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[192009_at] Yeast DEG-1 protein homolog

Description E02H1.3 /REP_DB=WormBase Gene ID /WP=CE01538 /TR=SW:Q09524 /GB=CAA87378.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=Yeast DEG-1 protein homolog
Cluster 6grp_high1_10_10 > 75      6grp_total > 76      4grp_total > 63      6grp_cgc > 57      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 100
1 2 3 4 range
A 143.4
(3)
P 		109.4
(-0.4)
A 		82.8
(-3)
A 		118.5
(0.5)
P 		61
B 150.2
(-0.1)
P 		120.7
(-3)
A 		180.9
(3)
P 		125.2
(-2.6)
M 		60
C 182.4
(3)
P 		157.7
(-1.3)
A 		178.8
(2.4)
P 		147.9
(-3)
P 		35
avr 158.7
(3) 		129.3
(-3) 		147.5
(0.7) 		130.5
(-2.8) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 179
1 2 3 4 5 6 range
A 146.9
(-1.8)
M 		124.8
(-3)
P 		206.3
(1.5)
P 		154.2
(-1.4)
P 		154.9
(-1.4)
P 		234.3
(3)
P 		110
B 137.6
(-3)
P 		154.9
(-2.2)
P 		229.2
(1)
P 		163.4
(-1.9)
P 		196.4
(-0.4)
P 		275.8
(3)
P 		138
C 128.5
(-3)
P 		192.9
(-0.8)
P 		129.4
(-3)
P 		245
(1)
P 		143.6
(-2.5)
P 		304.1
(3)
P 		176
avr 137.7
(-3) 		157.5
(-2.1) 		188.3
(-0.7) 		187.5
(-0.8) 		165
(-1.8) 		271.4
(3) 		134
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 45
1 2 3 4 range
A 52.2
(3)
P 		23.7
(-1.9)
A 		21.6
(-2.3)
A 		17.3
(-3)
P 		35
B 47.9
(3)
P 		29.8
(-2.1)
A 		41.9
(1.3)
P 		26.7
(-3)
M 		21
C 60.4
(2.7)
P 		45.3
(0.3)
A 		62.3
(3)
P 		24.9
(-3)
P 		37
avr 53.5
(3) 		32.9
(-1.1) 		41.9
(0.7) 		23
(-3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 76
1 2 3 4 5 6 range
A 27.1
(-3)
M 		44.4
(0.5)
P 		44.9
(0.6)
P 		39.1
(-0.6)
P 		46.7
(0.9)
P 		57
(3)
P 		30
B 47.9
(-2.5)
P 		51.6
(-2.2)
P 		55.7
(-1.7)
P 		43.3
(-3)
P 		79.8
(0.7)
P 		102.8
(3)
P 		60
C 47.6
(-1.7)
P 		58.8
(-0.7)
P 		32.9
(-3)
P 		49.3
(-1.6)
P 		46.5
(-1.8)
P 		101.8
(3)
P 		69
avr 40.9
(-3) 		51.6
(-1.6) 		44.5
(-2.5) 		43.9
(-2.6) 		57.7
(-0.8) 		87.2
(3) 		46
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192009_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3620
Sequence Type Exemplar sequence
Representative Public ID E02H1.3 NCBI
Target Description E02H1.3 /REP_DB=WormBase Gene ID /WP=CE01538 /TR=SW:Q09524 /GB=CAA87378.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=Yeast DEG-1 protein homolog
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063661(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063661 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-124) (tag-124) mRNA, complete cds. 11/11 None
SNAP00000041921 ENSEMBL cdna:SNAP chromosome:CEL140:II:9591316:9592764:1 11/11 None
GENEFINDER00000041931 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:9591316:9592764:1 11/11 None
E02H1.3 ENSEMBL cdna:known chromosome:CEL140:II:9591234:9592830:1 gene:E02H1.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9591312-9592761(+) 100.0 100.0
Public Domain and Genome References
Gene Title Yeast DEG-1 protein homolog
Gene Symbol tag-124
Ensembl E02H1.3
Entrez Gene 174509 Entrez gene
SwissProt Q09524 EMBL-EBI
WormBase CE01538 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:255985_AT tRNA pseudouridine synthase family protein at
