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[192030_at] Initiation factor 2 subunit

Description C01G10.9 /REP_DB=WormBase Gene ID /WP=CE07818 /TR=SW:Q93169 /GB=CAB02711.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Initiation factor 2 subunit
Cluster 6grp_highest_10_10 > 16      6grp_high1_10_10 > 50      4grp_highest_8_8 > 16      4grp_high_8_8 > 52      6grp_highest_nonorm > 62      6grp_total > 51      4grp_total > 59      6grp_highest_sub > 03_04      6grp_high1_sub > 03_04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 610
1 2 3 4 range
A 506.9
(0.7)
P 		657
(2.7)
P 		682.8
(3)
P 		225.3
(-3)
P 		458
B 539.5
(0.6)
P 		737.9
(2.6)
P 		782.3
(3)
P 		172.8
(-3)
P 		610
C 555.1
(0.9)
P 		710.8
(3)
P 		616.7
(1.7)
P 		260.6
(-3)
P 		450
avr 533.8
(0.9) 		701.9
(3) 		693.9
(2.9) 		219.6
(-3) 		482

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 512
1 2 3 4 5 6 range
A 445.9
(-0.2)
P 		461.4
(0)
P 		691.6
(3)
P 		643
(2.4)
P 		344.3
(-1.5)
P 		229.1
(-3)
P 		463
B 394.2
(-0.2)
P 		430.3
(0.3)
P 		547.9
(2.1)
P 		608.6
(3)
P 		356.6
(-0.7)
P 		205
(-3)
P 		404
C 400.7
(0.1)
P 		402.6
(0.2)
P 		580.6
(2.7)
P 		602.9
(3)
P 		374.6
(-0.2)
P 		179.2
(-3)
P 		424
avr 413.6
(0) 		431.4
(0.3) 		606.7
(2.8) 		618.2
(3) 		358.5
(-0.8) 		204.4
(-3) 		414
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 182
1 2 3 4 range
A 184.6
(3)
P 		142.6
(1.3)
P 		178.5
(2.8)
P 		33
(-3)
P 		152
B 172.1
(2.6)
P 		182.2
(3)
P 		181.3
(3)
P 		36.8
(-3)
P 		145
C 183.8
(1.9)
P 		204.3
(2.6)
P 		214.9
(3)
P 		43.9
(-3)
P 		171
avr 180.2
(2.6) 		176.4
(2.4) 		191.6
(3) 		37.9
(-3) 		154

