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[192010_s_at] heat shock protein

Description F43E2.8 /REP_DB=WormBase Gene ID /WP=CE07244 /GEN=hsp-4 /TR=O02085 /GB=AAC71123.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=heat shock protein
Cluster 6grp_highest_10_10 > 89      6grp_high1_10_10 > 99      4grp_highest_8_8 > 19      4grp_high_8_8 > 55      6grp_highest_nonorm > 59      6grp_total > 95      4grp_total > 56      6grp_highest_sub > In      6grp_cgc > 78      6grp_cgc_sub > In      6grp_total_sub > Increase      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3439
1 2 3 4 range
A 944.3
(-3)
P 		2712
(0.1)
P 		4338.7
(3)
P 		1351.8
(-2.3)
P 		3394
B 974.3
(-3)
P 		2217.7
(-0.8)
P 		4327.8
(3)
P 		1423
(-2.2)
P 		3354
C 942
(-3)
P 		2101.1
(-1)
P 		4381.3
(3)
P 		1217.2
(-2.5)
P 		3439
avr 953.5
(-3) 		2343.6
(-0.5) 		4349.3
(3) 		1330.7
(-2.3) 		3396

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2387
1 2 3 4 5 6 range
A 2229.1
(-1.8)
P 		2694.1
(-0.4)
P 		1796.8
(-3)
P 		3095.4
(0.7)
P 		3646.2
(2.3)
P 		3880.4
(3)
P 		2084
B 2071.2
(-2.2)
P 		2563.9
(-0.7)
P 		1787
(-3)
P 		2956.7
(0.4)
P 		3605.7
(2.3)
P 		3845.4
(3)
P 		2058
C 2132.1
(-3)
P 		2485.6
(-2)
P 		2166.7
(-2.9)
P 		2852.5
(-0.9)
P 		4174.4
(3)
P 		4143.7
(2.9)
P 		2042
avr 2144.1
(-2.3) 		2581.2
(-1) 		1916.8
(-3) 		2968.2
(0.1) 		3808.8
(2.6) 		3956.5
(3) 		2040
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1329
1 2 3 4 range
A 343.8
(-2.1)
P 		588.7
(-0.5)
P 		1134.2
(3)
P 		197.9
(-3)
P 		936
B 310.7
(-2.9)
P 		547.7
(-0.9)
P 		1002.9
(3)
P 		303.2
(-3)
P 		700
C 311.8
(-2.5)
P 		604.1
(-1.2)
P 		1527
(3)
P 		205.2
(-3)
P 		1322
avr 322.1
(-2.5) 		580.2
(-0.9) 		1221.4
(3) 		235.4
(-3) 		986

