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[192005_at]

Description E01H11.1 /REP_DB=WormBase Gene ID /WP=CE02586 /GEN=kin-11 /TR=Q19025 /GB=AAA68710.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=Protein kinase C
Cluster 6grp_highest_10_10 > 10      6grp_high1_10_10 > 07      4grp_highest_8_8 > 49      4grp_high_8_8 > 49      6grp_highest_nonorm > 60      6grp_total > 04      4grp_total > 54      6grp_cgc > 10      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 443
1 2 3 4 range
A 397.4
(2.9)
P 		254.1
(-1.6)
P 		401.4
(3)
P 		210.5
(-3)
P 		191
B 555.6
(3)
P 		351.6
(-0)
P 		242.2
(-1.7)
P 		152.6
(-3)
P 		403
C 595.9
(3)
P 		365.8
(-0.5)
P 		318
(-1.2)
P 		196.2
(-3)
P 		400
avr 516.3
(3) 		323.8
(-0.5) 		320.5
(-0.6) 		186.4
(-3) 		330

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 425
1 2 3 4 5 6 range
A 626.2
(3)
P 		417.9
(-0.4)
P 		472.5
(0.5)
P 		275.9
(-2.7)
P 		508
(1.1)
P 		256.4
(-3)
P 		370
B 572.2
(3)
P 		415.4
(0.5)
P 		201.6
(-3)
P 		516.3
(2.1)
P 		225.2
(-2.6)
P 		283
(-1.7)
P 		371
C 426.2
(3)
P 		404.8
(2.3)
P 		380.5
(1.5)
P 		350.4
(0.6)
P 		353.7
(0.7)
P 		240.3
(-3)
P 		186
avr 541.5
(3) 		412.7
(0.3) 		351.5
(-1) 		380.9
(-0.4) 		362.3
(-0.8) 		259.9
(-3) 		282
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 167
1 2 3 4 range
A 144.7
(3)
P 		55.2
(-1.7)
P 		104.9
(0.9)
P 		30.8
(-3)
P 		114
B 177.2
(3)
P 		86.8
(-0.7)
P 		56.1
(-2)
P 		32.5
(-3)
P 		145
C 197.3
(3)
P 		105.2
(-0.4)
P 		110.8
(-0.2)
P 		33.1
(-3)
P 		164
avr 173.1
(3) 		82.4
(-0.9) 		90.6
(-0.5) 		32.1
(-3) 		141

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 150
1 2 3 4 5 6 range
A 115.4
(0.5)
P 		148.6
(2.7)
P 		102.9
(-0.3)
P 		70
(-2.5)
P 		153.3
(3)
P 		62.4
(-3)
P 		91
B 199.1
(3)
P 		138.3
(0.6)
P 		49
(-3)
P 		136.7
(0.5)
P 		91.5
(-1.3)
P 		105.5
(-0.7)
P 		150
C 158
(3)
P 		123.5
(0.6)
P 		96.8
(-1.2)
P 		70.5
(-3)
P 		114.5
(0)
P 		80.4
(-2.3)
P 		88
avr 157.5
(3) 		136.8
(1.3) 		82.9
(-3) 		92.4
(-2.2) 		119.8
(-0) 		82.8
(-3) 		75
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192005_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16119
Sequence Type Exemplar sequence
Representative Public ID E01H11.1 NCBI
Target Description E01H11.1 /REP_DB=WormBase Gene ID /WP=CE02586 /GEN=kin-11 /TR=Q19025 /GB=AAA68710.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=Protein kinase C
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029347(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029347 NCBI Caenorhabditis elegans Protein Kinase C family member (pkc-2) (pkc-2) mRNA, complete cds. 11/11 None
E01H11.1c ENSEMBL cdna:known chromosome:CEL140:X:9376201:9385656:1 gene:E01H11.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
181166 3 1 NM_001029345 175780_at (11), 175781_s_at (11)
NM_001029346 175779_at (11), 192006_s_at (11)
NM_001029347 175781_s_at (11), 186204_at (11), 192005_at (11), 192006_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:9378798-9385656(+) 100.0 100.0
Public Domain and Genome References
Ensembl E01H11.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA68709 Protein kinase c protein 2, isoform c [Caenorhabditis elegans] ref|NP_001024518.1| Protein Kinase C family member (pkc-2) [Caenorhabditis elegans] 0.0
blast T15903 protein kinase C homolog - Caenorhabditis elegans 0.0
blast CAE69829 Hypothetical protein CBG16150 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 2.0E-68
hanks 1.2.3 AGC group; AGC I Diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family; DPKC53b 1.0E-126
Protein Domains
Database ID Description E-Value
Pfam IPR000008 EMBL-EBI C2 domain 1.6E-37
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 2.0E-24
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 6.2E-24
Pfam IPR000719 EMBL-EBI Protein kinase 3.0E-81
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 2.7E-21
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 7.5E-21
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001024518.1 3 588-610,824-843,848-870
E01H11.1c 3 588-610,824-843,848-870
Sequence
Target Sequence 
>C. ELEGANS:192005_AT
gatgtagaaccccatattttttatgtactatttattttcttaattttgcgtcctttttta
ttgcttccatatctttcacatcttcaacttacactctccgccattcattccccacttcta
ttactatcagcttctgttttttttaaatttctttttaaaaacaatttttctcagtctccc
tataataaaatcaaagttttgcttaaatccgccgatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGTAGAACCCCATATTTTTTATG 601 407 2242 Antisense
TTATGTACTATTTATTTTCTTAATT 352 681 2262 Antisense
TTTGCGTCCTTTTTTATTGCTTCCA 318 669 2286 Antisense
TTATTGCTTCCATATCTTTCACATC 266 679 2299 Antisense
TTCCATATCTTTCACATCTTCAACT 232 701 2306 Antisense
TCTTTCACATCTTCAACTTACACTC 694 615 2313 Antisense
TCCCCACTTCTATTACTATCAGCTT 184 597 2350 Antisense
ACTTCTATTACTATCAGCTTCTGTT 647 103 2355 Antisense
TATCAGCTTCTGTTTTTTTTAAATT 665 659 2366 Antisense
ATTTTTCTCAGTCTCCCTATAATAA 456 15 2405 Antisense
AAAGTTTTGCTTAAATCCGCCGATG 293 121 2434 Antisense
Sequence Source Affymetrix Proprietary Database