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 108
1 2 3 4 5 6 range
A 82.2
(-1.5)
P 		164.1
(3)
P 		150.6
(2.3)
P 		163.1
(2.9)
P 		103.9
(-0.3)
P 		55.8
(-3)
P 		108
B 137.2
(1.3)
P 		143.2
(1.7)
P 		133.1
(1)
P 		161.2
(3)
P 		144.9
(1.8)
P 		76.4
(-3)
P 		85
C 148.5
(3)
P 		122.8
(1.3)
P 		147.8
(3)
P 		121.2
(1.1)
P 		121.2
(1.1)
P 		60
(-3)
P 		89
avr 122.6
(1.2) 		143.4
(2.6) 		143.8
(2.7) 		148.5
(3) 		123.3
(1.2) 		64.1
(-3) 		84
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192030_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12806
Sequence Type Exemplar sequence
Representative Public ID C01G10.9 NCBI
Target Description C01G10.9 /REP_DB=WormBase Gene ID /WP=CE07818 /TR=SW:Q93169 /GB=CAB02711.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Initiation factor 2 subunit
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074313(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074313 NCBI Caenorhabditis elegans C01G10.9 (C01G10.9) mRNA, complete cds. 11/11 None
GENEFINDER00000004637 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15085373:15086627:1 11/11 None
C01G10.9 ENSEMBL cdna:known chromosome:CEL140:V:15085350:15086652:1 gene:C01G10.9 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000004619 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:15085372-15086627(+) 100.0 100.0
Public Domain and Genome References
Gene Title Initiation factor 2 subunit
Ensembl C01G10.9
Entrez Gene 180016 Entrez gene
SwissProt Q93169 EMBL-EBI
WormBase CE07818 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:18769_AT eukaryotic translation initiation factor 2B family protein / eIF-2B family protein at
ATH1-121501:266033_AT eukaryotic translation initiation factor 2B family protein / eIF-2B family protein at
CANINE_2:CFA.18712.1.S1_S_AT similar to CG11334-PB, isoform B cfa
CANINE_2:CFAAFFX.25423.1.S1_S_AT similar to CG11334-PB, isoform B cfa
CANINE_2:CFAAFFX.25436.1.S1_AT similar to CG11334-PB, isoform B cfa
DROSOPHILA_2:1640926_AT dm
DROSOPHILA_2:1624246_S_AT dm
DROSGENOME1:150944_AT dm
HG-U133_PLUS_2:228077_AT hypothetical protein MGC3207 hs
HG-U133B:228077_AT hypothetical protein MGC3207 hs
HG-U133_PLUS_2:223770_X_AT hypothetical protein MGC3207 hs
HG-U133B:223770_X_AT hypothetical protein MGC3207 hs
HU35KSUBA:RC_AA024518_I_AT hypothetical protein MGC3207 hs
HU35KSUBA:N75238_S_AT hypothetical protein MGC3207 hs
U133_X3P:G12804572_3P_X_AT hypothetical protein MGC3207 hs
U133_X3P:223770_3P_X_AT hypothetical protein MGC3207 hs
U133_X3P:228077_3P_AT hypothetical protein MGC3207 hs
HG-U95B:48518_AT hypothetical protein MGC3207 hs
MOE430A:1417146_AT RIKEN cDNA 2410018C20 gene mm
MOUSE430A_2:1417146_AT RIKEN cDNA 2410018C20 gene mm
MOUSE430_2:1417146_AT RIKEN cDNA 2410018C20 gene mm
MG-U74CV2:165505_AT RIKEN cDNA 2410018C20 gene mm
MG-U74CV2:169759_R_AT RIKEN cDNA 2410018C20 gene mm
MOE430A:1426549_AT RIKEN cDNA 2410018C20 gene mm
MOUSE430A_2:1426549_AT RIKEN cDNA 2410018C20 gene mm
MOUSE430_2:1426549_AT RIKEN cDNA 2410018C20 gene mm
RG-U34C:RC_AA817756_AT similar to hypothetical protein MGC3207 (predicted) rn
RAE230A:1376134_AT similar to hypothetical protein MGC3207 (predicted) rn
RAT230_2:1376134_AT similar to hypothetical protein MGC3207 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6413 translational initiation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5851 eukaryotic translation initiation factor 2B complex inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3743 translation initiation factor activity inferred from electronic annotation QuickGO AmiGO
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB02711 Hypothetical protein C01G10.9 [Caenorhabditis elegans] ref|NP_506714.1| C01G10.9 [Caenorhabditis elegans] sp|Q93169|YQJQ_CAEEL Hypothetical protein C01G10.9 in chromosome V 0.0
blast CAE66321 Hypothetical protein CBG11572 [Caenorhabditis briggsae] 1.0E-177
blast CAD27760 putative translation initiation factor [Anopheles gambiae] 1.0E-74
Protein Domains
Database ID Description E-Value
Pfam IPR000649 EMBL-EBI Initiation factor 2B 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:192030_AT
tatcaagagcttctctcggcattccctgacaaggagaagcttacggtaatgacgatctgc
aacacaggaagcttggctacaatatcctggggtactgctctcggagtcattcgggctctt
cattctgaaaaccgtctgaaattggtttacgttttggagacgagaccctataatcaagga
attaggttgacttccatagagcttcttcatggtgaggtgccgttcaagctgatcactgat
tcaatggcagcatgggcaatgaagaatcaccaggtggattgtgtgctcaccggagccgat
aatgttgcaagaaacggcgatacggctaacaaaatcggaacctatatgcttgctgtcctc
tgcaaacatcacaacatcaacttctatccggttgttccattcacgacgatcaataagaat
attgcaaccggggaggagattaaaattgaagaacgaccatctgcagagatgcttcgagta
aatggagttttaatcggaaattctgaatgtccggtttggaatccagcatttgatgtcacg
ccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATCAAGAGCTTCTCTCGGCATTCC 242 661 493 Antisense
TACAATATCCTGGGGTACTGCTCTC 42 641 570 Antisense
TCATTCGGGCTCTTCATTCTGAAAA 429 619 599 Antisense
AGGTTGACTTCCATAGAGCTTCTTC 522 57 676 Antisense
AGCTTCTTCATGGTGAGGTGCCGTT 151 79 692 Antisense
AGGTGCCGTTCAAGCTGATCACTGA 33 59 707 Antisense
ATTGTGTGCTCACCGGAGCCGATAA 228 3 770 Antisense
CGGAACCTATATGCTTGCTGTCCTC 333 243 828 Antisense
GTCCTCTGCAAACATCACAACATCA 240 477 847 Antisense
TTCTATCCGGTTGTTCCATTCACGA 150 689 874 Antisense
GAATCCAGCATTTGATGTCACGCCG 282 331 1011 Antisense
Sequence Source Affymetrix Proprietary Database