CANINE:1589542_AT similar to tRNA pseudouridine synthase 3 (tRNA-uridine isomerase 3) (tRNA pseudouridylate synthase 3) cfa
CANINE_2:CFAAFFX.16461.1.S1_AT similar to tRNA pseudouridine synthase 3 (tRNA-uridine isomerase 3) (tRNA pseudouridylate synthase 3) cfa
CANINE_2:CFAAFFX.16461.1.S1_S_AT similar to tRNA pseudouridine synthase 3 (tRNA-uridine isomerase 3) (tRNA pseudouridylate synthase 3) cfa
DROSGENOME1:152572_AT dm
DROSOPHILA_2:1635805_AT dm
CHICKEN:GGAAFFX.22673.1.S1_AT similar to hypothetical protein FKSG32 gga
HG-U133_PLUS_2:221277_S_AT pseudouridylate synthase 3 hs
HG-U133A:221277_S_AT pseudouridylate synthase 3 hs
HG-U133A_2:221277_S_AT pseudouridylate synthase 3 hs
HU35KSUBD:RC_AA432136_AT pseudouridylate synthase 3 hs
U133_X3P:G13775233_3P_S_AT pseudouridylate synthase 3 hs
HG-U95B:56045_AT pseudouridylate synthase 3 hs
MOUSE430_2:1418491_A_AT pseudouridine synthase 3 mm
MOE430A:1418491_A_AT pseudouridine synthase 3 mm
MOUSE430A_2:1418491_A_AT pseudouridine synthase 3 mm
RAE230B:1395850_AT similar to CG3045-PA (predicted) rn
RAT230_2:1395850_AT similar to CG3045-PA (predicted) rn
RG-U34A:RC_AA894165_AT Similar to CG3045-PA (predicted) rn
YEAST_2:1779033_AT Non-essential tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8033 tRNA processing inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4730 pseudouridylate synthase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA87378 Hypothetical protein E02H1.3 [Caenorhabditis elegans] ref|NP_496062.3| Temporarily Assigned Gene name family member (tag-124) [Caenorhabditis elegans] sp|Q09524|YQN3_CAEEL Probable tRNA pseudouridine synthase E02H1.3 (tRNA-uridine isomerase) (tRNA pseudouridylate synthase) 0.0
blast CAE57910 Hypothetical protein CBG00960 [Caenorhabditis briggsae] 1.0E-173
blast CAG00940 unnamed protein product [Tetraodon nigroviridis] 5.0E-64
Protein Domains
Database ID Description E-Value
Pfam IPR001406 EMBL-EBI tRNA pseudouridine synthase 5.2E-14
Pfam IPR001406 EMBL-EBI tRNA pseudouridine synthase 2.8E-28
Sequence
Target Sequence 
>C. ELEGANS:192009_AT
gaacaagtatcgactcatccagaaaatgatatgtattcaatggtggagctcactgtatct
ggaagtggtttcctttggcatatgattcgatacatagtcacgatccttcaagaaattgga
agagaaaatgaacaaccatcattaatctcacagcttcttgacctgaaaaaatatccgagc
cggccgcagtacaccttagcctcggacactcctctatgcttattcgattgtggatataaa
tctgaagatgttgaatggaaagttcatgactatacactgaaaagtacagtgaccggacta
caaaaaacctgggcgacgtatcaggcgagaagtcgaatgatggaaaatatgctcggagaa
ctgactggaatggctgaattcagttcaggagatgccaataaaggacttcacgaatttgta
caagatcgtccgataccttcaaattac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACAAGTATCGACTCATCCAGAAA 324 353 673 Antisense
ATGGTGGAGCTCACTGTATCTGGAA 437 51 712 Antisense
GGAAGTGGTTTCCTTTGGCATATGA 677 531 733 Antisense
ATACATAGTCACGATCCTTCAAGAA 109 25 762 Antisense
GAACAACCATCATTAATCTCACAGC 256 353 802 Antisense
TAATCTCACAGCTTCTTGACCTGAA 67 637 815 Antisense
AAAAAATATCCGAGCCGGCCGCAGT 498 127 838 Antisense
GAAAAGTACAGTGACCGGACTACAA 584 351 951 Antisense
AAACCTGGGCGACGTATCAGGCGAG 308 123 977 Antisense
GCTCGGAGAACTGACTGGAATGGCT 510 289 1023 Antisense
AGATCGTCCGATACCTTCAAATTAC 423 67 1095 Antisense
Sequence Source Affymetrix Proprietary Database