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1074
1 2 3 4 5 6 range
A 410.9
(-2.8)
P 		958.2
(1.8)
P 		391.3
(-3)
P 		785.1
(0.3)
P 		1100.3
(3)
P 		944.7
(1.7)
P 		709
B 720.7
(-1.3)
P 		853.4
(-0.6)
P 		433.9
(-3)
P 		783.1
(-1)
P 		1465
(3)
P 		1433.9
(2.8)
P 		1031
C 790.3
(-1.3)
P 		758.4
(-1.5)
P 		551.5
(-3)
P 		573.6
(-2.8)
P 		1350.8
(2.7)
P 		1387.1
(3)
P 		836
avr 640.6
(-1.7) 		856.7
(-0.2) 		458.9
(-3) 		713.9
(-1.2) 		1305.4
(3) 		1255.2
(2.6) 		847
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192010_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4156
Sequence Type Exemplar sequence
Representative Public ID F43E2.8 NCBI
Target Description F43E2.8 /REP_DB=WormBase Gene ID /WP=CE07244 /GEN=hsp-4 /TR=O02085 /GB=AAC71123.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=heat shock protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063135(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063135 NCBI Caenorhabditis elegans Heat Shock Protein family member (hsp-4) (hsp-4) mRNA, complete cds. 11/11 None
SNAP00000044487 ENSEMBL cdna:SNAP chromosome:CEL140:II:7350189:7352403:-1 11/11 None
F43E2.8.1 ENSEMBL cdna:known chromosome:CEL140:II:7350048:7352447:-1 gene:F43E2.8 11/11 None
F43E2.8.2 ENSEMBL cdna:known chromosome:CEL140:II:7350048:7352449:-1 gene:F43E2.8 11/11 None
F43E2.8.3 ENSEMBL cdna:known chromosome:CEL140:II:7350059:7352565:-1 gene:F43E2.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7350185-7352400(-) 100.0 100.0
Public Domain and Genome References
Gene Title heat shock protein
Gene Symbol hsp-4
Ensembl F43E2.8
Entrez Gene 174203 Entrez gene
SwissProt P20163 EMBL-EBI
WormBase CE07244 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:15172_S_AT luminal binding protein 1 (BiP-1) (BP1) at
ATH1-121501:245956_S_AT luminal binding protein 1 (BiP-1) (BP1) at
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6950 response to stress inferred from direct assay QuickGO AmiGO
6950 response to stress inferred from sequence similarity QuickGO AmiGO
6457 protein folding inferred from sequence similarity QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5783 endoplasmic reticulum inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC71123 Heat shock protein protein 4 [Caenorhabditis elegans] ref|NP_495536.1| Heat Shock Protein family member (hsp-4) [Caenorhabditis elegans] sp|P20163|HSP7D_CAEEL Heat shock 70 kDa protein D precursor 0.0
blast CAE67599 Hypothetical protein CBG13144 [Caenorhabditis briggsae] 0.0
blast AAB52671 Heat shock protein protein 3 [Caenorhabditis elegans] ref|NP_509019.1| Heat Shock Protein family member (hsp-3) [Caenorhabditis elegans] sp|P27420|HSP7C_CAEEL Heat shock 70 kDa protein C precursor pir||T15513 heat shock 70K protein C precursor HSP70C - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001023 EMBL-EBI Heat shock protein Hsp70 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:192010_S_AT
tctctaccgctgccgatagtcagagcgctgtatcaatcgttatttatgaaggtgaacgac
caatggttatggacaatcacaagctcggaaacttcgatgtcaccggaatcccaccagctc
cacgcggtgttccacaaattgaggttacattcgaaattgatgtcaacggaatattgcacg
taagcgccgaagacaagggaactggaaacaagaacaagctcacaatcaccaacgaccaca
atcgtctcagtccagaagacatcgagcgcatgatcaatgatgctgacaagtttgctgcag
atgatcaagcccaaaaagagaaggttgagtcacgaaatgagctggaagcatatgcctatc
agatgaaaactcaaatcgccgacaaggaaaaactcggaggaaagctcactgacgaagaca
aggtgtccatcgagtctgccgtcgagagagctattgagtggctcggaagcaaccaagatg
cctctactgaagaaaacaaggagcaaaagaaggaacttgaatctgttgttcaaccaatcg
tctccaaactttactcggcgggag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTCTACCGCTGCCGATAGTCAGAG 84 613 1370 Antisense
GTCAGAGCGCTGTATCAATCGTTAT 208 467 1388 Antisense
GGTTATGGACAATCACAAGCTCGGA 315 507 1434 Antisense
GAAACTTCGATGTCACCGGAATCCC 324 357 1457 Antisense
GGAATATTGCACGTAAGCGCCGAAG 520 533 1537 Antisense
ACCAACGACCACAATCGTCTCAGTC 253 83 1597 Antisense
GTTTGCTGCAGATGATCAAGCCCAA 444 449 1659 Antisense
TCCATCGAGTCTGCCGTCGAGAGAG 476 589 1795 Antisense
GAAGCAACCAAGATGCCTCTACTGA 254 341 1835 Antisense
GTTGTTCAACCAATCGTCTCCAAAC 697 437 1894 Antisense
GTCTCCAAACTTTACTCGGCGGGAG 73 469 1909 Antisense
Sequence Source Affymetrix Proprietary Database