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[192052_at] elongation factor 2

Description ZK328.2 /REP_DB=WormBase Gene ID /WP=CE05066 /GEN=eft-1 /TR=Q23463 /GB=AAA91248.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=elongation factor 2
Cluster 6grp_highest_10_10 > 64      6grp_high1_10_10 > 46      4grp_highest_8_8 > 16      4grp_high_8_8 > 52      6grp_highest_nonorm > 56      6grp_total > 45      4grp_total > 59      6grp_highest_sub > Cu      6grp_cgc > 35      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2264
1 2 3 4 range
A 3050.2
(0.8)
P 		3792.6
(3)
P 		3433.7
(1.9)
P 		1770.6
(-3)
P 		2022
B 2991.4
(1.1)
P 		3606.6
(3)
P 		3603.8
(3)
P 		1639.3
(-3)
P 		1967
C 3116.4
(1.7)
P 		3575.6
(3)
P 		3455.1
(2.6)
P 		1528.4
(-3)
P 		2047
avr 3052.7
(1.2) 		3658.3
(3) 		3497.5
(2.5) 		1646.1
(-3) 		2012

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1163
1 2 3 4 5 6 range
A 2340.1
(3)
P 		2151.7
(1.6)
P 		1963.1
(0.1)
P 		1857.7
(-0.7)
P 		1555.9
(-3)
P 		2285.8
(2.6)
P 		784
B 2658
(3)
P 		2168.2
(0.5)
P 		2403.6
(1.7)
P 		2103.9
(0.1)
P 		1495.2
(-3)
P 		2152.4
(0.4)
P 		1163
C 2056.8
(1)
P 		1938.2
(-0.4)
P 		2221.2
(3)
P 		1983.1
(0.2)
P 		1719.3
(-3)
P 		2221.2
(3)
P 		502
avr 2351.6
(3) 		2086
(0.9) 		2196
(1.8) 		1981.6
(0.1) 		1590.1
(-3) 		2219.8
(2) 		762
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 947
1 2 3 4 range
A 1110.6
(3)
P 		823.3
(1)
P 		897.6
(1.5)
P 		259.2
(-3)
P 		851
B 953.9
(3)
P 		890.7
(2.4)
P 		835.1
(1.8)
P 		349.3
(-3)
P 		605
C 1031.6
(1.9)
P 		1028
(1.9)
P 		1204.2
(3)
P 		257.7
(-3)
P 		947
avr 1032
(3) 		914
(2) 		979
(2.6) 		288.7
(-3) 		743

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 526
1 2 3 4 5 6 range
A 431.4
(-2.9)
P 		765.2
(3)
P 		427.5
(-3)
P 		471.2
(-2.2)
P 		469.5
(-2.3)
P 		556.5
(-0.7)
P 		338
B 924.8
(3)
P 		721.7
(-0.3)
P 		583.7
(-2.6)
P 		557.3
(-3)
P 		607.5
(-2.2)
P 		802.6
(1)
P 		368
C 762.4
(3)
P 		591.4
(0.2)
P 		565.3
(-0.3)
P 		398.8
(-3)
P 		556.3
(-0.4)
P 		743.6
(2.7)
P 		364
avr 706.2
(3) 		692.8
(2.7) 		525.5
(-1.7) 		475.8
(-3) 		544.4
(-1.2) 		700.9
(2.9) 		230
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192052_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7959
Sequence Type Exemplar sequence
Representative Public ID ZK328.2 NCBI
Target Description ZK328.2 /REP_DB=WormBase Gene ID /WP=CE05066 /GEN=eft-1 /TR=Q23463 /GB=AAA91248.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=elongation factor 2
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065907(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065907 NCBI Caenorhabditis elegans Elongation FacTor family member (eft-1) (eft-1) mRNA, complete cds. 11/11 None
SNAP00000038057 ENSEMBL cdna:SNAP chromosome:CEL140:III:6009522:6012667:1 11/11 None
GENEFINDER00000038068 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:6009522:6012667:1 11/11 None
ZK328.2 ENSEMBL cdna:known chromosome:CEL140:III:6009504:6012770:1 gene:ZK328.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6009520-6012666(+) 97.54 100.0
Public Domain and Genome References
Gene Title elongation factor 2
Gene Symbol eft-1
