
(-3)
P 		441.7
(2.6)
P 		101
C 573.7
(2.8)
P 		456.7
(0.7)
P 		584.7
(3)
P 		253.7
(-3)
P 		331
avr 507.9
(3) 		383.1
(-0.8) 		432.8
(0.7) 		310.4
(-3) 		198

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 422
1 2 3 4 5 6 range
A 169.2
(-2.2)
P 		423.7
(3)
P 		130.8
(-3)
P 		141
(-2.8)
P 		266.8
(-0.2)
P 		310.5
(0.7)
P 		293
B 350.7
(0.5)
P 		335
(-0)
P 		255.8
(-2.4)
P 		237.5
(-3)
P 		352.5
(0.5)
P 		433.7
(3)
P 		196
C 548.8
(3)
P 		241.3
(-1.4)
P 		182.3
(-2.2)
P 		127
(-3)
P 		375.7
(0.5)
P 		423.1
(1.2)
P 		422
avr 356.2
(2.1) 		333.3
(1.5) 		189.6
(-2.4) 		168.5
(-3) 		331.7
(1.4) 		389.1
(3) 		221
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192062_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7850
Sequence Type Exemplar sequence
Representative Public ID R08D7.6 NCBI
Target Description R08D7.6 /REP_DB=WormBase Gene ID /WP=CE01047 /TR=SW:P30645 /GB=CAA78052.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Cyclic GMP phosphodiesterase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027534(11), NM_001027535(11), NM_001027536(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027534 NCBI Caenorhabditis elegans PhosphoDiEsterase family member (pde-2) (pde-2) mRNA, complete cds. 11/11 None
NM_001027535 NCBI Caenorhabditis elegans PhosphoDiEsterase family member (pde-2) (pde-2) mRNA, complete cds. 11/11 None
NM_001027536 NCBI Caenorhabditis elegans PhosphoDiEsterase family member (pde-2) (pde-2) mRNA, complete cds. 11/11 None
SNAP00000003058 ENSEMBL cdna:SNAP chromosome:CEL140:III:8975563:8978955:-1 11/11 None
GENEFINDER00000003061 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8975563:8981261:-1 11/11 None
R08D7.6a ENSEMBL cdna:known chromosome:CEL140:III:8974972:8981654:-1 gene:R08D7.6 11/11 A 
R08D7.6b ENSEMBL cdna:known chromosome:CEL140:III:8975360:8981803:-1 gene:R08D7.6 11/11 None
R08D7.6c ENSEMBL cdna:known chromosome:CEL140:III:8975563:8981803:-1 gene:R08D7.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8975558-8981800(-) 97.39 99.02
Public Domain and Genome References
Ensembl R08D7.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA78052 Hypothetical protein R08D7.6a [Caenorhabditis elegans] ref|NP_001022705.1| PhosphoDiEsterase family member (pde-2) [Caenorhabditis elegans] sp|P30645|PDE2_CAEEL Probable 3',5'-cyclic phosphodiesterase pde-2 0.0
blast CAH19088 Hypothetical protein R08D7.6c [Caenorhabditis elegans] ref|NP_001022707.1| PhosphoDiEsterase family member (pde-2) [Caenorhabditis elegans] 0.0
blast CAE47469 Hypothetical protein R08D7.6b [Caenorhabditis elegans] ref|NP_001022706.1| PhosphoDiEsterase family member (pde-2) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003018 EMBL-EBI GAF 2.1E-16
Pfam IPR002073 EMBL-EBI 35-cyclic nucleotide phosphodiesterase 1.0E-126
Pfam IPR003018 EMBL-EBI GAF 2.1E-16
Pfam IPR002073 EMBL-EBI 35-cyclic nucleotide phosphodiesterase 1.0E-126
Pfam IPR002073 EMBL-EBI 35-cyclic nucleotide phosphodiesterase 1.0E-126
Pfam IPR003018 EMBL-EBI GAF 2.1E-16
Pfam IPR002073 EMBL-EBI 35-cyclic nucleotide phosphodiesterase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:192062_AT
tttgtgctgggttgctctcgatgaagaagttcgaaataatccttctgcaacaaaatacac
tcgagtatttgcgagacgagaatctagaacggagggtacgttttactcagaaaaacaaga
aatggaaatttgcgaaaaacccaaaaaattaatgcttgaatttattttttttaaaaccat
atacgatcgagtgcgaaagcaagacccaagagctgcagaaattgcttcaaaacggttcga
gcctgtatatgcaaacggaagtgttcctcaaacacaagatattttggatcatcgtttcga
tggatatgataagaaatatcaaatcggatgcagtggtggacaaaatcaatcaaacggcaa
acaacaacagattcgttcacttcagaagattaggtcaaagacatctgaagacgatgccct
cctgaaaccaatggacaatggaggatctgttgcagctagttcaactcgtagaggatcccg
tacaccacgacgattatggagacgtgctcgtcaactgatttcatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTGTGCTGGGTTGCTCTCGATGAA 4 665 2169 Antisense
AGTTCGAAATAATCCTTCTGCAACA 159 65 2196 Antisense
GAAAGCAAGACCCAAGAGCTGCAGA 424 359 2363 Antisense
AAAACGGTTCGAGCCTGTATATGCA 583 115 2397 Antisense
GATATTTTGGATCATCGTTTCGATG 694 425 2446 Antisense
ACAACAACAGATTCGTTCACTTCAG 157 111 2529 Antisense
AGACATCTGAAGACGATGCCCTCCT 642 71 2567 Antisense
GGATCTGTTGCAGCTAGTTCAACTC 49 513 2611 Antisense
GTTCAACTCGTAGAGGATCCCGTAC 553 443 2627 Antisense
ATCCCGTACACCACGACGATTATGG 313 37 2643 Antisense
GACGTGCTCGTCAACTGATTTCATC 547 377 2669 Antisense
Sequence Source Affymetrix Proprietary Database