Ensembl ZK328.2
Entrez Gene 175851 Entrez gene
SwissProt Q23463 EMBL-EBI
WormBase CE05066 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:260786_S_AT elongation factor Tu family protein at
CANINE_2:CFA.21139.1.S1_S_AT similar to 116 kDa U5 small nuclear ribonucleoprotein component (U5 snRNP-specific protein, 116 kDa) (U5-116 kDa) (Elongation factor Tu GTP binding domain protein 2) cfa
CANINE_2:CFAAFFX.21738.1.S1_S_AT similar to 116 kDa U5 small nuclear ribonucleoprotein component (U5 snRNP-specific protein, 116 kDa) (U5-116 kDa) (Elongation factor Tu GTP binding domain protein 2) cfa
DROSOPHILA_2:1638331_S_AT dm
DROSOPHILA_2:1624364_AT dm
DROSGENOME1:154447_AT dm
CHICKEN:GGAAFFX.22749.1.S1_AT elongation factor Tu GTP binding domain containing 2 gga
CHICKEN:GGAAFFX.22749.2.S1_S_AT elongation factor Tu GTP binding domain containing 2 gga
CHICKEN:GGAAFFX.21049.1.S1_AT Similar to 116 kDa U5 small nuclear ribonucleoprotein component (U5 snRNP-specific protein, 116 kDa) (U5-116 kDa) gga
HG-U133_PLUS_2:222397_AT elongation factor Tu GTP binding domain containing 2 hs
HG-U133B:222397_AT elongation factor Tu GTP binding domain containing 2 hs
HG-U133_PLUS_2:222398_S_AT elongation factor Tu GTP binding domain containing 2 hs
HG-U133B:222398_S_AT elongation factor Tu GTP binding domain containing 2 hs
HUGENEFL:D21163_AT elongation factor Tu GTP binding domain containing 2 hs
U133_X3P:G12803112_3P_A_AT elongation factor Tu GTP binding domain containing 2 hs
U133_X3P:HS.151787.0.S3_3P_AT elongation factor Tu GTP binding domain containing 2 hs
MOUSE430A_2:1438835_A_AT elongation factor Tu GTP binding domain containing 2 mm
MOE430A:1438835_A_AT elongation factor Tu GTP binding domain containing 2 mm
MOUSE430_2:1438835_A_AT elongation factor Tu GTP binding domain containing 2 mm
MOUSE430A_2:1456107_X_AT elongation factor Tu GTP binding domain containing 2 mm
MOE430A:1456107_X_AT elongation factor Tu GTP binding domain containing 2 mm
MOUSE430_2:1456107_X_AT elongation factor Tu GTP binding domain containing 2 mm
MOE430A:1438836_AT elongation factor Tu GTP binding domain containing 2 mm
MOUSE430A_2:1438836_AT elongation factor Tu GTP binding domain containing 2 mm
MOUSE430_2:1438836_AT elongation factor Tu GTP binding domain containing 2 mm
MU11KSUBB:MSA.15983.0_F_AT elongation factor Tu GTP binding domain containing 2 mm
MU11KSUBB:U97079_S_AT elongation factor Tu GTP binding domain containing 2 mm
MU11KSUBB:MSA.12282.0_S_AT elongation factor Tu GTP binding domain containing 2 mm
MG-U74AV2:101530_AT elongation factor Tu GTP binding domain containing 2 mm
MOE430A:1416557_A_AT elongation factor Tu GTP binding domain containing 2 mm
MOUSE430A_2:1416557_A_AT elongation factor Tu GTP binding domain containing 2 mm
MOUSE430_2:1416557_A_AT elongation factor Tu GTP binding domain containing 2 mm
RG-U34A:RC_AA892940_AT similar to 116 kDa U5 small nuclear ribonucleoprotein component (U5 snRNP-specific protein, 116 kDa) (U5-116 kDa) rn
RG-U34A:RC_AA892940_G_AT similar to 116 kDa U5 small nuclear ribonucleoprotein component (U5 snRNP-specific protein, 116 kDa) (U5-116 kDa) rn
RG-U34C:RC_AA859237_AT similar to 116 kDa U5 small nuclear ribonucleoprotein component (U5 snRNP-specific protein, 116 kDa) (U5-116 kDa) rn
RAE230A:1371698_AT similar to 116 kDa U5 small nuclear ribonucleoprotein component (U5 snRNP-specific protein, 116 kDa) (U5-116 kDa) rn
RAT230_2:1371698_AT similar to 116 kDa U5 small nuclear ribonucleoprotein component (U5 snRNP-specific protein, 116 kDa) (U5-116 kDa) rn
RAE230B:1380224_AT Similar to 116 kDa U5 small nuclear ribonucleoprotein component (U5 snRNP-specific protein, 116 kDa) (U5-116 kDa) rn
RAT230_2:1380224_AT Similar to 116 kDa U5 small nuclear ribonucleoprotein component (U5 snRNP-specific protein, 116 kDa) (U5-116 kDa) rn
RAE230B:1391396_AT Similar to 116 kDa U5 small nuclear ribonucleoprotein component (U5 snRNP-specific protein, 116 kDa) (U5-116 kDa) rn
RAT230_2:1391396_AT Similar to 116 kDa U5 small nuclear ribonucleoprotein component (U5 snRNP-specific protein, 116 kDa) (U5-116 kDa) rn
RG-U34C:RC_AI103321_AT Similar to 116 kDa U5 small nuclear ribonucleoprotein component (U5 snRNP-specific protein, 116 kDa) (U5-116 kDa) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6412 protein biosynthesis inferred from electronic annotation QuickGO AmiGO
6414 translational elongation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3746 translation elongation factor activity inferred from electronic annotation QuickGO AmiGO
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA91248 Elongation factor protein 1 [Caenorhabditis elegans] ref|NP_498308.1| Elongation FacTor family member (eft-1) [Caenorhabditis elegans] 0.0
blast CAE64340 Hypothetical protein CBG09023 [Caenorhabditis briggsae] 0.0
blast AAA21824 putative 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000640 EMBL-EBI Elongation factor G, C-terminal 5.2E-34
Pfam IPR005517 EMBL-EBI Elongation factor G, domain IV 3.5E-21
Pfam IPR004161 EMBL-EBI Elongation factor Tu, domain 2 1.3E-9
Pfam IPR000795 EMBL-EBI Protein synthesis factor, GTP-binding 3.4E-61
Sequence
Target Sequence 
>C. ELEGANS:192052_AT
caatcgccacagaaccactttatcgaggtggaggtcagatgatcccaactgcacgccgat
gtgcctattctgcattccttatggccacgccaagattaatggagccatactatacagtgg
aagttgttgcaccagctgattgtgtagctgctgtatatacagtgttagctaaacgacgtg
gtcacgtcaccactgatgcaccaatgccaggatcacctatgtacactatcagcgcgtaca
ttccagtaatggactcgttcggattcgaaactgatcttcgaattcacacacagggacaag
cattctgtatgtctgctttccatcattggcaactcgtaccaggagatccactcgataaat
ccattgttatcaagactctcgacgtccagccaactccacatctcgccagagagttcatga
tcaagaccagaagacgcaagggtttgtctgaagatgtctccgttaacaagttcttcgatg
atccgatgcttctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAATCGCCACAGAACCACTTTATCG 431 181 2408 Antisense
GGAGGTCAGATGATCCCAACTGCAC 593 515 2437 Antisense
CAACTGCACGCCGATGTGCCTATTC 623 187 2453 Antisense
GTGCCTATTCTGCATTCCTTATGGC 575 477 2468 Antisense
ATGCACCAATGCCAGGATCACCTAT 457 43 2603 Antisense
TCACCTATGTACACTATCAGCGCGT 129 627 2620 Antisense
GCATTCTGTATGTCTGCTTTCCATC 691 307 2707 Antisense
GCTTTCCATCATTGGCAACTCGTAC 103 305 2722 Antisense
AACTCGTACCAGGAGATCCACTCGA 676 137 2738 Antisense
GTTATCAAGACTCTCGACGTCCAGC 263 447 2773 Antisense
GTTCTTCGATGATCCGATGCTTCTC 154 445 2877 Antisense
Sequence Source Affymetrix Proprietary Database